Approach for defining endogenous reference genes in gene expression experiments

2004 ◽  
Vol 329 (2) ◽  
pp. 293-299 ◽  
Author(s):  
J.J Garcı́a-Vallejo ◽  
B Van het Hof ◽  
J Robben ◽  
J.A.E Van Wijk ◽  
I Van Die ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

Gene Expression Analysis in Superficial Bladder Cancer: Comparison of Two Suitable Endogenous Reference Genes

2007 ◽  
Vol 1 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Paula A. Videira ◽  
Dário Ligeiro ◽  
Manuela Correia ◽  
Hélder Trindade
Keyword(s):  
Gene Expression ◽  
Bladder Cancer ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Superficial Bladder Cancer ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

Selection of endogenous reference genes for gene expression analysis in Leishmania major developmental stages

2007 ◽  
Vol 101 (2) ◽  
pp. 473-477 ◽  
Author(s):  
Meriam Ouakad ◽  
Narges Bahi-Jaber ◽  
Mehdi Chenik ◽  
Koussay Dellagi ◽  
Hechmi Louzir
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Leishmania Major ◽  
Developmental Stages ◽  
Endogenous Reference ◽  
Selection Of ◽  
Endogenous Reference Genes

scholarly journals Validation of endogenous reference genes in rat cerebral cortex for RT-qPCR analyses in developmental toxicity studies

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7181 ◽  
Author(s):  
Louise Ramhøj ◽  
Marta Axelstad ◽  
Terje Svingen
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Reference Genes ◽  
In Utero ◽  
Rat Cerebral Cortex ◽  
In Utero Exposure ◽  
Relative Gene Expression ◽  
Endogenous Reference ◽  
Relative Gene ◽  
Endogenous Reference Genes

Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must be stably expressed across all the tested samples. Here, we have tested 10 common RGs for their expression stability in cerebral cortex from young rats after in utero exposure to thyroid hormone (TH) disrupting compounds. We found that all 10 RGs were stable according to the three algorithms geNorm, NormFinder and BestKeeper. The downstream target gene Pvalb was significantly downregulated in brains from young rats after in utero exposure to propylthiouracil (PTU), a medicinal drug inhibiting TH synthesis. Similar results were obtained regardless of which of the 10 RGs was used for normalization. Another potential gene affected by developmental TH disruption, Dio2, was either not affected, or significantly upregulated about 1.4-fold, depending on which RG was used for normalization. This highlights the importance of carefully selecting correct RGs for normalization and to take into account the sensitivity of the RT-qPCR method when reporting on changes to gene expression that are less than 1.5-fold. For future studies examining relative gene expression in rat cerebral cortex under toxicological conditions, we recommend using a combination of either Rps18/Rpl13a or Rps18/Ubc for normalization, but also continuously monitor any potential regulation of the RGs themselves following alterations to study protocols.

scholarly journals ACTB and SDHA Are Suitable Endogenous Reference Genes for Gene Expression Studies in Human Astrocytomas Using Quantitative RT-PCR

2018 ◽  
Vol 17 ◽  
pp. 153303381880231 ◽  
Author(s):  
Gabriele Röhn ◽  
Arend Koch ◽  
Boris Krischek ◽  
Pantelis Stavrinou ◽  
Roland Goldbrunner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Rt Pcr ◽  
Expression Studies ◽  
Endogenous Reference ◽  
Gene Expression Studies ◽  
Human Astrocytomas ◽  
Endogenous Reference Genes

scholarly journals Endogenous Reference Genes for Gene Expression Studies on Bicuspid Aortic Valve Associated Aortopathy in Humans

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164329 ◽  
Author(s):  
Oliver J. Harrison ◽  
Narain Moorjani ◽  
Christopher Torrens ◽  
Sunil K. Ohri ◽  
Felino R. Cagampang
Keyword(s):  
Gene Expression ◽  
Aortic Valve ◽  
Bicuspid Aortic Valve ◽  
Reference Genes ◽  
Expression Studies ◽  
Endogenous Reference ◽  
Gene Expression Studies ◽  
Endogenous Reference Genes

Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration

2010 ◽  
Vol 38 (7) ◽  
pp. 4423-4428 ◽  
Author(s):  
Yan-qing Yuwen ◽  
Zi-mei Dong ◽  
Qing-hua Wang ◽  
Xiao-juan Sun ◽  
Chang-ying Shi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Rt Pcr ◽  
Planarian Regeneration ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

scholarly journals A new set of endogenous reference genes for gene expression studies of porcine stomach

2010 ◽  
Vol 19 (4) ◽  
pp. 570-576 ◽  
Author(s):  
M. Oczkowicz ◽  
M. Różycki ◽  
K. Piórkowska ◽  
A. Piestrzyńska-Kajtoch ◽  
B. Rejduch
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Expression Studies ◽  
Endogenous Reference ◽  
Gene Expression Studies ◽  
Endogenous Reference Genes
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