Embryo rescue and chromosomal manipulations

New evidence is described on one way crossing barriers between Aigeiros and Tacamahaca species, gained in experimental full-sib crosses. The technique of crosses and embryo rescue are described. In some crosses of questionable result, the embryo rescue was successful. The possible genetic mechanisms underlying the crossing barriers are discussed. Key words: Populus, hydridization, embryo rescue, crossing barriers

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In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.14577 ◽

2015 ◽

Vol 14 (27) ◽

pp. 2191-2201

Author(s):

Buttibwa Mary ◽

S Kawuki Robert ◽

K Tugume Arthur ◽

Akol Jacinta ◽

Magambo Stephen ◽

...

Keyword(s):

Plant Regeneration ◽

Manihot Esculenta ◽

Embryo Rescue ◽

Manihot Esculenta Crantz ◽

Irradiated Pollen ◽

Self Pollination

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Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.13180 ◽

2014 ◽

Vol 13 (14) ◽

pp. 1524-1528 ◽

Cited By ~ 6

Author(s):

Yan Huabing ◽

Lu Liuying ◽

Alzate Adriana ◽

Ceballos Hernan ◽

Hershey Clair ◽

...

Keyword(s):

Embryo Development ◽

Manihot Esculenta ◽

Embryo Rescue ◽

Manihot Esculenta Crantz

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Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202010000300008 ◽

2010 ◽

Vol 22 (3) ◽

pp. 209-215 ◽

Cited By ~ 12

Author(s):

Zanderluce G. Luis ◽

Kadja Milena G. Bezerra ◽

Jonny Everson Scherwinski-Pereira

Keyword(s):

Oil Palm ◽

Embryo Rescue ◽

Morphological Characteristics ◽

Growth Conditions ◽

Epidermal Cells ◽

In Vitro Cultivation ◽

Vascular Bundles ◽

Anatomical Plasticity ◽

Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

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Interspecific Hybridization Of Red Clover (Trifolium Pratense L.) With Alsike Clover (Trifolium Hybndum L.) Using In Vitro Embryo Rescue

Genetic Manipulation in Plant Breeding ◽

10.1515/9783110871944-081 ◽

1986 ◽

pp. 469-472

Keyword(s):

Interspecific Hybridization ◽

Trifolium Pratense ◽

Embryo Rescue ◽

Red Clover ◽

Trifolium Pratense L

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Embryo Rescue

Plant Development and Biotechnology ◽

10.1201/9780203506561.ch18 ◽

2004 ◽

pp. 235-239 ◽

Cited By ~ 6

Author(s):

Sandra Reed

Keyword(s):

Embryo Rescue

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Development and identification of new syntheticT. turgidum–T. monococcumamphiploids

Plant Genetic Resources ◽

10.1017/s1479262118000175 ◽

2018 ◽

Vol 16 (6) ◽

pp. 555-563 ◽

Cited By ~ 1

Author(s):

Hongyu Li ◽

Xiaojuan Liu ◽

Minghu Zhang ◽

Zhen Feng ◽

Dengcai Liu ◽

...

Keyword(s):

Bread Wheat ◽

Stripe Rust ◽

Triticum Turgidum ◽

Embryo Rescue ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Colchicine Treatment ◽

Hormone Treatment ◽

Stripe Rust Resistance ◽

Adult Plant

AbstractTriticum monococcumssp.monococcumhas useful traits for bread wheat improvement. The synthesis ofTriticum turgidum–T. monococcumamphiploids is an essential step for transferring genes fromT. monococcuminto bread wheat. In this study, 264 wide hybridization combinations were done by crossing 60T. turgidumlines belonging to five subspecies with 83T. monococcumaccessions. Without embryo rescue and hormone treatment, from the 10,810 florets pollinated, 1983 seeds were obtained, with a mean crossability of 18.34% (range 0–89.29%). Many hybrid seeds (90.73%, 923/1017) could germinate and produce plants. A total of 56 new amphiploids (AABBAmAm) were produced by colchicine treatment ofT. turgidum×T. monococcumF1hybrids. The chromosome constitution of amphiploids was characterized by fluorescencein situhybridization using oligonucleotides probes with different chromosome and sub-chromosome specificities. Sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis indicated that theGlu-A1m-b,Glu-A1m-c,Glu-A1m-dandGlu-A1m-hproteins ofT. monococcumwere expressed in some amphiploids. Despite resistance reduction in several cases, 45 out of 56 amphiploids exhibited resistance to the current predominant Chinese stripe rust races at both the seedling and adult plant stage. These novel amphiploids provide new germplasm for the potential improvement of bread wheat quality and stripe rust resistance.

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