Outbreak of vibriosis caused by Vibrio harveyi and Vibrio alginolyticus in farmed seahorse Hippocampus kuda in China

Aquaculture ◽  
2020 ◽  
Vol 523 ◽  
pp. 735168 ◽  
Author(s):  
Jiasong Xie ◽  
Lingfei Bu ◽  
Shan Jin ◽  
Xinyi Wang ◽  
Qingsong Zhao ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Vibrio Alginolyticus ◽  
Hippocampus Kuda

Selection and Identification of Common Aptamers against Both Vibrio Harveyi and Vibrio Alginolyticus

2020 ◽  
Vol 48 (5) ◽  
pp. 623-631 ◽  
Author(s):  
Hui-Min LIU ◽  
Ju-Min HAO ◽  
Jing XU ◽  
Qing-Pi YAN ◽  
Han GAO ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Vibrio Alginolyticus

scholarly journals Skrining Dan Seleksi Bakteri Simbion Spons Penghasil Enzim Ekstraseluler Sebagai Agen Bioremediasi Bahan Organik Dan Biokontrol Vibriosis Pada Budidaya Udang

2016 ◽  
Vol 19 (1) ◽  
pp. 11 ◽  
Author(s):  
Wilis Arii Setyati ◽  
Ahmad Saddam Habibi ◽  
Subagiyo Subagiyo ◽  
Ali Ridlo ◽  
Nirwani S ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Vibrio Alginolyticus ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Acinetobacter Sp

Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp. Kata kunci:  Bakteri simbion spons, enzim ekstraseluler, bioremediasi, vibriosis

scholarly journals Probiotic Potential of Indigenous (Bacillus sp. RCS1, Pantoea agglomerans RCS2, and Bacillus cereus strain RCS3) Isolated From Cobia Fish (Rachycentron canadum) and Their Antagonistic Effects on the Growth of Pathogenic Vibrio alginolyticus, Vibrio harveyi, Streptococcus iniae, and Streptococcus agalactiae

2021 ◽  
Vol 8 ◽  
Author(s):  
Eric Amenyogbe ◽  
Jian-sheng Huang ◽  
Gang Chen ◽  
Wei-zheng Wang
Keyword(s):  
Bacillus Cereus ◽  
Streptococcus Agalactiae ◽  
Vibrio Harveyi ◽  
Bacterial Species ◽  
Vibrio Alginolyticus ◽  
Pantoea Agglomerans ◽  
Streptococcus Iniae ◽  
Rachycentron Canadum ◽  
Antagonistic Effects ◽  
Wide Range

Large-scale fish farming faces many environmental stresses, which affect their immune systems, growth performance, and physiological homeostasis, resulting in increase in their susceptibility to infections. Some of the most common bacterial infections of cobia fish (Rachycentron canadum) include streptococcosis, vibriosis, furunculosis and mycobacteriosis, and pastelleurosis. Probiotics could be helpful in reducing or limiting the incidence of severe disease infections or outbreaks. Therefore, the present study aimed to isolate the indigenous bacterial species from healthy cobia fish and then selected 3 strains, including Bacillus sp. RCS1 (MW560712), Pantoea agglomerans RCS2 (MW560713), and Bacillus cereus RCS3 (MW560714) from the gut of juvenile’s cobia having advantageous assets or positive characteristics. Their analysis indicated the presence of similar biochemical profiles and all could effectively utilize carbon sources. The biosafety assessment did not show any pathological symptoms after 10 days of injecting the fish with isolated bacteria. The results showed that all the isolated bacteria in the present study had low auto-aggregation capacity within the first 3 h of incubation. The isolated bacteria showed strong tolerance when exposed to a range of pH. Although asymmetrically, a slow rise in the growth of isolated bacteria was observed within the pH range of 1–8 for RC1, 1–7 for RC2, and 1–6 for RC3. The antagonistic effects of isolated bacterial strains on the development of pathogens, including Vibrio alginolyticus, Vibrio harveyi, Streptococcus iniae, and Streptococcus agalactiae, were investigated using Luria-Bertani (LB) agar plates. All the isolated bacteria exhibited inhibitory effects against the pathogens, including V. alginolyticus, V. harveyi, S. iniae, and S. agalactiae. These isolated bacteria were characterized with a wide range of antagonistic activities, non-hemolytic activities, high survivability after heat-treatments and safety confidence, and antibiotic susceptibility. Generally, the characteristics displayed by these strains indicated that they could be used as potential probiotics in the aquaculture industry.

scholarly journals DIAGNOSIS DAN PENGENDALIAN PENYAKIT INFEKSIUS PADA INDUK KUDA LAUT, Hippocampus kuda DI HATCHERY

