Metagenomic next generation sequencing analysis of vaginal microbiome composition in patients with bacterial vaginosis treated with lactin-v (Lactobacillus crispatus CTV-05) versus placebo

2020 ◽  
Vol 223 (6) ◽  
pp. 967-968
Author(s):  
S.L. Hao ◽  
G.R. Castaneda ◽  
C.R. Cohen ◽  
A. Hemmerling ◽  
E.D. Crawford
2020 ◽  
Author(s):  
Yuling An ◽  
Mingming Fan ◽  
Ziyu Li ◽  
You Peng ◽  
Xiaomeng Yi ◽  
...  

Abstract We shared our successful treatment experience of a severe tetanus patient in China. A 50 year old male patient was admitted to our hospital 10 days after the right arm injury due to pain and masticatory weakness. The pathogen of wound secretion was confirmed to be clostridium tetanus by next-generation sequencing (NGS).The patient's condition rapidly progressed to a severe state with autonomic instability. After debridement and comprehensive treatment in ICU, including deep analgesia and sedation with dexmedetomidine, ventilator support and anti-infection treatment, the patient finally recovered and discharged. This case suggested that early diagnosis and reasonable intervention of severe tetanus could reduce mortality.


F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 50 ◽  
Author(s):  
Michael T. Wolfinger ◽  
Jörg Fallmann ◽  
Florian Eggenhofer ◽  
Fabian Amman

Recent achievements in next-generation sequencing (NGS) technologies lead to a high demand for reuseable software components to easily compile customized analysis workflows for big genomics data. We present ViennaNGS, an integrated collection of Perl modules focused on building efficient pipelines for NGS data processing. It comes with functionality for extracting and converting features from common NGS file formats, computation and evaluation of read mapping statistics, as well as normalization of RNA abundance. Moreover, ViennaNGS provides software components for identification and characterization of splice junctions from RNA-seq data, parsing and condensing sequence motif data, automated construction of Assembly and Track Hubs for the UCSC genome browser, as well as wrapper routines for a set of commonly used NGS command line tools.


2019 ◽  
Vol 305 ◽  
pp. S7
Author(s):  
S. Paolacci ◽  
P.E. Maltese ◽  
E. Manara ◽  
G. Iarossi ◽  
L. Ziccardi ◽  
...  

2019 ◽  
Vol 81 (11) ◽  
pp. 1547-1551 ◽  
Author(s):  
Ayumi MIURA ◽  
Tomomi KURUMISAWA ◽  
Rui KANO ◽  
Takaaki ITO ◽  
Kazuyuki SUZUKI ◽  
...  

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