Full-Length Spine CT and MRI in Daily Practice

Background In the diagnosis of bone and soft-tissue sarcomas, the continuous advancement of various imaging modalities has improved the detection of small lesions, surgical planning, assessment of chemotherapeutic effects, and, importantly, guidance for surgery or biopsy. Method This review was composed based on a PubMed literature search for the terms “bone sarcoma,” “bone cancer” and “soft tissue sarcoma,” “imaging,” “magnetic resonance imaging”, “computed tomography”, “ultrasound”, “radiography”, and “radiomics” covering the publication period 2005–2020. Results and Conclusion As discussed in this review, radiography, ultrasound, CT, and MRI all play key roles in the imaging evaluation of bone and soft-tissue sarcomas. In daily practice, advanced MRI techniques complement standard MRI but remain underused, as they are considered time-consuming, technically challenging, and not reliable enough to replace biopsy and histology. PET/MRI and radiomics have shown promise regarding the imaging of sarcomas in the future. Key Points: Citation Format

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Emphysematous Osteomyelitis of the Spine - CT and MRI Appearance and Associated Artifact

10.26226/morressier.5e8335ba7cb08a046ef7c7f9 ◽

2020 ◽

Author(s):

Zerwa Farooq

Keyword(s):

Spine Ct ◽

Ct And Mri

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Cervical Spine CT and MRI Findings in a Patient with Ossification of the Posterior Longitudinal Ligament

Journal of the Korean Neurological Association ◽

10.17340/jkna.2015.1.19 ◽

2015 ◽

Vol 33 (1) ◽

pp. 67-68

Author(s):

Sang Bub Lee ◽

Chang Beom Bae ◽

Dong Kuck Lee

Keyword(s):

Cervical Spine ◽

Posterior Longitudinal Ligament ◽

Mri Findings ◽

Spine Ct ◽

Ct And Mri

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Lumbar Spine CT and MRI

Radiology ◽

10.1148/radiology.186.1.226 ◽

1993 ◽

Vol 186 (1) ◽

pp. 226-226

Author(s):

Jannice O. Aaron

Keyword(s):

Lumbar Spine ◽

Spine Ct ◽

Ct And Mri

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Synovial cysts of the lumbar spine: CT and MRI correlations

European Radiology ◽

10.1007/bf00599066 ◽

1994 ◽

Vol 4 (4) ◽

pp. 332-336 ◽

Cited By ~ 1

Author(s):

P. Reginster ◽

J. Collignon ◽

R. F. Dondelinger

Keyword(s):

Lumbar Spine ◽

Spine Ct ◽

Synovial Cysts ◽

Ct And Mri

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Trauma of the spine: CT and MRI

European Journal of Radiology ◽

10.1016/0720-048x(91)90141-h ◽

1991 ◽

Vol 12 (1) ◽

pp. 79-80

Author(s):

J.T. Wilmink

Keyword(s):

Spine Ct ◽

Ct And Mri

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Gorham syndrome of the thorax and cervical spine: CT and MRI findings

Skeletal Radiology ◽

10.1007/s002560050192 ◽

1997 ◽

Vol 26 (1) ◽

pp. 55-59 ◽

Cited By ~ 26

Author(s):

Christine Chung ◽

Joseph S. Yu ◽

Donald Resnick ◽

Luke M. Vaughan ◽

Parvis Haghighi

Keyword(s):

Cervical Spine ◽

Mri Findings ◽

Spine Ct ◽

Ct And Mri

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Lumbar spine CT and MRI

Pediatric Radiology ◽

10.1007/bf02017673 ◽

1994 ◽

Vol 24 (1) ◽

pp. 75-75

Author(s):

T. Jaspan

Keyword(s):

Lumbar Spine ◽

Spine Ct ◽

Ct And Mri

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In Situ Localization of PPAR Gamma and Uncoupling Protein in Mouse Embryo Sections Using Digoxigenin-Labeled Riboprobes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162624 ◽

1996 ◽

Vol 54 ◽

pp. 32-33

Author(s):

C. Jennermann ◽

S. A. Kliewer ◽

D. C. Morris

Keyword(s):

Alkaline Phosphatase ◽

Room Temperature ◽

Uncoupling Protein ◽

Ppar Gamma ◽

Nuclear Hormone Receptor ◽

Full Length ◽

Northern Analysis ◽

Peroxisome Proliferator

Peroxisome proliferator-activated receptor gamma (PPARg) is a member of the nuclear hormone receptor superfamily and has been shown in vitro to regulate genes involved in lipid metabolism and adipocyte differentiation. By Northern analysis, we and other researchers have shown that expression of this receptor predominates in adipose tissue in adult mice, and appears first in whole-embryo mRNA at 13.5 days postconception. In situ hybridization was used to find out in which developing tissues PPARg is specifically expressed.Digoxigenin-labeled riboprobes were generated using the Genius™ 4 RNA Labeling Kit from Boehringer Mannheim. Full length PPAR gamma, obtained by PCR from mouse liver cDNA, was inserted into pBluescript SK and used as template for the transcription reaction. Probes of average size 200 base pairs were made by partial alkaline hydrolysis of the full length transcripts. The in situ hybridization assays were performed as described previously with some modifications. Frozen sections (10 μm thick) of day 18 mouse embryos were cut, fixed with 4% paraformaldehyde and acetylated with 0.25% acetic anhydride in 1.0M triethanolamine buffer. The sections were incubated for 2 hours at room temperature in pre-hybridization buffer, and were then hybridized with a probe concentration of 200μg per ml at 70° C, overnight in a humidified chamber. Following stringent washes in SSC buffers, the immunological detection steps were performed at room temperature. The alkaline phosphatase labeled, anti-digoxigenin antibody and detection buffers were purchased from Boehringer Mannheim. The sections were treated with a blocking buffer for one hour and incubated with antibody solution at a 1:5000 dilution for 2 hours, both at room temperature. Colored precipitate was formed by exposure to the alkaline phosphatase substrate nitrobluetetrazoliumchloride/ bromo-chloroindlylphosphate.

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