Wavelength selection of the multispectral lidar system for estimating leaf chlorophyll and water contents through the PROSPECT model

2019 ◽  
Vol 266-267 ◽  
pp. 43-52 ◽  
Author(s):  
Jia Sun ◽  
Shuo Shi ◽  
Jian Yang ◽  
Wei Gong ◽  
Feng Qiu ◽  
...  
1998 ◽  
Vol 4 (S2) ◽  
pp. 126-127
Author(s):  
K. R. Spring

Many recent applications in light microscopy involve the use of multiple fluorophores or the delineation of signals arising from spectrally distinct sources. In microspectroscopy, it is always desirable to illuminate fluorescently-labeled microscopic specimens with monochromatic light as the narrowest possible excitation wavelength range usually results in the highest emission signal-to-noise ratio. Generation of polychromatic light from an arc lamp and selection of the excitation wavelength by interference filters or monochrometers are the most common techniques for excitation microspectrofluorometry. Emission spectroscopy is usually done with filter wheels, monochrometers, or interferometers inserted between the microscope detection port and the detector. This presentation will be directed toward other, less frequently-used, approaches for spectral scanning of the specimen in the light microscope. Three topics will be considered: 1) the use of acousto-optical tunable filters and lasers for rapid, narrow-band, excitation wavelength selection; 2) the use of holographic notch filters for rejection of unwanted excitation laser light; 3) using liquid-crystal tunable filters for emission scanning.


2008 ◽  
Vol 62 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Nguyen The Quyen ◽  
Michel D. Jouan ◽  
Nguyen Quy Dao ◽  
Edouard Da Silva ◽  
Duong Ai Phuong

In this paper, the performances of four improved analytical methods (backward stepwise selection of peak intensities, sum of characteristic peaks of a component, moving window partial least squares, and genetic algorithms) using wavelength selection for the analysis of xylene mixtures by Raman spectroscopy are tested for further use on the new “digital micromirror device associated with a photomultiplier tube” Raman spectrometer. It is shown that the errors of prediction using only a few selected points (from 4 to 49 depending on the method) are almost the same as when using the whole spectral range (1050 points). Compared to the last two methods, the “backward stepwise selection of peak intensities” and “sum of characteristic peaks of a component” methods are robust under industrial conditions and appear to be well suited for chemical quantitative analysis with the new Raman spectrometer, which allows the measurements of the total intensity to be made simultaneously for a number of pre-selected frequencies. Results show that the errors of prediction can be near to or even lower than 2%.


2014 ◽  
Vol 34 (9) ◽  
pp. 0930002
Author(s):  
余晓娅 Yu Xiaoya ◽  
张玉钧 Zhang Yujun ◽  
殷高方 Yin Gaofang ◽  
肖雪 Xiao Xue ◽  
赵南京 Zhao Nanjing ◽  
...  

METANA ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 13
Author(s):  
Kartika Dian Elliana ◽  
R.T.D. Wisnu Broto

Ekstraksi merupakan proses pemisahan dan isolasi zat dari suatu zat dengan penambahan pelarut tertentu untuk mengeluarkan komponen campuran dari zat padat atau zat cair. Pada penelitian ini dilakukan ekstraksi klorofilid dari daun mangga untuk mengetahui stabilitas reduksi klorofil hasil ekstraksi berupa ekstrak dan rafinat. Ekstraksi klorofilid dilakukan dengan alat ekstraktor berpengaduk yaitu dengan memotong daun mangga 1 cm kemudian di ekstraksi dengan pelarut Aquadest 25%:75% Isopropil Alkohol.  Pemilihan pelarut merupakan faktor yang menentukan dalam proses ekstraksi. Hal-hal yang perlu diperhatikan dalam pemilihan pelarut adalah selektivitas, sifat pelarut, kemampuan untuk mengekstraksi, tidak bersifat racun, mudah diuapkan dan harganya relatif murah. Karakteristik ekstrak klorofilid menggunakan spektrofotometer untuk mengetahui absorbansi klorofil optimum dalam suatu variabel ekstrak dan rafinat serta uji pH untuk mengetahui stabilitas pH yang baik untuk mereduksi klorofil daun mangga. Hasil absorbansi klorofil optimum pada suhu 60OC dan waktu 70 menit pada ekstrak dan hasil stabilitas pH untuk mereduksi klorofil didapatkan pada pH 9 asam serta pH 2 basa.Extraction is the process of separating and isolating substances from a substance by adding a particular solvent to remove a mixed component of a solid or a liquid. In this study, extraction of chlorophyllid from mango leaves to determine the stability of chlorophyll reduction in the extract and raffinate. Extraction of chlorophyllid was done by stirring extractor that is by cutting mango leaves 1 cm then in extraction with Aquadest solvent 25%: 75% Isopropyl Alcohol. Selection of solvents is a decisive factor in the extraction process. Things to note in the selection of solvents are the selectivity, the properties of the solvent, the ability to extract, not toxic, easily evaporated and the price is relatively cheap. Characteristics of chlorophyll extract using spectrophotometer to determine the absorbance of optimum chlorophyll in an extract and raffinate variables and pH test to find out good pH stability to reduce mango leaf chlorophyll. The optimum chlorophyll absorbance results at 60OC and 70 minutes on the extract and the pH stability result to reduce chlorophyll was found at pH 9 acid and pH 2 base.


2001 ◽  
Vol 13 (11) ◽  
pp. 3120-3125 ◽  
Author(s):  
J. Fernandez ◽  
P. Kurowski ◽  
L. Limat ◽  
P. Petitjeans

2004 ◽  
Vol 58 (3) ◽  
pp. 264-271 ◽  
Author(s):  
E. T. S. Skibsted ◽  
H. F. M. Boelens ◽  
J. A. Westerhuis ◽  
D. T. Witte ◽  
A. K. Smilde

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