Bioremediation of contaminated soil with plant growth rhizobium bacteria

Effect of EDTA and EDDS on phytoremediation of Pb- and Zn- contaminated soil by Brassica Juncea was investigated in this work. Especially, the effect of the kind and the method of adding chelating agent was investigated during the plant growth. Plants were grown in an environmental control system. The biomass of the whole plant was weighed, and the uptake of Pb and Zn in shoot and root were determined using ICP-AES. Consequently, the following matters have been obtained: (1) Both EDTA and EDDS significantly enhanced the translocation of metals (Pb and Zn) in soil from root to shoot. Furthermore, the two chelating agents resulted in a sharply biomass loss for more than 30% of the control. As a result, the total uptake amount of metals by Brassica Juncea was decreased (except the uptake of Pb with the addition of 3.0 mmol•kg-1 EDTA). (2) EDDS showed the higher inhibition for the growth of Brassica Juncea than EDTA. (3) The method for adding EDTA and EDDS at several times separately did not necessarily increase the uptake of heavy metals.

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Root-induced changes of Zn and Pb dynamics in the rhizosphere of sunflower with different plant growth promoting treatments in a heavily contaminated soil

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2017.08.045 ◽

2018 ◽

Vol 147 ◽

pp. 206-216 ◽

Cited By ~ 25

Author(s):

Seyed Majid Mousavi ◽

Babak Motesharezadeh ◽

Hossein Mirseyed Hosseini ◽

Hoseinali Alikhani ◽

Ali Asghar Zolfaghari

Keyword(s):

Plant Growth ◽

Contaminated Soil ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Induced Changes

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Remediation of Organically Contaminated Soil Through the Combination of Assisted Phytoremediation and Bioaugmentation

Applied Sciences ◽

10.3390/app9224757 ◽

2019 ◽

Vol 9 (22) ◽

pp. 4757 ◽

Cited By ~ 2

Author(s):

Mikel Anza ◽

Oihane Salazar ◽

Lur Epelde ◽

José María Becerril ◽

Itziar Alkorta ◽

...

Keyword(s):

Plant Growth ◽

Contaminated Soil ◽

Sodium Dodecyl ◽

Soil Health ◽

Organic Amendments ◽

Bacterial Consortium ◽

Soil Microbial Activity ◽

Total Petroleum Hydrocarbons ◽

Paenibacillus Sp ◽

Cow Slurry

Here, we aimed to bioremediate organically contaminated soil with Brassica napus and a bacterial consortium. The bioaugmentation consortium consisted of four endophyte strains that showed plant growth-promoting traits (three Pseudomonas and one Microbacterium) plus three strains with the capacity to degrade organic compounds (Burkholderia xenovorans LB400, Paenibacillus sp. and Lysinibacillus sp.). The organically contaminated soil was supplemented with rhamnolipid biosurfactant and sodium dodecyl benzenesulfonate to increase the degradability of the sorbed contaminants. Soils were treated with organic amendments (composted horse manure vs. dried cow slurry) to promote plant growth and stimulate soil microbial activity. Apart from quantification of the expected decrease in contaminant concentrations (total petroleum hydrocarbons, polycyclic aromatic hydrocarbons), the effectiveness of our approach was assessed in terms of the recovery of soil health, as reflected by the values of different microbial indicators of soil health. Although the applied treatments did not achieve a significant decrease in contaminant concentrations, a significant improvement of soil health was observed in our amended soils (especially in soils amended with dried cow slurry), pointing out a not-so-uncommon situation in which remediation efforts fail from the point of view of the reduction in contaminant concentrations while succeeding to recover soil health.

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Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17238859 ◽

2020 ◽

Vol 17 (23) ◽

pp. 8859 ◽

Cited By ~ 1

Author(s):

Muhammad Naveed ◽

Syeda Sosan Bukhari ◽

Adnan Mustafa ◽

Allah Ditta ◽

Saud Alamri ◽

...

Keyword(s):

Plant Growth ◽

Contaminated Soil ◽

Root Zone ◽

Plant Biomass ◽

Growth Promotion ◽

Research Work ◽

Control Treatment ◽

Soil Conditions ◽

Combined Application ◽

Bioavailable Fraction

Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.

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Effect of Cd-tolerant plant growth-promoting rhizobium on plant growth and Cd uptake by Lolium multiflorum Lam. and Glycine max (L.) Merr. in Cd-contaminated soil

Plant and Soil ◽

10.1007/s11104-013-1952-1 ◽

2013 ◽

Vol 375 (1-2) ◽

pp. 205-214 ◽

Cited By ~ 78

Author(s):

Junkang Guo ◽

Jie Chi

Keyword(s):

Glycine Max ◽

Plant Growth ◽

Contaminated Soil ◽

Lolium Multiflorum ◽

Cd Uptake ◽

Tolerant Plant ◽

Plant Growth Promoting ◽

Glycine Max L ◽

Growth Promoting

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Influence of plant growth promoting rhizobacterial strains Paenibacillus sp. IITISM08, Bacillus sp. PRB77 and Bacillus sp. PRB101 using Helianthus annuus on degradation of endosulfan from contaminated soil

Chemosphere ◽

10.1016/j.chemosphere.2019.03.037 ◽

2019 ◽

Vol 225 ◽

pp. 479-489 ◽

Cited By ~ 5

Author(s):

Rupa Rani ◽

Vipin Kumar ◽

Zeba Usmani ◽

Pratishtha Gupta ◽

Avantika Chandra

Keyword(s):

