Rapid determination of antimicrobial susceptibility by SRS single-cell metabolic imaging

2022 ◽  
pp. 445-461
Author(s):  
Weili Hong ◽  
Meng Zhang ◽  
Ji-Xin Cheng
1973 ◽  
Vol 59 (5) ◽  
pp. 693-699 ◽  
Author(s):  
A. L. Barry ◽  
L. J. Joyce ◽  
A. P. Adams ◽  
E. J. Benner

Lab on a Chip ◽  
2014 ◽  
Vol 14 (22) ◽  
pp. 4426-4434 ◽  
Author(s):  
Yuliang Zhao ◽  
Hok Sum Sam Lai ◽  
Guanglie Zhang ◽  
Gwo-Bin Lee ◽  
Wen Jung Li

Single-cell density and mass were measured rapidly using a combination of optically induced electrokinetics, microfluidics, computer vision, and classical sedimentation theory.


Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 288
Author(s):  
Britney Forsyth ◽  
Peter Torab ◽  
Jyong-Huei Lee ◽  
Tyler Malcom ◽  
Tza-Huei Wang ◽  
...  

Bloodstream infections are a significant cause of morbidity and mortality worldwide. The rapid initiation of effective antibiotic treatment is critical for patients with bloodstream infections. However, the diagnosis of bloodborne pathogens is largely complicated by the matrix effect of blood and the lengthy blood tube culture procedure. Here we report a culture-free workflow for the rapid isolation and enrichment of bacterial pathogens from whole blood for single-cell antimicrobial susceptibility testing (AST). A dextran sedimentation step reduces the concentration of blood cells by 4 orders of magnitude in 20–30 min while maintaining the effective concentration of bacteria in the sample. Red blood cell depletion facilitates the downstream centrifugation-based enrichment step at a sepsis-relevant bacteria concentration. The workflow is compatible with common antibiotic-resistant bacteria and does not influence the minimum inhibitory concentrations. By applying a microfluidic single-cell trapping device, we demonstrate the workflow for the rapid determination of bacterial infection and antimicrobial susceptibility testing at the single-cell level. The entire workflow from blood to categorical AST result can be completed in less than two hours.


Author(s):  
T. Y. Tan ◽  
W. K. Tice

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.


2017 ◽  
Vol 45 (2) ◽  
pp. 455-464
Author(s):  
T.T. Xue ◽  
J. Liu ◽  
Y.B. Shen ◽  
G.Q. Liu

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