A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro

2013 ◽  
pp. 183-190 ◽  
Author(s):  
Yoji Okugawa ◽  
Yohei Hirai
Keyword(s):  
Cell Culture ◽  
Cell Differentiation ◽  
Epidermal Cell ◽  
Three Dimensional ◽  
Culture Method ◽  
Dimensional Cell ◽  
Epidermal Cell Differentiation

scholarly journals Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiaowen Wu ◽  
Junxiang Su ◽  
Jizhen Wei ◽  
Nan Jiang ◽  
Xuejun Ge
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Culture Method ◽  
Future Research ◽  
Two Dimensional ◽  
Culture Methods ◽  
Three Dimensional Culture ◽  
Dimensional Cell

Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly.

A novel three-dimensional cell culture method enhances antiviral drug screening in primary human cells

2018 ◽  
Vol 150 ◽  
pp. 20-29 ◽  
Author(s):  
Robert Koban ◽  
Markus Neumann ◽  
Aila Daugs ◽  
Oliver Bloch ◽  
Andreas Nitsche ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Antiviral Drug ◽  
Three Dimensional ◽  
Culture Method ◽  
Human Cells ◽  
Dimensional Cell ◽  
Primary Human Cells

scholarly journals Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 481
Author(s):  
Tarek Saydé ◽  
Omar El Hamoui ◽  
Bruno Alies ◽  
Karen Gaudin ◽  
Gaëtane Lespes ◽  
...  
Keyword(s):  
Cell Culture ◽  
Complex Structure ◽  
Tumor Development ◽  
Three Dimensional ◽  
3D Culture ◽  
Supramolecular Assembly ◽  
Hydrogel Scaffold ◽  
Scale Characterization ◽  
Dimensional Cell

Three-dimensional cell culture has revolutionized cellular biology research and opened the door to novel discoveries in terms of cellular behavior and response to microenvironment stimuli. Different types of 3D culture exist today, including hydrogel scaffold-based models, which possess a complex structure mimicking the extracellular matrix. These hydrogels can be made of polymers (natural or synthetic) or low-molecular weight gelators that, via the supramolecular assembly of molecules, allow the production of a reproducible hydrogel with tunable mechanical properties. When cancer cells are grown in this type of hydrogel, they develop into multicellular tumor spheroids (MCTS). Three-dimensional (3D) cancer culture combined with a complex microenvironment that consists of a platform to study tumor development and also to assess the toxicity of physico-chemical entities such as ions, molecules or particles. With the emergence of nanoparticles of different origins and natures, implementing a reproducible in vitro model that consists of a bio-indicator for nano-toxicity assays is inevitable. However, the maneuver process of such a bio-indicator requires the implementation of a repeatable system that undergoes an exhaustive follow-up. Hence, the biggest challenge in this matter is the reproducibility of the MCTS and the associated full-scale characterization of this system’s components.

A novel in vitro permeability assay using three-dimensional cell culture system

2015 ◽  
Vol 205 ◽  
pp. 93-100 ◽  
Author(s):  
Jong Bong Lee ◽  
Sung Hwa Son ◽  
Min Chul Park ◽  
Tae Hwan Kim ◽  
Min Gi Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Permeability Assay ◽  
Dimensional Cell

scholarly journals Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model

2007 ◽  
Vol 22 (11) ◽  
pp. 3031-3037 ◽  
Author(s):  
P.G.L. Lalitkumar ◽  
S. Lalitkumar ◽  
C.X. Meng ◽  
A. Stavreus-Evers ◽  
F. Hambiliki ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Human Blastocyst ◽  
Culture Model ◽  
Dimensional Cell

Advances in reconstructing intestinal functionalities in vitro: From two/three dimensional-cell culture platforms to human intestine-on-a-chip

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122097
Author(s):  
Li Wang ◽  
Jian Wu ◽  
Jun Chen ◽  
Wenkun Dou ◽  
Qili Zhao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Human Intestine ◽  
Dimensional Cell
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