A novel three-dimensional cell culture method enhances antiviral drug screening in primary human cells

2018 ◽  
Vol 150 ◽  
pp. 20-29 ◽  
Author(s):  
Robert Koban ◽  
Markus Neumann ◽  
Aila Daugs ◽  
Oliver Bloch ◽  
Andreas Nitsche ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Antiviral Drug ◽  
Three Dimensional ◽  
Culture Method ◽  
Human Cells ◽  
Dimensional Cell ◽  
Primary Human Cells

scholarly journals Time‐lapse imaging assay using the BioStation CT : A sensitive drug‐screening method for three‐dimensional cell culture

2015 ◽  
Vol 106 (6) ◽  
pp. 757-765 ◽  
Author(s):  
Ruriko Sakamoto ◽  
M. Mamunur Rahman ◽  
Manami Shimomura ◽  
Manabu Itoh ◽  
Tetsuya Nakatsura
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Screening Method ◽  
Three Dimensional ◽  
Time Lapse ◽  
Time Lapse Imaging ◽  
Dimensional Cell

scholarly journals Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiaowen Wu ◽  
Junxiang Su ◽  
Jizhen Wei ◽  
Nan Jiang ◽  
Xuejun Ge
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Culture Method ◽  
Future Research ◽  
Two Dimensional ◽  
Culture Methods ◽  
Three Dimensional Culture ◽  
Dimensional Cell

Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly.

A Three-Dimensional Cell Culture Method for Studying Peritoneal Adhesions

1997 ◽  
pp. 49-63 ◽  
Author(s):  
F. Bittinger ◽  
C. L. Klein ◽  
C. Skarke ◽  
C. Brochhausen ◽  
M. Otto ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Culture Method ◽  
Peritoneal Adhesions ◽  
Dimensional Cell

scholarly journals Three-dimensional cell culture models for anticancer drug screening: Worth the effort?

2017 ◽  
Vol 233 (4) ◽  
pp. 2993-3003 ◽  
Author(s):  
Eddy-Tim Verjans ◽  
Jordi Doijen ◽  
Walter Luyten ◽  
Bart Landuyt ◽  
Liliane Schoofs
Keyword(s):  
Cell Culture ◽  
Anticancer Drug ◽  
Drug Screening ◽  
Three Dimensional ◽  
Culture Models ◽  
Cell Culture Models ◽  
Dimensional Cell

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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