scholarly journals Anti-diabetic effects and mechanisms of action of a Chinese herbal medicine preparation JQ-R in vitro and in diabetic KK Ay mice

2017 ◽  
Vol 7 (4) ◽  
pp. 461-469 ◽  
Author(s):  
Quan Liu ◽  
Shuainan Liu ◽  
Lihui Gao ◽  
Sujuan Sun ◽  
Yi Huan ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Mechanisms Of Action ◽  
Chinese Herbal ◽  
Ay Mice

High-Throughput Electrophysiology Screen Revealed Cardiotoxicity of Strychnine by Selectively Targeting hERG Channel

2018 ◽  
Vol 46 (08) ◽  
pp. 1825-1840 ◽  
Author(s):  
Taiyi Wang ◽  
Xiaonan Chen ◽  
Jiahui Yu ◽  
Qunqun Du ◽  
Jie Zhu ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Patch Clamp ◽  
High Throughput ◽  
Chinese Herbal Medicine ◽  
Large Scale ◽  
Cardiac Toxicity ◽  
Herg Channel ◽  
Chinese Herbal ◽  
Cell Vitality

Although the efficacy and the health care advantages of Chinese herbal medicine (CHM) have become increasingly recognized worldwide, the potential side effects and toxicity still restrict its broader application. This study established and applied an integrated platform anchored on automatic patch clamp system to screen and evaluate a collection of CHM extracts, compositions and monomeric compounds for in vitro cardiac toxicity. Of 1036 CHM samples screened, 2.79% significantly inhibited hERG channel activity. Among them, Strychnine was identified for the first time as a potent hERG inhibitor with an IC[Formula: see text] of [Formula: see text]M in comparison to that of Dofetilide at [Formula: see text]M and Quinidine at [Formula: see text]M. Langendorff-perfusion experiments confirmed that strychnine increased QT interphase from [Formula: see text][Formula: see text]ms to [Formula: see text][Formula: see text]ms and decreased heart rates from [Formula: see text][Formula: see text]bmp to [Formula: see text][Formula: see text]bmp in isolated rat hearts. The cardiac toxicity effect of strychnine appears to be specific to hERG channel since an in vitro multiplex imaging analysis showed that it did not affect cellular phenotypes such as cell vitality, nucleus area, mitochondria mass and function, nor intracellular calcium in rat primary myocytes. This integrated high-throughput hERG patch clamp and high-content multi-parameter imaging cardiac toxicity screen approach should be useful for large-scale preclinical evaluation of complex Chinese herbal medicine.

Wen-Jing-Tang, a Traditional Chinese Herbal Medicine Increases Luteinizing Hormone Release In Vitro

1986 ◽  
Vol 14 (03n04) ◽  
pp. 157-160 ◽  
Author(s):  
Akira Miyake ◽  
Jin-Woo Lee ◽  
Keiichi Tasaka ◽  
Shirou Ohtsuka ◽  
Toshihiro Aono
Keyword(s):  
Herbal Medicine ◽  
Luteinizing Hormone ◽  
Chinese Herbal Medicine ◽  
Female Rats ◽  
Normal Female ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Lh Release ◽  
Lh Rh

For examination of the effect on luteinizing hormone (LH) release of Wen-Jing-Tang, a traditional Chinese herbal medicine, the pituitary from normal female rats in diestrus was perifused alone or in sequence with the mediobasal hypothalamus (MBH) in a sequential double-chamber perifusion system. Wen-Jing-Tang at 5 or 500 μg/ml induced significant LH release (60-95 % increase) from the pituitary in series with the MBH, but had no effect on LH release from the pituitary perifused alone. These data suggest that Wen-Jing-Tang induces LH release from the pituitary through hypothalamic LH-RH.

Attenuation of In Vitro and In Vivo Melanin Synthesis using a Chinese Herbal Medicine through the Inhibition of Tyrosinase Activity

Phytomedicine ◽  
2021 ◽  
pp. 153876
Author(s):  
Shu-Chun Liu ◽  
Meei-Ling Sheu ◽  
Yi-Ching Tsai ◽  
Yu-Chin Lin ◽  
Ching-Wen Chang ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Tyrosinase Activity ◽  
Melanin Synthesis ◽  
Chinese Herbal

Effect of a Traditional Chinese Herbal Medicine (San Zhuang Wan) on the Hypothalamus-Pituitary-Testis Axis of the Immature Rat

1989 ◽  
Vol 17 (03n04) ◽  
pp. 171-175
Author(s):  
Ting-Xin Zhang ◽  
David K. Pomerantz
Keyword(s):  
Herbal Medicine ◽  
Cyclic Amp ◽  
Chinese Herbal Medicine ◽  
Testicular Tissue ◽  
Male Rat ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Male Impotence ◽  
Testicular Weight

