Effect of FSH treatment on cumulus oocyte complex recovery by ovum pick up and in vitro embryo production in beef donor cows

2020 ◽  
Vol 214 ◽  
pp. 106274
Author(s):  
Felipe L. Ongaratto ◽  
Andrés V. Cedeño ◽  
Paula Rodriguez-Villamil ◽  
Andrés Tríbulo ◽  
Gabriel A. Bó
Keyword(s):  
Embryo Production ◽  
Cumulus Oocyte Complex ◽  
In Vitro Embryo Production ◽  
Donor Cows

scholarly journals Effect of complexed trace minerals on cumulus-oocyte complex recovery and in vitro embryo production in beef cattle1,2

2019 ◽  
Vol 97 (4) ◽  
pp. 1478-1490 ◽  
Author(s):  
Felipe G Dantas ◽  
Sydney T Reese ◽  
Ramiro V O Filho ◽  
Rafael S Carvalho ◽  
G A Franco ◽  
...  
Keyword(s):  
Trace Minerals ◽  
Embryo Production ◽  
Cumulus Oocyte Complex ◽  
In Vitro Embryo Production

scholarly journals Identification of potential biomarkers in donor cows for in vitro embryo production by granulosa cell transcriptomics

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175464 ◽  
Author(s):  
Gianluca Mazzoni ◽  
Suraya M. Salleh ◽  
Kristine Freude ◽  
Hanne S. Pedersen ◽  
Lotte Stroebech ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Potential Biomarkers ◽  
Donor Cows

9 Ovum pick-up/in vitro embryo production (OPU-IVP), an alternative means for infertile or bad donor cows to produce embryos

2022 ◽  
Vol 34 (2) ◽  
pp. 238
Author(s):  
G. Gamarra ◽  
S. Lacaze ◽  
E. Gouache ◽  
N. Leroy ◽  
N. Picard-Hagen
Keyword(s):  
Embryo Production ◽  
Alternative Means ◽  
In Vitro Embryo Production ◽  
Donor Cows

173 IN VITRO EMBRYO PRODUCTION FROM OOCYTES FERTILISED WITH UNSORTED OR X-SORTED SPERM AND ISSUED FROM SUBFERTILE HIGH GENETIC MERIT COWS SUBMITTED OR NOT TO A 48-h COASTING PERIOD FOLLOWING FSH STIMULATION

2016 ◽  
Vol 28 (2) ◽  
pp. 217
Author(s):  
G. Gamarra ◽  
S. Lacaze ◽  
C. Ponsart ◽  
M. Mouneyres ◽  
B. Le Guienne
Keyword(s):  
Embryonic Development ◽  
In Vitro Production ◽  
Genetic Progress ◽  
Embryo Production ◽  
Chi Square ◽  
Genetic Merit ◽  
In Vitro Embryo Production ◽  
Donor Cows

High genetic merit cows may be sidelined from breeding schemes because of reproductive disorders. In vitro production of embryos (IVP) issued from ovum pickup (OPU) can be an alternative to bypass infertility problems as experienced in humans and thus accelerate genetic progress (Duszewska et al. 2012 J. Anim. Feed Sci. 21, 217–233). The aim of this work was to evaluate if IVP from high genetic merit subfertile cows could benefit to the breeding scheme under commercial conditions, at the Biotechnology MIDATEST Station located in Denguin, Southwest, France. Holstein cows (n = 16) from 3.5 to 13 years old with different reproductive pathologic problems (repeated breeding, failure in in vivo embryo production, embryo mortality, permanent cysts, oviduct infection) were used in an OPU-IVP program. Donor cows were stimulated with decreasing doses pFSH twice daily during 3 days (Stimufol®, total dose: 350 µg of pFSH). The OPU was performed 48 h after the last FSH injection in the “coasting” group v. 12 h after the last FSH injection for “no-coasting” group and in vitro matured using a standard IVM protocol. Oocytes were fertilised with frozen-thawed unsorted or X-sorted sperm in TALP medium from different bulls (n = 42) without any previous IVP testing. Presumptive zygotes were cultured in SOF medium (Minitub®) plus 1% cow serum up to Day 7 at 38.5°C in 5% CO2, 5% O2, and 90% N2 atmosphere with maximum humidity. The OPU-IVP was repeated 1 to 6 times (3.75 ± 2.6) for each donor cow. Grade 1 blastocysts and expanded blastocysts (according to IETS classification) were recorded on Days 6.5 and 7. Embryo production was analysed with ANOVA and blastocyst yield was analysed by chi-square. The results in both coasting or no-coasting groups and the effect of fertilization using unsorted or X-sorted sperm are presented in Table 1. The embryonic development rate was significantly higher when using unsorted semen to fertilize the oocytes compared to X-sorted sperm (P < 0.05). On the other hand the coasting period had no significant effect neither on the number of collected oocytes nor on the embryonic development rates. In conclusion, our work confirmed the efficacy of OPU-IVP techniques to produce grade 1 embryos using X-sorted or unsorted sperm in subfertile high genetic merit cows. Table 1.Oocyte collection and in vitro embryo production, in donor cows submitted or not to a 48-h coasting period and effect of fertilization with unsorted v. X-sorted sperm

140 A comparison of in vitro embryo production between heifers and lactating Holstein donors without superstimulation

2019 ◽  
Vol 31 (1) ◽  
pp. 195
Author(s):  
E. P. Silva ◽  
M. K. Sermersheim ◽  
P. V. Marchioretto ◽  
R. Della Mea ◽  
L. M. Naves ◽  
...  
Keyword(s):  
Donor Age ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Exact Test ◽  
Lactating Cows ◽  
Cumulus Oocyte Complex ◽  
Sexed Semen ◽  
In Vitro Embryo Production ◽  
Blastocyst Rate

