Enteric Parasites

Abstract Background Blastocystis sp. is one of the most common enteric parasites of humans and animals worldwide. It is well recognized that this ubiquitous protist displays a remarkable degree of genetic diversity in the SSU rRNA gene, which is currently the main gene used for defining Blastocystis subtypes. Yet, full-length reference sequences of this gene are available for only 16 subtypes of Blastocystis in part because of the technical difficulties associated with obtaining these sequences from complex samples. Methods We have developed a method using Oxford Nanopore MinION long-read sequencing and universal eukaryotic primers to produce full-length (> 1800 bp) SSU rRNA gene sequences for Blastocystis. Seven Blastocystis specimens representing five subtypes (ST1, ST4, ST10, ST11, and ST14) obtained both from cultures and feces were used for validation. Results We demonstrate that this method can be used to produce highly accurate full-length sequences from both cultured and fecal DNA isolates. Full-length sequences were successfully obtained from all five subtypes including ST11 for which no full-length reference sequence currently exists and for an isolate that contained mixed ST10/ST14. Conclusions The suitability of the use of MinION long-read sequencing technology to successfully generate full-length Blastocystis SSU rRNA gene sequences was demonstrated. The ability to produce full-length SSU rRNA gene sequences is key in understanding the role of genetic diversity in important aspects of Blastocystis biology such as transmission, host specificity, and pathogenicity.

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A STUDY OF STOOL SAMPLE FROM HIV POSITIVE AND HIV NEGATIVE AT ANDHRA PRADESH.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i1.19377 ◽

2018 ◽

Vol 11 (1) ◽

pp. 394

Author(s):

Gundala Obulesu ◽

Hanumanthappa Ar ◽

Prabakar Reddy E

Keyword(s):

Andhra Pradesh ◽

Intestinal Parasites ◽

Parasitic Infection ◽

Hiv Positive ◽

Hiv Status ◽

Concentration Technique ◽

Wet Mount ◽

Positive Person ◽

Enteric Parasites ◽

Hiv Negative

Objective: The objective of this study is to investigate the variations, if any, in the types of intestinal parasites in HIV-positive and HIV-negative individuals.Methods: For their HIV status in our laboratory by enzyme-linked immunoasorbant assay and TRIDOT, sample stool, macroscopic and microscopic examination, formal-ether concentration technique f, saline wet mount, iodine wet mount, modified acid-fast stain. Results: For the purpose of statistical analysis, the enteric parasites are divided into the coccidian group (Cryptosporidium+Isospora belli) and conventional enteric parasites (Entamoeba histolytica, Giardia intestinalis, and Ascaris lumbricoides), and enteric parasitic infection is more common in HIV-positive patients with diarrhea than HIV-positive patient without diarrhea or HIV-negative individuals.Conclusion: The study period of 1 year and 8 months revealed that it is difficult to locate/identify a HIV-positive person in a place like Kadapa on account of confidentiality/lack of awareness among the population. Detection of intestinal parasites is 17.3% in HIV-positive patients and 12% in HIV-negative individuals.

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Nosocomial Transmission and Infection Control Aspects of Parasitic and Ectoparasitic Diseases: Part I. Introduction/Enteric Parasites

Infection Control and Hospital Epidemiology ◽

10.1086/646239 ◽

1991 ◽

Vol 12 (01) ◽

pp. 59-66

Author(s):

Ludwig A. Lettau

Keyword(s):

Infection Control ◽

Nosocomial Transmission ◽

Enteric Parasites

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