San Zhuang Wan (SZW) is a traditional Chinese herbal medicine used to treat male impotence. The mechanism of its therapeutic effect is not known nor have any of its potential physiologic actions been investigated. We examined the effect of SZW adminstered by gavage, on the reproductive system of the prepubertal male rat. After 10 days treatment of 21-day-old rats the concentration of testosterone in serum and testicular weight and decrease whereas the serum concentration of luteinizing hormone (LH) had significantly increased. When administered to gonadectomized rats SZW partially restored the weight of the accessory sex organs and concentration of LH toward normal, but serum androgen concentration was not changed. In vitro assessment of fragments of testicular tissue or purified Leydig cells for responsiveness to maximal doses of LH or cyclic AMP showed that SWZ significantly reduced testosterone secretion in response to either stimulant. The data demonstrate that SWZ has potent effects on the reproductive axis of the pubertal male rat. At least one locus for such action is on the Leydig cell at a point distal to the formation of cyclic AMP. Further study is necessary to determine whether these effects could be involved with or independent of the mechanism of the therapeutic action of SZW.

scholarly journals TheIn VitroandIn VivoWound Healing Properties of the Chinese Herbal Medicine “Jinchuang Ointment”

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Tsung-Jung Ho ◽  
Shinn-Jong Jiang ◽  
Guang-Huey Lin ◽  
Tzong Shiun Li ◽  
Lih-Ming Yiin ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Scientific Basis ◽  
Tube Formation ◽  
University Hospital ◽  
Chemical Compositions ◽  
Dragon’S Blood ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal

“Jinchuang ointment” is a traditional Chinese herbal medicine complex for treatment of incised wounds. For more than ten years, it has been used at China Medical University Hospital (Taichung, Taiwan) for the treatment of diabetic foot infections and decubitus ulcers. Three different cases are presented in this study. “Jinchuang” ointment is a mixture of natural product complexes from nine different components, making it difficult to analyze its exact chemical compositions. To further characterize the herbal ingredients used in this study, the contents of reference standards present in a subset of the ointment ingredients (dragon’s blood, catechu, frankincense, and myrrh) were determined by HPLC. Twoin vitrocell based assay platforms, wound healing and tube formation, were used to examine the biological activity of this medicine. Our results show that this herbal medicine possesses strong activities including stimulation of angiogenesis, cell proliferation, and cell migration, which provide the scientific basis for its clinically observed curative effects on nonhealing diabetic wounds.

scholarly journals Chinese Herbal Medicine for the Treatment of Depression: Effects on the Neuroendocrine-Immune Network

2021 ◽  
Vol 14 (1) ◽  
pp. 65
Author(s):  
Chan Li ◽  
Bishan Huang ◽  
Yuan-Wei Zhang
Keyword(s):  
Herbal Medicine ◽  
Effective Treatment ◽  
Chinese Herbal Medicine ◽  
Biological Systems ◽  
Mechanisms Of Action ◽  
Preclinical Studies ◽  
Immune Network ◽  
Neuroinflammatory Response ◽  
Chinese Herbal ◽  
Treatment Of Depression

The neuroimmune and neuroendocrine systems are two critical biological systems in the pathogenesis of depression. Clinical and preclinical studies have demonstrated that the activation of the neuroinflammatory response of the immune system and hyperactivity of the hypothalamus–pituitary–adrenal (HPA) axis of the neuroendocrine system commonly coexist in patients with depression and that these two systems bidirectionally regulate one another through neural, immunological, and humoral intersystem interactions. The neuroendocrine-immune network poses difficulties associated with the development of antidepressant agents directed toward these biological systems for the effective treatment of depression. On the other hand, multidrug and multitarget Chinese Herbal Medicine (CHM) has great potential to assist in the development of novel medications for the systematic pharmacotherapy of depression. In this narrative essay, we conclusively analyze the mechanisms of action of CHM antidepressant constituents and formulas, specifically through the modulation of the neuroendocrine-immune network, by reviewing recent preclinical studies conducted using depressive animal models. Some CHM herbal constituents and formulas are highlighted as examples, and their mechanisms of action at both the molecular and systems levels are discussed. Furthermore, we discuss the crosstalk of these two biological systems and the systems pharmacology approach for understanding the system-wide mechanism of action of CHM on the neuroendocrine-immune network in depression treatment. The holistic, multidrug, and multitarget nature of CHM represents an excellent example of systems medicine in the effective treatment of depression.

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