Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. This study aim to isolate and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, West Java, Indonesia. The water samples were collected along three points around Cilalay Lake. Water physical and chemical determination was performed using the Water Quality Checker. The bacterial isolates were screened on Triptone Glucose Yeast (TGY) agar plates. Afterwards selected isolates were grown on Nutrient Agar media 50% with supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates were resistant. The most abundant type of resistant bacteria to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water

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Pseudomonas and Pedobacter isolates from King George Island inhibited the growth of foodborne pathogens

Polish Polar Research ◽

10.2478/v10183-011-0003-y ◽

2011 ◽

Vol 32 (1) ◽

pp. 3-14 ◽

Cited By ~ 20

Author(s):

Clemente Wong ◽

Heng Tam ◽

Siti Alias ◽

Marcelo González ◽

Gerardo González-Rocha ◽

...

Keyword(s):

Foodborne Pathogens ◽

South Shetland Islands ◽

King George Island ◽

Bacterial Isolates ◽

Inhibitory Effects ◽

Gram Negative ◽

Isolation And Characterization ◽

Protease Treatment ◽

Biochemical Profiles

PseudomonasandPedobacterisolates from King George Island inhibited the growth of foodborne pathogensThis report describes the isolation and characterization of bacterial isolates that produce anti-microbial compounds from one of the South Shetland Islands, King George Island, Antarctica. Of a total 2465 bacterial isolates recovered from the soil samples, six (BG5, MTC3, WEK1, WEA1, MA2 and CG21) demonstrated inhibitory effects on the growth of one or more Gram-negative or Gram-positive indicator foodborne pathogens (i.e. Escherichia coli0157:H7,Salmonellaspp.,Klebsiella pneumoniae, Enterobacter cloacae, Vibrio parahaemolyticusandBacillus cereus). Upon examination of their 16S rRNA sequences and biochemical profiles, the six Antarctic bacterial isolates were identified as Gram-negativePedobacter cryoconitis(BG5),Pseudomonas migulae(WEK1),P. corrugata(WEA1) andPseudomonasspp. (MTC3, MA2, and CG21). While inhibitors produced by strains BG5, MTC3 and CG21 were sensitive to protease treatment, those produced by strains WEK1, WEA1, and MA2 were insensitive to catalase, lipase, α-amylase, and protease enzymes. In addtion, the six Antarctic bacterial isolates appeared to be resistant to multiple antibiotics.

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Isolation and characterization of mineral potassium solubilizing bacteria from rhizosphere soils

Journal of Applied and Natural Science ◽

10.31018/jans.v8i2.861 ◽

2016 ◽

Vol 8 (2) ◽

pp. 705-710 ◽

Cited By ~ 1

Author(s):

Arup Sen ◽

Dhaneshwar Padhan ◽

S.C. Poi

Keyword(s):

West Bengal ◽

Rhizosphere Soil ◽

Gram Negative ◽

Isolation And Characterization ◽

Feasible Option ◽

Agar Media ◽

Beneficial Traits ◽

Potassium Solubilizing Bacteria

Attempts were made to isolate potasium solubilizing bacteria (KSB) from rhizosphere soil of different crops in Nadia district of West Bengal. A total of ten bacteria isolates were tested for K solubilization and characterized up to genus level based on morphological and biochemical characters. Among the ten isolates, six were gram positive rods belonging to genera Bacillus and remaining four isolates was gram negative rod belonging to genera Pseudomonas. The mechanisms involved in K solubilization and other agronomical beneficial traits were also analyzed for selected efficient strains. The diameter of zone of solubilization formed by the isolates on agar media supplemented with mica at 1 per cent ranged from 0.60 to 1.26 cm at 72 hours after incubation. In vitro K solubilization from mica powder by bacteria ranged from 3.23Î¼g/ml to 41.20Î¼g/ml at 20 days after inoculation. Organic acids production by the KSB isolates is the main reason for solubilization of potassium from mica powder. Results further suggested that the use of efficient potassium mobilizers can be a feasible option for meeting the K-requirement of crops.

