Validation of a two-plate microbiological method for screening antibiotic residues in shrimp tissue

Urinary tract infection caused by Enterococci has become frequent occurrences in health care settings. Currently they emerged with increasing resistance to multiple antibiotics. Haemolysin, gelatinase and biofilm production are some markers that have been proposed as possible Enterococcal virulence factors. In view of the increasing importance of Enterococcal infection, the present study was designed to isolate and identify the Enterococci to the species level from urine of urinary tract infection patients and to investigate their possible virulence factors. Biofilm was detected on polystyrene microtitre plate to see the adherence of microorganism. Haemolysin production and gelatin hydrolysis detected by standard microbiological method. Fifty nine enterococcal isolates were speciated by conventional microbiological method and examined for their ability to form biofilm by microtitre plate assay. In this study, biofilm formations by Enterococci were found in 83.33% isolates from catheterized and 56.09% from non-catheterized patients. Aong them, E.faecalis & 50% E.faecium produced biofilm. About 43.63% E.faecalis & 10% E.faecium produced haemolysin and only one isolate were found to be gelatinase positive. Frequency of virulence factors (VFs) in combination was observed in this study. Two VFs (haemolysin and biofilm) were observed in 27.11% in combination and 3 VFs ( haemolysinm biofilm and gelatinase) were present in 1.69% isolates. These results suggest that although there may not be an absolute role for individual virulence determinants in infectivity, combinations of factors may play a role in allowing a biofilm infection to be more resistant to therapy.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19361 Bangladesh J Med Microbiol 2012; 06(01): 14-17

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Microbiological Method for Eor

Revue de l Institut Français du Pétrole ◽

10.2516/ogst:1990010 ◽

1990 ◽

Vol 45 (1) ◽

pp. 115-121 ◽

Cited By ~ 4

Author(s):

E. M. Yulbarisov

Keyword(s):

Microbiological Method

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Determination of 50 antibiotic residues in drone pupa powder by liquid chromatography-tandem mass spectrometry

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2018.08046 ◽

2019 ◽

Vol 37 (1) ◽

pp. 46 ◽

Cited By ~ 1

Author(s):

Wenquan YANG ◽

Xiaoyan ZHANG ◽

Qian YAO ◽

Yangjuan ZHOU ◽

Yawen HU ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Tandem Mass ◽

Antibiotic Residues ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

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Evaluate antibiotic residues in beef and effect of cooking and freezing on it.

Benha Veterinary Medical Journal ◽

10.21608/bvmj.2019.13927.1029 ◽

2019 ◽

Vol 36 (2) ◽

pp. 109-116

Author(s):

Heba Fahim ◽

Fahim shaltout ◽

Mohamed A. El shatter

Keyword(s):

Antibiotic Residues

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Advances in standardizing and validating research techniques for pathogen monitoring in water

Water Science & Technology Water Supply ◽

10.2166/ws.2004.0023 ◽

2004 ◽

Vol 4 (2) ◽

pp. 23-30

Author(s):

K. Connell ◽

M. Pope ◽

K. Miller ◽

J. Scheller ◽

J. Pulz

Keyword(s):

Validation Studies ◽

Development Process ◽

Monitoring Methods ◽

Microbiological Method ◽

Validation Process ◽

Method Performance ◽

Target Analytes ◽

Interlaboratory Validation ◽

Quality Control Criteria ◽

Control Criteria

Designing and conducting standardized microbiological method interlaboratory validation studies is challenging because most methods are manual, rather than instrument-based, and results from the methods are typically subjective. Determinations of method recovery, in particular, are problematic, due to difficulties in assessing the true spike amount. The standardization and validation process used for the seven most recent USEPA 1600-series pathogen monitoring methods has begun to address these challenges. A staged development process was used to ensure that methods were adequately tested and standardized before resources were dedicated to interlaboratory validation. The interlaboratory validation studies for USEPA Method 1622, for Cryptosporidium, USEPA Method 1601 for coliphage, and USEPA Method 1605 for Aeromonas assessed method performance using different approaches, due the differences in the nature of the target analytes and the data quality needs of each study. However, the use of enumerated spikes in all of the studies allowed method recovery and precision to be assessed, and also provided the data needed to establish quantitative quality control criteria for the methods.

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