Isolation and characterization of nitrogen fixing heterotrophic bacteria from the rhizosphere of pioneer plants growing on mine tailings

2012 ◽  
Vol 62 ◽  
pp. 52-60 ◽  
Author(s):  
Yendi E. Navarro-Noya ◽  
Emma Hernández-Mendoza ◽  
Jesús Morales-Jiménez ◽  
Janet Jan-Roblero ◽  
Esperanza Martínez-Romero ◽  
...  
Keyword(s):  
Mine Tailings ◽  
Heterotrophic Bacteria ◽  
Nitrogen Fixing ◽  
Isolation And Characterization ◽  
Pioneer Plants

Isolation and characterization of non‐transposon symbiotic nitrogen fixing mutants of Medicago truncatula

2019 ◽  
pp. 1006-1014
Author(s):  
Gyöngyi Zs. Kontra‐Kováts ◽  
Lili Fodor ◽  
Beatrix Horváth ◽  
Ágota Domonkos ◽  
Gergely Iski ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Nitrogen Fixing ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

Microflore hétérotrophe impliquée dans le procédé simultané de biolixiviation des métaux et de digestion des boues d'épuration

2001 ◽  
Vol 28 (1) ◽  
pp. 158-174 ◽  
Author(s):  
M Gamache ◽  
J F Blais ◽  
R D Tyagi ◽  
N Meunier
Keyword(s):  
Sewage Sludge ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Microbial Populations ◽  
Primary Sludge ◽  
Sludge Digestion ◽  
Isolation And Characterization ◽  
Secondary Sludge ◽  
Blastoschizomyces Capitatus

Until now, some microbiological studies have made it possible to highlight the role and identification of certain chimiolithotroph microorganisms directly involved in the simultaneous sewage sludge digestion and metal leaching (SSDML process). Moreover, some other studies have established the performance of the SSDML process for the destruction of pathogens. However, until now no study has been carried out to define the equilibrium of the heterotrophic microbial populations during the sludge acidification occurring during the operation of the SSDML process. Hence, the SSDML process was studied in batch and continuous mode employing different types of sludges (Communauté Urbaine de Québec (CUQ)-East primary sludge, Beauceville secondary sludge, and mixture of primary and secondary Valcartier sludge). The equilibrium of heterotrophic microbial populations was investigated along with sludge acidification and solids degradation. The results obtained during the SSDML process on different sludges showed a decrease followed by the destruction of a majority of heterotrophic bacterial species found in fresh sludges. The isolation and characterization of different microbial species showed that only two types of microorganisms persisted: the yeast Blastoschizomyces capitatus and an unidentified fungus.Key words: sewage sludge, simultanous bioleaching and digestion, heterotrophic bacteria, Blastoschizomyces capitatus, fungi.

scholarly journals Isolation and Characterization of Carbonoclastic Bacteria Diversity in Oil-contaminated Soil in Cape Coast Metropolis, Ghana

2020 ◽  
pp. 62-74
Author(s):  
H. D. Nyarko ◽  
G. C. Okpokwasili ◽  
O. F. Joel ◽  
I. A. K. Galyuon
Keyword(s):  
Petroleum Hydrocarbon ◽  
Heterotrophic Bacteria ◽  
Soil Samples ◽  
Cape Coast ◽  
Bacterial Populations ◽  
Isolation And Characterization ◽  
Sampling Locations ◽  
Degrading Bacteria ◽  
Polluted Sites

Aims: The study aimed at the quantification, isolation and characterization of hydrocarbon degrading bacteria in oil-contaminated and pristine soils. Methodology: Soil samples from petroleum hydrocarbon polluted sites at auto-mechanic workshops, a mechanic village, as well as pristine (control) soils, comprising of 14 sampling locations within Cape Coast Metropolis in the Central Region of Ghana were collected using standard sampling techniques. Collected soil samples were treated and cultured while enumerations, isolations and characterization of carbonoclastic bacteria associated were evaluated. Results: Bacterial populations isolated from hydrocarbon-polluted sites had higher aerobic counts ranging from 7.24-8.02 log10 cfu/g of soil when compared with the pristine sites (from 6.79-7.61 log10 cfu/g of soil). Also, soil samples from the mechanic village (8.76 to 7.48 log10 cfu/g of soil) recorded more bacterial counts than those from the mechanic garages (8.02 to 7.24 log10 cfu/g of soil). The calculated percentage profiles of all the hydrocarbon utilizing bacteria in the total culturable heterotrophic bacteria were low throughout the study, even though the percentage scores were all above 50%. A total of 19 hydrocarbon degraders were isolated. The isolates identified belong to the genera Pseudomonas, Proteus, Bacillus and Enterobacter. Conclusion: The outcome of the study based on the bacteria populations, identification profiles, coupled with their survival and multiplications in designated medium amended with crude oil as the carbon and energy sources, suggest their petroleum hydrocarbon degrading capabilities, hence may be used in bioremediation applications.

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