Rapid determination of antimicrobial susceptibility patterns ofListeria monocytogenes isolates by the autobac AIS and MIC procedures

1987 ◽  
Vol 15 (3) ◽  
pp. 137-140
Author(s):  
Patrick A. Dillon ◽  
Sue B. Overman ◽  
Timothy L. Overman
Keyword(s):  
Antimicrobial Susceptibility ◽  
Rapid Determination ◽  
Oflisteria Monocytogenes ◽  
Susceptibility Patterns

Rapid Determination of Antimicrobial Susceptibility for Urgent Clinical Situations

1973 ◽  
Vol 59 (5) ◽  
pp. 693-699 ◽  
Author(s):  
A. L. Barry ◽  
L. J. Joyce ◽  
A. P. Adams ◽  
E. J. Benner
Keyword(s):  
Antimicrobial Susceptibility ◽  
Rapid Determination

scholarly journals Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas

2019 ◽  
Author(s):  
Nima Bahador ◽  
Saeed Shoja ◽  
Foroogh Faridi ◽  
Banafsheh Dozandeh-Mobarrez ◽  
Fatemeh Izadpanah Qeshmi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Antimicrobial Susceptibility ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Clinical Specimens ◽  
Biofilm Production ◽  
Bandar Abbas ◽  
Susceptibility Patterns

Background and Objectives: Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen. The presence of several virulence factors such as exotoxin and exoenzyme genes and biofilm may contribute to its pathogenicity. The purpose of this study was to investigate the presence of toxA, exoU and exoS, the determination of biofilm production and antimicrobial susceptibility patterns among clinical isolates of P. aeruginosa. Materials and Methods: In this study, 75 isolates of P. aeruginosa were recovered from various clinical specimens. Antimi- crobial susceptibility pattern of isolates were identified. Virulence genes toxA, exoU and exoS were determined using PCR. The ability of biofilm production was assessed. Results: Antimicrobial susceptibility test showed that 12 strains were resistant to more than 8 antibiotics (17.14%). The most effective antibiotic was colistin as 98.6% of isolates were sensitive. The frequencies of exoU and exoS genes were detected as 36.6% and 55.7%, respectively. In addition, 98.6% of the isolates were biofilm producers. Exotoxin A was detected in sixty-eight isolates (95.7%). Conclusion: The findings of this study showed that, the presence of P. aeruginosa exotoxin and exoenzyme genes, particu- larly, the exoU gene is the most common virulence factors in the bacterial isolates from urine samples. Biofilm is a serious challenge in the treatment of P. aeruginosa infection.

scholarly journals Antimicrobial Resistant Streptococcus pneumoniae

2010 ◽  
Vol 49 (179) ◽  
Author(s):  
B Khanal ◽  
A Acharya ◽  
R Amatya ◽  
R Gurung ◽  
N Paudyal ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Pneumococcal Infections ◽  
Intermediate Resistance ◽  
Resistance To Penicillin ◽  
Susceptibility Patterns

Introduction: Pneumococcal infections are important cause of morbidity and mortality. Knowledge of antimicrobial susceptibility patterns plays important role in the selection of appropriate therapy.  Present study was undertaken to analyze the susceptibility patterns of pneumococcal isolates against  commonly used antimicrobials with special reference to determination of minimum inhibitory  concentration (MIC) of penicillin in a tertiary care hospital in eastern Nepal. Methods: Twenty-six strains of S. pneumoniae isolated from various clinical specimens submitted to microbiology laboratory were evaluated. All isolates were tested for antimicrobial susceptibility by  disk diffusion method. MIC of penicillin was tested by broth dilution method. Results: Of the total isolates 19 (73%) were from invasive infections. Seven isolates were resistant to cotrimoxazole. No resistance to penicillin was seen in disk diffusion testing. Less susceptibility to penicillin (MIC 0.1-1.0 mg/L) was observed in five (17%) isolates. High level resistance to penicillin was not detected. One isolate was multidrug resistant. Conclusions: S. pneumoniaeisolates with intermediate resistance to penicillin prevail in Tertiary Care Hospital in eastern Nepal, causing invasive and noninvasive infections. As intermediate resistance is not detected in routine susceptibility testing, determination of MIC is important. It helps not only in the effective management of life threatening infections but is also essential in continuous monitoring and early detection of resistance. In addition, further study on pneumococcal infections, its antimicrobial resistance profile and correlation with clinical and epidemiological features including serotypes and group prevalence is recommended in future. Keywords: antimicrobial susceptibility pattern, penicillin, Streptococcus pneumoniae.

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