scholarly journals Lipid analysis of seminal plasma from Nellore bulls (Bos taurus indicus) with high and low resistance to cryopreservation

2018 ◽  
Vol 194 ◽  
pp. e11-e12
Author(s):  
Mariana Furtado Zorzetto ◽  
Erika Aline Ribeiro Dias ◽  
Fabio Morato Monteiro ◽  
Camila de Paula Freitas Dell’Aqua ◽  
Claudia Cristina Paro de Paz ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Bos Taurus ◽  
Lipid Analysis ◽  
Low Resistance ◽  
Bos Taurus Indicus

scholarly journals Differential abundances of four forms of Binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability

2016 ◽  
Vol 86 (3) ◽  
pp. 766-777.e2 ◽  
Author(s):  
Marcos Jorge Magalhães ◽  
Leonardo Franco Martins ◽  
Renato Lima Senra ◽  
Thaís Ferreira dos Santos ◽  
Denise Silva Okano ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Bos Taurus ◽  
Bos Taurus Indicus

Proteome Analysis of Nelore Bull (Bos taurus indicus) Seminal Plasma

2005 ◽  
Vol 12 (8) ◽  
pp. 813-817 ◽  
Author(s):  
T. Assumpcao ◽  
W. Fontes ◽  
M. Sousa ◽  
C. Ricart
Keyword(s):  
Seminal Plasma ◽  
Bos Taurus ◽  
Proteome Analysis ◽  
Bos Taurus Indicus

scholarly journals Heparin-binding proteins of seminal plasma in Nellore bulls

2008 ◽  
Vol 39 (1) ◽  
pp. 275-278
Author(s):  
Carlos Eurico Fernandes ◽  
Fabiana Ferreira de Souza ◽  
Jayme Augusto Souza-Neto ◽  
Paulo Eduardo Martins Ribola
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Bos Taurus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Heparin Binding ◽  
One Dimensional ◽  
Molecular Weights ◽  
Bos Taurus Indicus ◽  
Heparin Affinity

The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.

scholarly journals Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Emanuel M. Doroteu ◽  
Joao H. M. Viana ◽  
Jair A. Ferreira Junior ◽  
Juliana T. A. Macedo ◽  
Rodrigo A. Oliveira ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Active Immunization ◽  
Control Group ◽  
Histopathological Analysis ◽  
Sample Collection ◽  
Functional Characteristics ◽  
Scrotal Circumference ◽  
Bos Taurus Indicus ◽  
Boost Dose ◽  
And Control

AbstractThe aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (Bos taurus indicus) bulls were randomly allocated into three experimental groups: G1 (n=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (n=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG, n=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (P=0.0476), width (P=0.0021), and in scrotal circumference (P=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (P<0.01), but in G2, it was also lower than in G1 at day 90 (P=0.0006). All sperm parameters were affected by active immunization against GnRH (P<0.05), and in G2, averages were lesser (P<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.

scholarly journals Fetal calf serum enhances in vitro production of Bos taurus indicus embryos

2011 ◽  
Vol 75 (3) ◽  
pp. 429-433 ◽  
Author(s):  
F.G. Leivas ◽  
D.S. Brum ◽  
S.S. Fialho ◽  
W.P. Saliba ◽  
M.T.T. Alvim ◽  
...  
Keyword(s):  
Fetal Calf Serum ◽  
Bos Taurus ◽  
Calf Serum ◽  
In Vitro Production ◽  
Bos Taurus Indicus ◽  
Vitro Production

Identification of novel candidate genes for age at first calving in Nellore cows using a SNP chip specifically developed for Bos taurus indicus cattle

2021 ◽  
Author(s):  
Miguel Angel Carabantes Dubom ◽  
Victor Breno Pedrosa ◽  
Fabieli Loise Braga Feitosa ◽  
Raphael Bermal Costa ◽  
Gregório Miguel Ferreira de Camargo ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Bos Taurus ◽  
Age At First Calving ◽  
Snp Chip ◽  
Bos Taurus Indicus

scholarly journals Mitochondrial DNA of Nellore and European x Nellore crossing cattle of high performance

2007 ◽  
Vol 42 (8) ◽  
pp. 1203-1205 ◽  
Author(s):  
Rogério Abdallah Curi ◽  
Lígia Souza Lima Silveira da Mota ◽  
Antônio Carlos Silveira
Keyword(s):  
Mitochondrial Dna ◽  
High Frequency ◽  
High Performance ◽  
Bos Taurus ◽  
Meat Production ◽  
Bos Taurus Indicus ◽  
Nd5 Gene

The objective of this work was to evaluate, through a polymorphism in the ND5 gene of the bovine mitochondrial DNA, the frequency of Bos taurus indicus mtDNA individuals in a sample of Nellore purebred origin animals (n = 69) and crossbred animals originated from crosses of European sires and Nellore purebred origin females (n = 275). Only 2.26% (8/354) of the animals presented Bos taurus indicus mtDNA. The high frequency of Bos taurus taurus mtDNA in these animals can be a consequence of selection, once the animals studied are originated from selected lineages of high performance for meat production.

scholarly journals PROPORÇÃO DO SEXO NA PRODUÇÃO IN VITRO DE EMBRIÕES BOVINOS

2015 ◽  
Vol 20 (2) ◽  
Author(s):  
Marcus Vinícius Galvão Loiola ◽  
Alexandra Soares Rodrigues ◽  
Marcos Chalhoub ◽  
Priscila Assis Ferraz ◽  
Rodrigo Freitas Bittencourt ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Taurus Indicus

Objetivou-se avaliar a proporção do sexo utilizando espermatozoides provenientes de sêmen convencional ou sexado para fêmea em um programa de produção in vitro de embriões (PIVE) bovinos. Foram utilizados oócitos de 87 doadoras Bos taurus indicus da raça Nelore, obtidos por sistema de aspiração folicular transvaginal guiado por ultrassonografia. Os oócitos selecionados foram maturados in vitro, fertilizados com espermatozoides oriundos de sêmen convencional (grupo 1 – CONV) ou sêmen sexado para fêmea (grupo 2 – SEX) e os embriões produzidos foram então cultivados in vitro por sete dias. Após o cultivo, os mesmos foram envasados e inovulados em receptoras mestiças Bos taurus taurus x Bos taurus indicus. Foram transferidos 1.319 embriões, sendo 1.081 produzidos com sêmen convencional (CONV) e 238 com sêmen sexado de fêmea (SEX). O diagnóstico precoce de gestação e a sexagem fetal foram realizados por ultrassonografia transretal. A taxa de gestação total foi 37,38% (493/1.319), sendo 37,74% (408/1.081) no grupo CONV e 35,71% (85/238) no grupo SEX. A proporção do sexo no grupo CONV foi desviada (P=0,002) a favor dos machos (57,84% de machos e 42,16% de fêmeas) e no grupo SEX ocorreu um desvio na proporção (P=0,001) a favor das fêmeas (68,24% de fêmeas e 31,76% de machos). Concluiu-se que a utilização do sêmen convencional em PIVE bovinos elevou a proporção de produtos machos e apesar da utilização de sêmen sexado de fêmea ser uma alternativa para pré-selecionar o sexo dos produtos, ainda houve uma considerável taxa de machos quando se utilizou este tipo de sêmen.

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