In situ imaging and interfering Dicer-mediated cleavage process via a versatile molecular beacon probe

2019 ◽  
Vol 1079 ◽  
pp. 146-152 ◽  
Author(s):  
Kai Zhang ◽  
Xue-Jiao Yang ◽  
Ting-Ting Zhang ◽  
Xiang-Ling Li ◽  
Hong-Yuan Chen ◽  
...  
2020 ◽  
Vol 12 (18) ◽  
pp. 2385-2390
Author(s):  
Tao Xie ◽  
Ziyan Fan ◽  
Ruilong Zhang ◽  
Xiaohe Tian ◽  
Guangmei Han ◽  
...  

We develop molecular beacon-functionalized gold nanoparticles for in situ human telomerase RNA imaging in live cells.


2011 ◽  
Vol 131 (12) ◽  
pp. 427-428
Author(s):  
Toshihiko Noda ◽  
Pan Yi-Li ◽  
Ayato Tagawa ◽  
Takuma Kobayashi ◽  
Kiyotaka Sasagawa ◽  
...  

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.


2021 ◽  
Author(s):  
Rongxin Fu ◽  
Ya Su ◽  
Ruliang Wang ◽  
Xue Lin ◽  
Xiangyu Jin ◽  
...  

2014 ◽  
Vol 145 ◽  
pp. 838-842 ◽  
Author(s):  
Meng Sun ◽  
Zhan-Jun Li ◽  
Chun-Lin Liu ◽  
Hai-Xia Fu ◽  
Jiang-Shan Shen ◽  
...  

2009 ◽  
Vol 14 (2) ◽  
pp. 024024 ◽  
Author(s):  
Eyal Bar-Kochba ◽  
Saagar Govil ◽  
Jon P. Longtin ◽  
Andrew Gouldstone ◽  
Mary D. Frame

2002 ◽  
Vol 41 (3P2) ◽  
pp. 551-554 ◽  
Author(s):  
Charles A. Gentile ◽  
John J. Parker ◽  
Stewart J. Zweben

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