Bovine oocyte donor age seems to have an important role on in vitro embryo production. Therefore, the aim of this study was to compare cumulus-oocyte complex (COC) recovery, cleavage, and blastocyst rates between heifers and lactating Holstein donors. A total of 89 animals (heifers: n=60, 11 to 17 months of age; lactating cows: n=29, 60 to 180 days in milk) were used in this experiment. Ovum pickup (Day −1) was performed on a random day of the oestrous cycle without superstimulation by a single technician. Conventional or sexed semen were used for IVF (Day 0). Cleavage and blastocyst rates were evaluated on Days 3 and 7, respectively. Cleavage was determined when structures had 4 or more cells and blastocyst rate included grades I and II blastocysts. Continuous data were analysed by t-test, and binomial data were analysed by Fischer’s exact test. A total of 1,289 COC were recovered from lactating cows (n=509) and heifers (n=777). Total and viable COC per donor were higher (P&lt;0.05) in lactating cows (total=17.55±13.08; viable=11.24±8.9) than heifers (total=12.95±5.39; viable=7.88±4.08). The percentage of viable COC was similar (P&gt;0.05) in lactating cows (64.04%; 326/509) and heifers (60.87%; 473/777). Cleavage rate was higher (P&lt;0.0001) in lactating cows (58.74%; 299/509) than in heifers (45.05%; 350/427). Cleavage rate was similar (P&gt;0.05) with conventional and sexed semen in heifers (conventional=37.68%; 52/138; sexed=46.63%; 298/639) and cows (conventional=55.05%; 49/89; sexed=59.52%; 250/470). Blastocyst rate was higher (P&lt;0.0001) in lactating cows (19.84%; 101/509) than in heifers (8.49%; 66/777). Conventional semen had a higher (P&lt;0.0001) blastocyst rate (18.11%; 24/138) than sexed semen (6.57%; 42/639) in heifers. However, there was no difference (P&gt;0.05) in blastocyst rate between conventional (24.71%; 22/89) and sexed semen (18.8%; 79/420) in lactating cows. Blastocyst/cleaved structures ratio was higher (P&lt;0.05) in heifers using conventional (46.15%; 24/52) compared with sexed (14.06%; 42/298) semen. However, in lactating cows there was a tendency (P&lt;0.1) for higher blastocyst/cleaved structures ratio using conventional (44.89%; 22/49) compared with sexed semen (31.6%; 79/250). In conclusion, a higher number of total and viable COC were aspirated from Holstein lactating cows than heifers. Furthermore, cleavage and blastocyst rates were higher in lactating cows than in heifers. Conventional semen gave more embryos than did sexed semen in heifers but not in cows. Overall, oocytes from Holstein lactating donors are more suitable for in vitro embryo production than are those from heifer donors.

scholarly journals Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 216
Author(s):  
Hernan Baldassarre
Keyword(s):  
Pregnancy Rate ◽  
Commercial Application ◽  
Embryo Production ◽  
Breeding Programs ◽  
Sheep And Goats ◽  
Embryo Recovery ◽  
Marker Selection ◽  
Holstein Calves ◽  
In Vitro Embryo Production

The potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as the procedure is minimally invasive and can be repeated more times and more frequently in the same animals compared with conventional surgical embryo recovery. On average, ~10 and ~14 viable oocytes are recovered by LOPU from sheep and goats, respectively, which results in 3–5 transferable embryos and >50% pregnancy rate after transfer. LOPU-IVEP has also been applied to prepubertal ruminants of 2–6 months of age, including bovine and buffalo calves. In dairy cattle, the technology has gained momentum in the past few years stemming from the development of genetic marker selection that has allowed predicting the production phenotype of dairy females from shortly after birth. In Holstein calves, we obtained an average of ~22 viable oocytes and ~20% transferable blastocyst rate, followed by >50% pregnancy rate after transfer, declaring the platform ready for commercial application. The present and future of this technology are discussed with a focus on improvements and research needed.

Selection of stallions for in vitro embryo production by ICSI in a commercial program

2012 ◽  
Vol 32 (7) ◽  
pp. 409 ◽  
Author(s):  
C. Herrera ◽  
P. Dufourq ◽  
M. Freije ◽  
I. Morikawa ◽  
J.E. Centeno ◽  
...  
Keyword(s):  
Embryo Production ◽  
Commercial Program ◽  
In Vitro Embryo Production ◽  
Selection Of

Cysteamine supplementation during in vitro maturation and embryo culture: A useful tool for increasing the efficiency of bovine in vitro embryo production

2002 ◽  
Vol 62 (2) ◽  
pp. 203-209 ◽  
Author(s):  
D.G. de Matos ◽  
C. Herrera ◽  
R. Cortvrindt ◽  
J. Smitz ◽  
A. Van Soom ◽  
...  
Keyword(s):  
Embryo Culture ◽  
In Vitro Maturation ◽  
Embryo Production ◽  
In Vitro Embryo Production

In vitro embryo production after microinjection and ovarian dynamics following transvaginal follicular oocyte aspiration

1995 ◽  
Vol 43 (6) ◽  
pp. 1129-1139 ◽  
Author(s):  
J.R. Gibbons ◽  
R.L. Krisher ◽  
S.K. Carlin ◽  
R.E. Pearson ◽  
F.C. Gwazdauskas
Keyword(s):  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Oocyte Aspiration ◽  
Ovarian Dynamics
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