Highlighting the tricarboxylic acid cycle: Liquid and gas chromatography–mass spectrometry analyses of 13C-labeled organic acids

2013 ◽  
Vol 436 (2) ◽  
pp. 151-159 ◽  
Author(s):  
Mohamed Koubaa ◽  
Jean-Christophe Cocuron ◽  
Brigitte Thomasset ◽  
Ana Paula Alonso
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Acid Cycle ◽  
Chromatography Mass Spectrometry

scholarly journals Compartmentation of acetyl CoA studied by analysis of tricarboxylic acid cycle acids and 3-hydroxybutyrate in bile of rats given [2,2,2-2H3]ethanol

1990 ◽  
Vol 265 (2) ◽  
pp. 569-574 ◽  
Author(s):  
C Norsten ◽  
T Cronholm
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ketone Bodies ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Acetyl Coa ◽  
Acid Cycle ◽  
Tricarboxylic Acid Cycle Intermediates ◽  
Rat Bile

Acetate, 3-hydroxybutyrate, pyruvate, lactate, citrate, 2-oxoglutarate, succinate, fumarate and malate were analysed in rat bile by gas chromatography and gas chromatography/mass spectrometry of their O-melthyloxime-t-butyldimethylsilyl derivatives. The concentration of acetate increased to about 1.8 mmol/l after administration of [2,2,2-2H3]ethanol. Acetate was formed from ethanol to an extent of about 82% and retained all of the 2H at C-2, whereas 15% of the 2H had been lost in the tricarboxylic acid cycle intermediates and 24% in 3-hydroxybutyrate. Thus the exchange of 2H for 1H takes place after formation of acetyl CoA. For citrate and 3-hydroxybutyrate, 41% and 11% respectively was formed from [2,2,2-2H3]ethanol. These results indicate that different pools of acetyl CoA are used for the synthesis of ketone bodies and citrate, with the latter being derived from ethanol to a much larger extent. Smaller fractions of 2-oxoglutarate (16%) and succinate (5%) were derived from [2,2,2--2H3]ethanol, indicating significant contributions from amino acids.

scholarly journals Comparison of organic acids in three parts of the human heart by gas chromatography-mass spectrometry.

1982 ◽  
Vol 29 (1) ◽  
pp. 49-53
Author(s):  
SHIN-ICHI HARAGUCHI ◽  
HIRONORI TOSHIMA ◽  
MICHIHIRO KOGA ◽  
KIROKU OHISHI ◽  
HIROMI TAKAGI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Human Heart ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Metabolic profiling of organic acids in urine samples of Cri Du Chat syndrome individuals by gas chromatography-mass spectrometry

2020 ◽  
Vol 1153 ◽  
pp. 122267
Author(s):  
Bruno Rafael Araújo ◽  
Danielle Zildeana Sousa Furtado ◽  
Fernando Brunale Vilela de Moura Leite ◽  
Nilson Antonio de Assunção ◽  
Emanuel Carrilho
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Metabolic Profiling ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Cri Du Chat Syndrome ◽  
Chromatography Mass Spectrometry
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