Plant Growth ◽

Helianthus Annuus ◽

Contaminated Soil ◽

Bacillus Sp ◽

Paenibacillus Sp ◽

Plant Growth Promoting ◽

Growth Promoting

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Effects of Soil Amendments on Heavy Metal Immobilization and Accumulation by Maize Grown in a Multiple-Metal-Contaminated Soil and Their Potential for Safe Crop Production

Toxics ◽

10.3390/toxics8040102 ◽

2020 ◽

Vol 8 (4) ◽

pp. 102

Author(s):

Fayuan Wang ◽

Shuqi Zhang ◽

Peng Cheng ◽

Shuwu Zhang ◽

Yuhuan Sun

Keyword(s):

Heavy Metal ◽

Plant Growth ◽

Contaminated Soil ◽

Crop Production ◽

Metal Uptake ◽

Soil Amendments ◽

Heavy Metal Uptake ◽

X Ray ◽

Metal Immobilization ◽

Heavy Metal Immobilization

Soil amendments have been proposed for immobilizing metallic contaminants, thus reducing their uptake by plants. For the safe production of crops in contaminated soil, there is a need to select suitable amendments that can mitigate heavy metal uptake and enhance crop yield. The present experiment compared the effects of three amendments, hydroxyapatite (HAP), organic manure (OM), and biochar (BC), on plant growth and heavy metal accumulation by maize in an acidic soil contaminated with Cd, Pb, and Zn, and their potential for safe crop production. Toxicity characteristic leaching procedure (TCLP) tests, energy dispersive X-ray spectroscopy (EDS) analysis, and X-ray diffraction (XRD) analysis were used to evaluate the effectiveness and mechanisms of heavy metal immobilization by the amendments. The results showed that shoot and root biomass was significantly increased by HAP and 1% OM, with an order of 1% HAP > 0.1% HAP > 1% OM, but not changed by 0.1% OM and BC (0.1% and 1%). HAP significantly decreased Cd, Pb, and Zn concentrations in both shoots and roots, and the effects were more pronounced at the higher doses. OM decreased the shoot Cd and Pb concentrations and root Zn concentrations, but only 1% OM decreased the shoot Zn and root Pb concentrations. BC decreased the shoot Cd and Pb concentrations, but decreased the shoot Zn and root Pb concentrations only at 1%. HAP decreased the translocation factors (TFs) of Cd, Pb, and Zn (except at the 0.1% dose). OM and BC decreased the TFs of Cd and Zn, respectively, at the 1% dose but showed no significant effects in other cases. Overall, plant P, K, Fe, and Cu nutrition was improved by HAP and 1% OM, but not by 0.1 OM and BC. Soil pH was significantly increased by HAP, 1% OM, and 1% BC, following an order of 1% HAP > 1% OM > 0.1% HAP > 1% BC. The TCLP levels for Cd, Pb, and Zn were significantly reduced by HAP, which can be partly attributed to its liming effects and the formation of sparingly soluble Cd-, Pb-, and Zn-P-containing minerals in the HAP-amended soils. To some extent, all the amendments positively influenced plant and soil traits, but HAP was the optimal one for stabilizing heavy metals, reducing heavy metal uptake, and promoting plant growth in the contaminated soil, suggesting its potential for safe crop production.

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Estimating Arsenic Mobility and Phytotoxicity Using Two Different Phosphorous Fertilizer Release Rates in Soil

Agronomy ◽

10.3390/agronomy9030111 ◽

2019 ◽

Vol 9 (3) ◽

pp. 111 ◽

Cited By ~ 1

Author(s):

Min-Suk Kim ◽

Hyun-Gi Min ◽

Jeong-Gyu Kim ◽

Sang-Ryong Lee

Keyword(s):

Acid Mine Drainage ◽

Plant Growth ◽

Contaminated Soil ◽

Mine Drainage ◽

Water Soluble ◽

Single Treatment ◽

Bone Meal ◽

Acid Mine ◽

Phytotoxicity Test ◽

P Fertilizer

Deficiencies in phosphorus (P), an essential factor for plant growth and aided phytostabilization, are commonly observed in soil, especially near mining areas. The objective of this study was to compare the effect of P-based fertilizer types on arsenic (As) extractability and phytotoxicity in As-contaminated soil after stabilizer treatment. Different treatments with respect to the P-releasing characteristics were applied to soil to determine As mobility and phytotoxicity in P-based fertilizers, with bone meal as a slow-releasing P fertilizer and fused superphosphate as a fast-releasing P fertilizer. In addition, P fertilizers were used to enhance plant growth, and two types of iron (Fe)-based stabilizers (steel slang and acid mine drainage sludge) were also used to reduce As mobility in As-contaminated soil under lab-scale conditions. A water-soluble extraction was conducted to determine As and P extractability. A phytotoxicity test using bok choy (Brassica campestris L. ssp. chinensis Jusl.) was performed to assess the elongation and accumulation of As and P. Within a single treatment, the As stabilization was higher in steel slag (84%) than in acid mine drainage sludge (27%), and the P supply effect was higher in fused superphosphate (24740%) than in bone meal (160%) compared to the control. However, a large dose of fused superphosphate (2%) increased not only the water-soluble P, but also the water-soluble As, and consequently, increased As uptake by bok choy roots, leading to phytotoxicity. In combined treatments, the tendency towards change was similar to that of the single treatment, but the degree of change was decreased compared to the single treatment, thereby decreasing the risk of phytotoxicity. In particular, the toxicity observed in the fused superphosphate treatments did not appear in the bone meal treatment, but rather the growth enhancement effect appeared. These results indicate that the simultaneous application of bone meal and stabilizers might be proposed and could effectively increase plant growth via the stabilization of As and supplementation with P over the long term.

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