Protein Kinase CK2 is a Constitutively Active Enzyme that Promotes Cell Survival: Strategies to Identify CK2 Substrates and Manipulate its Activity in Mammalian Cells

2010 ◽  
pp. 471-493 ◽  
Author(s):  
Jacob P. Turowec ◽  
James S. Duncan ◽  
Ashley C. French ◽  
Laszlo Gyenis ◽  
Nicole A. St. Denis ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Survival ◽  
Mammalian Cells ◽  
Protein Kinase Ck2 ◽  
Active Enzyme ◽  
Survival Strategies ◽  
Kinase Ck2 ◽  
Constitutively Active

scholarly journals Targeting protein kinase CK2 suppresses bladder cancer cell survival via the glucose metabolic pathway

Oncotarget ◽  
2016 ◽  
Vol 7 (52) ◽  
pp. 87361-87372 ◽  
Author(s):  
Xiaolei Zhang ◽  
Xiao Yang ◽  
Chengdi Yang ◽  
Peng Li ◽  
Wenbo Yuan ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Protein Kinase ◽  
Cell Survival ◽  
Cancer Cell ◽  
Metabolic Pathway ◽  
Protein Kinase Ck2 ◽  
Bladder Cancer Cell ◽  
Cancer Cell Survival ◽  
Kinase Ck2

scholarly journals Phosphorylation of Serine 239 of Groucho/TLE1 by Protein Kinase CK2 Is Important for Inhibition of Neuronal Differentiation

2004 ◽  
Vol 24 (19) ◽  
pp. 8395-8407 ◽  
Author(s):  
Hugh N. Nuthall ◽  
Kerline Joachim ◽  
Stefano Stifani
Keyword(s):  
Protein Kinase ◽  
Neuronal Differentiation ◽  
Mammalian Cells ◽  
Protein Kinase Ck2 ◽  
Molecular Mechanisms ◽  
Transcription Repression ◽  
Transcriptional Corepressors ◽  
Kinase Ck2 ◽  
Nuclear Association

ABSTRACT Transcriptional corepressors of the Groucho (Gro)/TLE family play important roles during a variety of developmental pathways, including neuronal differentiation. In particular, they act as negative regulators of neurogenesis, together with Hairy/Enhancer of split (Hes) DNA-binding proteins. The interaction with Hes1 leads to Gro/TLE hyperphosphorylation and increased transcription repression activity in mammalian cells, but the underlying molecular mechanisms are poorly characterized. We now show that Gro/TLE1 is phosphorylated in vivo by protein kinase CK2. This phosphorylation occurs at serine 239 within the conserved CcN domain present in all Gro/TLE family members. Mutation of serine 239 into alanine decreases Hes1-induced hyperphosphorylation of Gro/TLE1 and also reduces its nuclear association and transcription repression activity. We demonstrate further that Gro/TLE1 inhibits the transition of cortical neural progenitors into neurons and that its antineurogenic activity is inhibited by a serine-239-alanine mutation but not by a serine-239-glutamate mutation. These results suggest that CK2 phosphorylation of serine 239 of Gro/TLE1 is important for its function during neuronal differentiation.

scholarly journals Inducible Expression of Protein Kinase CK2 in Mammalian Cells

1999 ◽  
Vol 274 (20) ◽  
pp. 14406-14414 ◽  
Author(s):  
Greg Vilk ◽  
Ronald B. Saulnier ◽  
Rebecca St. Pierre ◽  
David W. Litchfield
Keyword(s):  
Protein Kinase ◽  
Mammalian Cells ◽  
Protein Kinase Ck2 ◽  
Inducible Expression ◽  
Kinase Ck2

Protein kinase CK2 catalyzes tyrosine phosphorylation in mammalian cells

2008 ◽  
Vol 20 (11) ◽  
pp. 1942-1951 ◽  
Author(s):  
Greg Vilk ◽  
Jane E. Weber ◽  
Jacob P. Turowec ◽  
James S. Duncan ◽  
Chenggang Wu ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Tyrosine Phosphorylation ◽  
Mammalian Cells ◽  
Protein Kinase Ck2 ◽  
Kinase Ck2
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