The Surgical Treatment for Corneal Epithelial Stem Cell Deficiency, Corneal Epithelial Defect, and Peripheral Corneal Ulcer

2010 ◽  
pp. 239-246 ◽  
Author(s):  
N. Koizumi ◽  
S. Kinoshita
Keyword(s):  
Stem Cell ◽  
Surgical Treatment ◽  
Corneal Ulcer ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell ◽  
Corneal Epithelial Defect

scholarly journals Subconjunctival Injection of Transdifferentiated Oral Mucosal Epithelial Cells for Limbal Stem Cell Deficiency in Rats

2020 ◽  
pp. 002215542098007
Author(s):  
Yu-Ting Xiao ◽  
Hua-Tao Xie ◽  
Xin Liu ◽  
Chao-Ye Duan ◽  
Jing-Yu Qu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Pigment Epithelium ◽  
Limbal Stem Cell Deficiency ◽  
Subconjunctival Injection ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Limbal Stem Cell

Rat limbal niche cells (LNCs) have been proven to induce transdifferentiation of oral mucosal epithelial cells (OMECs) into corneal epithelial-like cells termed transdifferentiated oral mucosal epithelial cells (T-OMECs). This investigation aimed to evaluate the effect of subconjunctival T-OMEC injections on alkali-induced limbal stem cell deficiency (LSCD) in rats. LNCs were cocultured with OMECs in the Transwell system to obtain T-OMECs, with NIH-3T3 cells serving as a control. Subconjunctival injection of single T-OMEC or OMEC suspension was performed immediately after corneal alkali injury. T-OMECs were prelabeled with the fluorescent dye CM-DiI in vitro and tracked in vivo. Corneal epithelial defect, opacity, and neovascularization were quantitatively analyzed. The degree of corneal epithelial defect (from day 1 onward), opacity (from day 5 onward), and neovascularization (from day 2 onward) was significantly less in the T-OMEC group than in the OMEC group. Cytokeratin 12 (CK12), pigment epithelium–derived factor, and soluble fms-like tyrosine kinase-1 were expressed at a higher rate following T-OMEC injection. Some CM-DiI-labeled cells were found to be coexpressed with CK12, Pax6, and ΔNp63α in the corneal epithelium after subconjunctival injection. Subconjunctival injection of T-OMECs prevents conjunctival invasion and maintains a normal corneal phenotype, which might be a novel strategy in the treatment of LSCD:

Efficacy of honey based ophthalmic formulation in patients with corneal ulcer; A randomized clinical trial

2020 ◽  
Vol 17 ◽  
Author(s):  
Mahmood Nejabat ◽  
Kazem Soltanzadeh ◽  
Masoud Yasemi ◽  
Saeid Daneshamouz ◽  
Amin Reza Akbarizadeh ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Foreign Body ◽  
Randomized Clinical Trial ◽  
Standard Treatment ◽  
Corneal Ulcer ◽  
Complete Healing ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Ophthalmic Formulation

Aim: The aim of this study was to evaluate the efficacy of honey-based ophthalmic drop in patients with foreign body induced corneal ulcer. Background: Honey is traditionally used for the skin, mucosal and corneal ulcers. Its use is well studied in human skin and mucosal ulcers and animal model of corneal ulcer with promising effects. Methods: In this randomized clinical trial, 50 patients with foreign body induced corneal ulcer were allocated to receive 70% sterile honey-based ophthalmic formulation or 0.3% ophthalmic ciprofloxacin, as the standard treatment every 6 hours. All patients were examined for the size of corneal epithelial defect, corneal infiltration and depth and followed on a daily basis until complete healing. Duration for complete healing was considered as the outcome measure. Smear, culture, antibiogram and minimum inhibition concentration (MIC) test were performed for honey and ciprofloxacin in all patients. Results: The average durations of complete healing of corneal epithelial defect in the honey and ciprofloxacin groups were 3.88 ± 3.44 vs. 6.32 ± 3.69days, respectively (p=0.020). No significant difference was observed between the two groups regarding the average duration of healing of corneal infiltration (8.12 ±1.94 days vs. 8.64±2.15 days, p=0.375). MIC of honey for pseudomonas aeruginosa was 60%w/w, for E.Coli 40% w/w, and for staphylococcus aureus 30% w/w. Conclusion: Honey based ophthalmic drop can acceleratethe corneal epithelial defect healing in patients with foreign body induced corneal ulcer, compared to ophthalmic ciprofloxacin as the standard treatment.

The Corneal Epithelial Stem Cell Marker - Novel Combination of High Expression of p63 and a Large N/C Ratio

2008 ◽  
Vol 1 (3) ◽  
pp. 95-105
Author(s):  
Arpitha P. ◽  
Prajna N. V. ◽  
Srinivasan M. ◽  
Muthukkaruppan V. R.
Keyword(s):  
Stem Cell ◽  
High Expression ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell ◽  
Large N

scholarly journals Inhibition of mTOR by Rapamycin Aggravates Corneal Epithelial Stem Cell Deficiency by Upregulating Inflammatory Response

Stem Cells ◽  
2019 ◽  
Vol 37 (9) ◽  
pp. 1212-1222 ◽  
Author(s):  
Jong Woo Park ◽  
Jung Hwa Ko ◽  
Bo Hee Kim ◽  
Jin Suk Ryu ◽  
Hyun Ji Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Inflammatory Response ◽  
Corneal Epithelial ◽  
Epithelial Stem Cell

Severe Corneal Complications Associated With Corneal Lubricant Used During Surgery

2017 ◽  
Vol 1 (3) ◽  
pp. 187-190
Author(s):  
Andrew C. Lee ◽  
E. Mitchel Opremcak ◽  
Christiane Hunt ◽  
Juan A. Astruc ◽  
Brian Toussaint ◽  
...  
Keyword(s):  
Treatment Method ◽  
The United States ◽  
Posterior Segment ◽  
Epithelial Defect ◽  
Chemical Burns ◽  
Corneal Epithelial ◽  
Corneal Injury ◽  
Corneal Epithelial Defect ◽  
Time Period ◽  
Litmus Paper

Purpose: To present a series of cases of corneal chemical burns associated with the use of likely improperly buffered corneal lubricant gel, Goniovisc, during uncomplicated, posterior segment surgery. Methods: Cases of postoperative corneal complications following surgeries in which Goniovisc was used were collected from 6 retina practices across the United States between January and August 2016. The preoperative diagnosis, type of procedure, postoperative day 1 corneal findings, treatment method, and time to resolution of the corneal irregularity were recorded. pH testing of Goniovisc lots from this time period was performed using Hydrion litmus paper. Results: A total of 157 eyes of 156 patients were found to have corneal complications following relatively simple posterior segment surgeries in which Goniovisc was used. Findings included one or more of the following: corneal epithelial defect (84% of cases), Descemet folds (34%), stromal edema (14%), and superficial punctate keratitis (3%). Time to resolution ranged from 2 to 90 days (mean, 12 days; median, 7 days). Treatment methods included artificial tears (37%), bandage contact lens (28%), antibiotic drops (17%), antibiotic ointment (16%), and patching (9%). Two cases were treated with amniotic membrane grafts. One case required a penetrating keratoplasty due to an old laser-assisted in situ keratomileusis (LASIK) flap with a nonhealing epithelial defect that scarred significantly. Litmus testing of Goniovisc lots from this time period revealed a pH of 4 to 5. Conclusion: Chemical burns secondary to improperly buffered corneal lubricant solution should be considered in the differential when corneal injury is encountered following posterior segment surgery.

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