Background. Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance. In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV).Methods. The Hi3-1 assay was tested using four panels (Panel 1,n=4,581patient samples; Panel 2,n=15seroconversion samples; Panel 3,n=4performance samples; and Panel 4,n=251purchased positive control samples), and the results were collected by the Department of Laboratory Medicine, Korea University Medical College, Republic of Korea. The present study compares the sensitivity of the multiplex detection platform for both HIV and HCV using a sol-gel based microarray, which was based on a reference test (Architect HIV Ag/Ab Combo and Architect anti-HCV assays), in Korean patients.Results. The sensitivity of the multiplex detection platform for both HIV and HCV was 100%, and the specificity was 99.96% for HIV and 99.76% for HCV, which is equivalent to that of the reference test.Conclusion. We have successfully applied a novel screening technology to multiplex HIV and HCV diagnoses in a blood bank screening test.
P-70 SEROPREVALENCE OF HEPATITIS C VIRUS IN DONORS OF THE BLOOD BANK OF THE GENERAL HOSPITAL OF MEXICO "DR. EDUARDO LICEAGA " A FOUR-YEAR EVALUATION