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Isolation and Characterization of Mercury-Resistant Bacteria From Sediments of Tagus Estuary (Portugal): Implications for Environmental and Human Health Risk Assessment

Journal of Toxicology and Environmental Health Part A ◽

10.1080/15287394.2014.867204 ◽

2014 ◽

Vol 77 (1-3) ◽

pp. 155-168 ◽

Cited By ~ 16

Author(s):

Neusa L. L. Figueiredo ◽

João Canário ◽

Aida Duarte ◽

Maria Luísa Serralheiro ◽

Cristina Carvalho

Keyword(s):

Risk Assessment ◽

Health Risk ◽

Human Health ◽

Health Risk Assessment ◽

Human Health Risk ◽

Tagus Estuary ◽

Resistant Bacteria ◽

Isolation And Characterization ◽

Mercury Resistant Bacteria

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Isolation and characterization of arsenic resistant soil bacteria and their effects on germination of rice under arsenic contamination

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v2i2.25003 ◽

2015 ◽

Vol 2 (2) ◽

pp. 229-237

Author(s):

Istiaq Ahmed ◽

Md Tofazzal Islam ◽

Md Akhter Hossain Chowdhury ◽

Md Kamruzzaman

Keyword(s):

Contaminated Soils ◽

Sodium Arsenite ◽

Agar Plate ◽

Rice Seedling ◽

Resistant Bacteria ◽

Gram Staining ◽

Isolation And Characterization ◽

Resistant Strains

This study was carried out to isolate, screen and characterize arsenic (As) resistant bacteria from As contaminated soils of Dumrakandi and Matlab under Faridpur and Chandpur districts and to evaluate their efficiency in reducing As toxicity against rice seedlings during germination. Thirteen strains were isolated from the soils which showed resistance to different levels of sodium arsenite (viz. 5, 10, 20 and 40 mM) in both agar plate and broth assay using BSMY I media. Among the isolates, BTL0011, BTL0012, BTL0015 and BTL0022 showed highest resistance to 40 mM sodium arsenite. Gram staining and KOH solubility test revealed that five strains were gram positive and rest eight was gram negative. They grew well in the liquid media at pH 5.5 to 8.5. In-vitro rice seedling bioassay with two superior isolates (BTL0011 and BTL0022) revealed that As resistant strains significantly enhanced seed germination of BRRI dhan29 and BRRI dhan47 at 60 ppm As. This study was laid out in CRD with three replications. The performance of BTL 0022 was superior to BTL0011. The overall results suggest that BTL0011 and BTL0022 can be used for bioremediation of As contaminated soils and to increase the germination and seedling growth of rice in As contaminated soils.Res. Agric., Livest. Fish.2(2): 229-237, August 2015

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Diversity and Antibiotic Susceptibility of Bacteria in Water of Hotel Restaurants in Dhaka City

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v43i2.46515 ◽

2017 ◽

Vol 43 (2) ◽

pp. 173-180

Author(s):

Md Abdul Karim ◽

Nasrin Sultana

Keyword(s):

Bacterial Abundance ◽

Bacterial Count ◽

Dhaka City ◽

Bacterial Isolates ◽

Genus Bacillus ◽

Gram Positive ◽

Gram Negative ◽

Agar Media ◽

Micrococcus Roseus ◽

Microbiological Status

Present study was conducted to determine the microbiological status of water from dispensers in different roadside hotel and restaurants of Dhaka city. Samples were collected from seven hotel and restaurants. Aerobic heterotrophic bacterial count ranged between 1.5 × 10 and 8.8 × 103 cfu/ml. Enteric and related bacterial abundance in MacConkey, SS and Cetrimide agar media ranged from 0 to 4.9 × 106, 0 to 2.1 × 105 and 0 to 1.2 × 106cfu/ml, respectively. In total, 28 bacterial isolates were obtained during the study period. Among them, 15 were heterotrophic isolates and 13 were enteric and related bacteria. Among 15 aerobic heterotrophic isolates, 11 were gram positive and five were gram negative. Out of 11 gram positive isolates, 7 belonged to the genus Bacillus viz. B. circulans, B. subtilis, B. stearothermophilus, B. brevis and B. cereus and one to coccus viz. Micrococcus roseus. The other gram positive species were Kurtia gibsonii, Auriobacterium liguefaciens and Curtobacterium luteum. Four gram negative isolates were Neisseria elongate sub. spp. glycolytica, Plesiomonas shigelloides, Pseudomonas fluorescens biovar 1, Pseudomonas aeruginosa. All 13 enteric and related isolates were gram negative, short rod; and non-spore formers and belonged to the genera Escherichia, Klebsiella, Shigella and Pseudomonas. Among all isolates, two were resistant and six were susceptible to all five antibiotics. Asiat. Soc. Bangladesh, Sci. 43(2): 173-180, December 2017

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Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases

International Journal of Molecular Sciences ◽

10.3390/ijms21093160 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3160 ◽

Cited By ~ 1

Author(s):

Pilar Domingo-Calap ◽

Beatriz Beamud ◽

Lucas Mora-Quilis ◽

Fernando González-Candelas ◽

Rafael Sanjuán

Keyword(s):

Klebsiella Pneumoniae ◽

Multidrug Resistant ◽

Health Concern ◽

Resistant Bacteria ◽

Isolation And Characterization ◽

The Family ◽

Important Challenge ◽

Tail Spike ◽

Reference Strains

The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.

