A mass spectrometry-based approach to host cell protein identification and its application in a comparability exercise

2014 ◽  
Vol 463 ◽  
pp. 1-6 ◽  
Author(s):  
Veronika Reisinger ◽  
Hansjoerg Toll ◽  
Robert Ernst Mayer ◽  
Jan Visser ◽  
Florian Wolschin
Keyword(s):  
Mass Spectrometry ◽  
Host Cell ◽  
Protein Identification ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Comparability Exercise

scholarly journals Mass spectrometry analysis reveals differences in the host cell protein species found in pseudotyped lentiviral vectors

Biologicals ◽  
2018 ◽  
Vol 52 ◽  
pp. 59-66 ◽  
Author(s):  
Sabine Johnson ◽  
Jun X. Wheeler ◽  
Robin Thorpe ◽  
Mary Collins ◽  
Yasuhiro Takeuchi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Cell ◽  
Lentiviral Vectors ◽  
Mass Spectrometry Analysis ◽  
Cell Protein ◽  
Protein Species ◽  
Host Cell Protein ◽  
Spectrometry Analysis

scholarly journals A modular and adaptive mass spectrometry-based platform for support of bioprocess development toward optimal host cell protein clearance

mAbs ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 654-663 ◽  
Author(s):  
Donald E. Walker ◽  
Feng Yang ◽  
Joseph Carver ◽  
Koman Joe ◽  
David A. Michels ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Cell ◽  
Cell Protein ◽  
Bioprocess Development ◽  
Host Cell Protein ◽  
Protein Clearance

Biopharmaceutical quality control with mass spectrometry

Bioanalysis ◽  
2021 ◽  
Author(s):  
Shulei Liu ◽  
Benjamin L Schulz
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Protein Identification ◽  
Structural Integrity ◽  
Cell Protein ◽  
Manufacturing Processes ◽  
Host Cell Protein ◽  
Post Translational Modifications ◽  
Biochemical Studies

Mass spectrometry (MS) is a powerful technique for protein identification, quantification and characterization that is widely applied in biochemical studies, and which can provide data on the quantity, structural integrity and post-translational modifications of proteins. It is therefore a versatile and widely used analytic tool for quality control of biopharmaceuticals, especially in quantifying host-cell protein impurities, identifying post-translation modifications and structural characterization of biopharmaceutical proteins. Here, we summarize recent advances in MS-based analyses of these key quality attributes of the biopharmaceutical development and manufacturing processes.

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics

2016 ◽  
Vol 18 (6) ◽  
pp. 1439-1452 ◽  
Author(s):  
Vibha Jawa ◽  
Marisa K. Joubert ◽  
Qingchun Zhang ◽  
Meghana Deshpande ◽  
Suminda Hapuarachchi ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Nadine Kochanowski ◽  
Gaetan Siriez ◽  
Larissa Mukankurayija ◽  
Aurélie Delangle ◽  
Alex Murray-Smith ◽  
...  
Keyword(s):  
Host Cell ◽  
Mammalian Cells ◽  
Assay Development ◽  
Cell Protein ◽  
Host Cell Protein
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