708: An injectable silk-based biomaterial as an alternative to cervical cerclage: a pilot study in a pregnant rabbit model

Objectives: In normal mature limb skeletal muscle, satellite cells are quiescent and myonuclei do not divide after formation of their associated myofibers in the absence of injury. The possibility of myonuclear addition in uninjured laryngeal myofibers of adult rabbits was investigated in an immunohistochemical pilot study. Methods: Bromodeoxyuridine (brdU), a marker for cell division, was administered by intraperitoneal injection over a 12-hour period in rabbits. The number of brdU-positive myonuclei per myofiber was determined on cross sections through the thyroarytenoid (TA) and posterior cricoarytenoid (PCA) muscles. Results: In the TA muscle, 0.13% ± 0.03% (mean ± SEM) of the myofibers counted had a brdU-positive nucleus. In the PCA muscle, 0.13% ± 0.01% of the myofibers counted had a brdU-positive nucleus. Approximately 0.2% and 0.3% of the myofibers of the TA and PCA muscles, respectively, had brdU-positive satellite cells associated with them. Tibialis anterior and pectoralis major muscle controls were negative for brdU-positive myonuclei. Conclusions: These data support the possibility of continuous remodeling in uninjured adult laryngeal myofibers and accentuate the distinct nature of laryngeal muscle relative to limb skeletal muscle in the rabbit model.

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Fibrin glue for the suture-less correction of penile chordee: a pilot study in a rabbit model

BJU International ◽

10.1111/j.1464-410x.2004.04938.x ◽

2004 ◽

Vol 94 (3) ◽

pp. 433-436 ◽

Cited By ~ 3

Author(s):

Ashraf T. Hafez ◽

Ahmed El-Assmy ◽

Mohamed A. El-Hamid

Keyword(s):

Pilot Study ◽

Fibrin Glue ◽

Rabbit Model

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Resuscitative efficacy of hemoglobin vesicles for severe postpartum hemorrhage in pregnant rabbits

Scientific Reports ◽

10.1038/s41598-021-01835-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hiroki Ishibashi ◽

Kohsuke Hagisawa ◽

Manabu Kinoshita ◽

Yukako Yuki ◽

Morikazu Miyamoto ◽

...

Keyword(s):

Mean Arterial Pressure ◽

Human Serum Albumin ◽

Cesarean Section ◽

Blood Cell ◽

Postpartum Hemorrhage ◽

Uterine Artery ◽

Rabbit Model ◽

Pregnant Rabbit ◽

The Right ◽

Uncontrolled Hemorrhage

AbstractWe aimed to investigate the resuscitative efficacy of hemoglobin vesicles (HbVs) as a red blood cell (RBC) substitute for the initial treatment of severe postpartum hemorrhage (PPH). Twenty-five pregnant rabbits underwent cesarean section; uncontrolled hemorrhage was induced by transecting the right uterine artery to establish a severe PPH model. During the first 30 min, all rabbits were administered 6% hydroxyethyl starch (HES) of an equivalent volume to the hemorrhage every 5 min. Thereafter, they received any of the following three isovolemic fluids for resuscitation every 5 min: RBCs with platelet-poor plasma (RBC/PPP) (n = 8), 6% HES (n = 7), or HbVs with 25% human serum albumin (n = 10). After surgical hemostasis at 60 min, survival was monitored until 12 h. No rabbits receiving only HES infusion survived beyond 6 h, whereas all rabbits receiving RBC/PPP transfusion survived. The rabbits receiving HbV infusion showed significantly higher mean arterial pressure and hemoglobin levels than the HES-receiving rabbits, and 8 of 10 rabbits survived for 6 h. The HbV group showed significantly higher survival than the HES group but worse survival than the RBC/PPP group. In conclusion, HbV infusion for severe PPH effectively prevents lethal hemorrhagic shock in a pregnant rabbit model.

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