Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix

1987 ◽  
pp. 1-27 ◽  
Author(s):  
RANDALL H. KRAMER ◽  
MARVIN A. KARASEK ◽  
KLAUS G. BENSCH
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Isolation And Characterization ◽  
Subendothelial Matrix

Isolation and characterization of vascular endothelial cells derived from fetal tooth buds of miniature swine

2013 ◽  
Vol 49 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Masanori Nasu ◽  
Taka Nakahara ◽  
Noriko Tominaga ◽  
Yuichi Tamaki ◽  
Yoshiaki Ide ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Miniature Swine ◽  
Isolation And Characterization ◽  
Tooth Buds

Molecular cloning and characterization of antigens expressed on rat tumor vascular endothelial cells

2000 ◽  
Vol 86 (6) ◽  
pp. 799-805 ◽  
Author(s):  
Kenji Taniguchi ◽  
Naoki Harada ◽  
Iwao Ohizumi ◽  
Michiko Kinoshita ◽  
Yasuo Tsutsumi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Molecular Cloning ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Rat Tumor

Human Vascular Endothelial Cells Catabolise Exogenous Glycosaminoglycans by a Novel Route

1992 ◽  
Vol 67 (04) ◽  
pp. 468-472 ◽  
Author(s):  
Joan Dawes ◽  
Duncan S Pepper
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Carbohydrate Chain ◽  
Heparan Sulphate ◽  
Vascular Endothelial ◽  
Dermatan Sulphate ◽  
Cellular Fraction ◽  
Subendothelial Matrix ◽  
Pentosan Polysulphate ◽  
Carbohydrate Chains

SummaryHeparin and other anticoagulant glycosaminoglycans were radiolabelled with 125I and their catabolism by human vascular endothelial cells in culture was studied. Heparin, heparan sulphate and pentosan polysulphate were associated with the cellular fraction and incorporated into the subendothelial matrix, but dermatan sulphate was not found in either fraction. High molecular weight, fully desulphated carbohydrate chains were major catabolic products of all those glycosaminoglycans which were taken up by the cells. Pentosan polysulphate was not degraded further, but the catabolism of heparan sulphate, and to a lesser extent that of heparin, also yielded small oligosaccharides. Thus the first step in catabolism of exogenous glycosaminoglycans by human vascular endothelial cells appears to be complete desul-phation, which destroys their biological activity, followed by depolymerisation of the carbohydrate chain. This alternative to the sequential action of lysosomal exoenzymes is dependent upon binding to the cell; thus dermatan sulphate, which is not associated with the cellular fraction, is not catabolised.

scholarly journals Isolation and Characterization of Antibodies Induced by Immunization with TNF-α Inducible Globotetraosylceramide

2020 ◽  
Vol 21 (10) ◽  
pp. 3632
Author(s):  
Tetsuya Okuda
Keyword(s):  
Inflammatory Responses ◽  
Vascular Endothelial Cells ◽  
Hybridoma Cells ◽  
Vascular Endothelial ◽  
Long Chain Fatty Acids ◽  
Isolation And Characterization ◽  
Tnf Α ◽  
Antibody Induction ◽  
Biochemical Analyses

Glycosphingolipids containing very-long-chain fatty acids (VLCFAs) regulate several immune responses, such as cytokine production, immune signaling, and antibody induction. We previously reported that stimulation with an inflammatory mediator, TNF-α, promotes the expression of glycosphingolipids in vascular endothelial cells. The major component is globotetraosylceramide containing VLCFAs (Gb4Cer-VLCFAs), but its role in inflammatory responses has not been fully investigated. In this study, the antibody-inducing properties of Gb4Cer-VLCFAs were analyzed using serum and hybridoma cells generated from Gb4Cer-VLCFA-immunized mice. The reactivity of serum antibodies against Gb4Cer indicated that immunization with Gb4Cer-VLCFAs immediately induced the production of anti-Gb4Cer antibodies. Over 81% of hybridomas generated from the splenocytes of an immunized mouse produced anti-Gb4Cer antibodies, a subset of which recognized an epitope shared by Gb4Cer and its precursor globotriaosylceramide (Gb3Cer). Further biochemical analyses of established monoclonal antibodies revealed that these antibodies included IgM and IgG3, which specifically react with Gb4Cer and Gb3Cer. These results indicate that immunization with Gb4Cer-VLCFAs can efficiently induce the production of anti-Gb4Cer and -Gb3Cer antibodies by B cells.

Characterization of endothelin secretion by vascular endothelial cells

1990 ◽  
Vol 167 (1) ◽  
pp. 294-300 ◽  
Author(s):  
Terry D. Hexum ◽  
Carl Hoeger ◽  
Jean E. Rivier ◽  
Andrew Baird ◽  
Marvin R. Brown
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial
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