Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs

2016 ◽  
Vol 131 ◽  
pp. 74-84 ◽  
Author(s):  
Chunxiao Mou ◽  
Liqi Zhu ◽  
Xianping Xing ◽  
Jian Lin ◽  
Qian Yang
Keyword(s):  
Bacillus Subtilis ◽  
Immune Responses ◽  
Spike Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Gastroenteritis Virus ◽  
Transmissible Gastroenteritis ◽  
Recombinant Bacillus Subtilis

scholarly journals Recombinant Canine Coronaviruses Related to Transmissible Gastroenteritis Virus of Swine Are Circulating in Dogs

2008 ◽  
Vol 83 (3) ◽  
pp. 1532-1537 ◽  
Author(s):  
Nicola Decaro ◽  
Viviana Mari ◽  
Marco Campolo ◽  
Alessio Lorusso ◽  
Michele Camero ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
The Other ◽  
Spike Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Type Ii ◽  
Internal Organs ◽  
Systemic Involvement ◽  
Gastroenteritis Virus ◽  
Porcine Transmissible Gastroenteritis Virus ◽  
Transmissible Gastroenteritis

ABSTRACT Four canine coronavirus type II (CCoV-II) strains were identified in the guts and internal organs of pups which had died of acute gastroenteritis. The CCoV-II strains were strictly related to porcine transmissible gastroenteritis virus (TGEV) in the N-terminal domain of the spike protein, whereas in the other parts of the genome, a higher genetic relatedness to recent CCoV-II isolates was observed. Experimental infection of dogs with a TGEV-like isolate induced mild gastroenteritis without any systemic involvement. By virus neutralization tests, antigenic differences between reference and TGEV-like CCoVs were found. Our data support the potential recombinant origin of the TGEV-like CCoVs.

scholarly journals The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 313 ◽  
Author(s):  
Gang Wang ◽  
Rui Liang ◽  
Ziwei Liu ◽  
Zhou Shen ◽  
Jiale Shi ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Reverse Genetics ◽  
Clinical Signs ◽  
Etiologic Agent ◽  
Spike Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Spike Gene ◽  
Terminal Domain ◽  
Gastroenteritis Virus ◽  
Transmissible Gastroenteritis

Transmissible gastroenteritis virus (TGEV) is the etiologic agent of transmissible gastroenteritis in pigs, and the N-terminal domain of TGEV spike protein is generally recognized as both the virulence determinant and enteric tropism determinant. Here, we assembled a full-length infectious cDNA clone of TGEV in a bacterial artificial chromosome. Using a novel approach, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems efficiently and rapidly rescued another recombinant virus with a 224-amino-acid deletion in the N-terminal domain of the TGEV Spike gene (S_NTD224), which is analogous to the N-terminal domain of porcine respiratory coronavirus. S_NTD224 notably affected the TGEV growth kinetics in PK-15 cells but was not essential for recombinant virus survival. In animal experiments with 13 two-day-old piglets, the TGEV recombinant viruses with/without S_NTD224 deletion induced obvious clinical signs and mortality. Together, our results directly demonstrated that S_NTD224 of TGEV mildly influenced TGEV virulence but was not the enteric tropism determinant and provide new insights for the development of a new attenuated vaccine against TGEV. Importantly, the optimized reverse genetics platform used in this study will simplify the construction of mutant infectious clones and help accelerate progress in coronavirus research.

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