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Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia

International Journal of Microbiology ◽

10.1155/2020/8893266 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Mohabaw Jemal ◽

Teshiwal Deress ◽

Teshome Belachew ◽

Yesuf Adem

Keyword(s):

Antimicrobial Resistance ◽

Blood Culture ◽

Agar Plate ◽

Blood Cultures ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Aids Patients ◽

Gram Positive ◽

Gram Negative ◽

Hiv Aids

Background. The emergence and spread of antimicrobial resistance in bacteria is recognized as a global public health problem. Bloodstream infection with antimicrobial-resistant bacteria in HIV/AIDS patients makes the problem more challenging. So, regular and periodic diagnosis and use of the appropriate antimicrobial susceptibility pattern determination is the only option for decreasing the prevalence and development of drug-resistant bacteria. Methods. An institution-based cross-sectional study was conducted among 384 HIV/AIDS patients. Sociodemographic data of patients were recorded using structured questionnaires. Blood cultures were collected with BACTEC aerobic blood culture bottles. A pair of samples was collected from each patient aseptically and incubated at 37°. If samples are positive for bacterial agents, they were subcultured to solid media such as blood agar plate, chocolate agar plate, and MacConkey agar plates. Identification was performed using colony characteristics and standard biochemical techniques. The antimicrobial susceptibility test was determined by the Kirby–Bauer disc diffusion method. Data entry and analysis were performed while using SPSS version 20. Descriptive statistics were performed to calculate frequencies. Results. Altogether, 384 patients were included, and 123 blood cultures were positive, so that the yield was thus 32%. About 46 (37.4%) of Gram-negative and 77 (62.6%) of Gram-positive bacterial species were identified. Among Gram-negative bacterial isolates, K. pneumoniae was the leading pathogen, 19 (41.3%), whereas S. aureus, 38 (49.4%), was predominant among Gram-positive isolates. In his study, the majority of Gram-positive isolates showed high level of resistance to penicillin, 72 (95.5%), tetracycline, 55 (71.4%), and cotrimoxazole, 45 (58.4%). About 28 (73.6%) of S. aureus isolates were also methicillin-resistant. Gram-negative bacterial isolates also showed a high resistance to ampicillin (91.3%), tetracycline (91.3%), and gentamicin (47.8%). Overall, about 78% of multidrug resistance was observed. Conclusion. Several pathogens were resistant to greater than five antimicrobial agents, so that proper management of patients with bacteremia is needed, and a careful selection of effective antibiotics should be practiced.

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THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI

Canadian Journal of Microbiology ◽

10.1139/m64-100 ◽

1964 ◽

Vol 10 (5) ◽

pp. 791-799 ◽

Cited By ~ 41

Author(s):

L. R. Brown ◽

R. J. Strawinski ◽

C. S. McCleskey

Keyword(s):

Formic Acid ◽

Inorganic Nitrogen ◽

Nitrogen Sources ◽

Energy Sources ◽

Resting Cells ◽

Gram Negative ◽

Oxidation Of Methane ◽

Isolation And Characterization ◽

Trapping Agent

Procedures for the isolation and characterization of Metkanomonas methanooxidans Brown and Strawinski are described. Isolates from varied sources are alike in cellular morphology, inasmuch as they form only microcolonies, and in their dependence on methane or methanol as carbon and energy sources for growth. Both organic and inorganic nitrogen sources are used. The organism is a Gram negative non-sporeforming rod, 1.5 to 3.0 μ by 1.0 μ in size, and motile by means of a single polar flagellum. In growing cultures the oxygen/methane ratio was approximately 1.1 and in resting cells 1.7. The R.Q. for methane with resting cells was 0.43. Resting cells were unable to oxidize organic compounds other than methane, methanol, formaldehyde, and formate. Formic acid was detected in test solutions after cell suspensions had metabolized methane, methanol, and formaldehyde. Using sodium sulphite as trapping agent for formaldehyde, it was found that 60 to 70% of the methane or methanol consumed was converted to formaldehyde. In the presence of iodoacetate, 70% of the methane consumed was present terminally as methanol. Thus it was shown that methanol, formaldehyde, and formic acid are sequential intermediates in the oxidation of methane by these organisms.

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