2018 ◽  
Vol 10 (2) ◽  
pp. 353-364
Author(s):  
Des Roza
Keyword(s):  
Vibrio Alginolyticus ◽  
Cryptocaryon Irritans ◽  
Hippocampus Kuda

Produksi benih kuda laut, Hippocampus kuda di hatchery telah berkembang di Bali Utara. Tujuan penelitian ini untuk mendiagnosis dan pengendalian penyakit infeksius pada induk kuda laut di hatchery. Tetapi masalah yang dihadapi adalah tingginya tingkat kematian induk selama pemeliharaan di hatchery, yang diduga akibat infeksi penyakit. Penelitian ini dilakukan untuk mendiagnosis agen penyakit melalui serangkaian observasi, isolasi, dan identifikasi serta melakukan pengendalian penyakit. Hasil menunjukkan bahwa dari uji MIC memperlihatkan bahwa pertumbuhan bakteri V. alginolyticus dapat dihambat dengan larutan campuran 1 ppm nifurpirinol 10% dan 2,5 ppm vetstrep. Sedangkan penggunaan copper sulfat 0,5 ppm dan formalin 20 ppm mampu mematikan C. irritans, sementara itu acriflavin netral 5 ppm dan formalin 20 ppm efektif membunuh Trichodina sp. Berdasarkan hasil penelitian dapat disimpulkan dari induk kuda laut telah diperoleh satu isolat bakteri Vibrio alginolyticus dan duajenis protozoa Cilliata parasitic, yaitu Cryptocaryon irritans dan Trichodina sp.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK BUAH ADAS (Foeniculum vulgare, Mill) PADA Vibrio harveyi DAN Vibrio alginolyticus Antibacterial Activity of Fennel (Foeniculum vulgare Mill) Extract on Vibrio alginolyticus and Vibrio harveyi

2015 ◽  
Vol 35 (03) ◽  
pp. 266
Author(s):  
Budianto Budianto ◽  
Arief Prajitno ◽  
Ating Yuniarti
Keyword(s):  
Antibacterial Activity ◽  
Vibrio Harveyi ◽  
Water Extract ◽  
Inhibition Zone ◽  
Vibrio Alginolyticus ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Foeniculum Vulgare ◽  
Disk Diffusion Method ◽  
Paper Disk

Evaluation of natural products as a safe and effective antimicrobial agent is a scientific strategy to treat the drugresistant pathogens.Fennel(FoeniculumvulgareMill) is an herbal plant that has an active in gredient which is one of its benefit sasan antibacterial material.In thisstudy,water extract of fennel fruit determined the antibacterial activity against Vibrio harveyi and Vibrio alginolyticus using the minimumInhibitory  Concentration Test (MIC) and paper disk diffusion method. The results obtained on the MIC, the smallest concentration inhibited the growth was 0,060 g/ml, for both bacteria. The variations on Paper Disk Diffusion Method were the concentrations of A (0.065 g/ml), B (0.070g/ml), C (0.075 g/mL), D (0.080 g/ml), E (0.085 g/ml), F (0.090 g/ml) and Control (0.000 g/ml). The concentration of 0.090 g/ml had the highest inhibition zone diameter in 11.17 ± 0.5 mm (V. harveyi) and 12.53 ± 1.14 mm (V. alginolyticus). It can be concluded that the fruits of fennel (F. vulgare Mill) has a very important ecological role as an alternative treatment in controlling the spread of vibriosis caused by V. harveyi and V. alginolyticus.Keywords: Foeniculum vulgare Mill, Vibrio harveyi, Vibrio alginolyticus, MIC test and paper disk diffusionABSTRAKEvaluasi produk alami sebagai agen antimikrobia yang aman dan efektif adalah salah satu strategi ilmiah untuk memerangi ancaman patogen resistan terhadap obat. Fennel, (Foeniculum vulgare Mill), umumnya dikenal sebagai adas, merupakan tanaman herbal yang memiliki bahan aktif yang salah satu manfaatnya adalah sebagai bahan antibakteri.Pada penelitian ini menggunakan ekstrak air dari buah adas untuk mengetahui aktivitas antibakteri terhadap Vibrio harveyi dan Vibrio alginolyticus dengan menggunakan metode uji Minimum Inhibitory Concentration (MIC) dan difusi cakram kertas. Hasil yang diperoleh pada uji MIC, konsentrasi terkecil untuk menghambat pertumbuhan adalah 0,060 g/ml, untuk kedua spesies bakteri. Variasi perlakuan pada uji cakram kertas yaitu konsentrasi A (0,065 g/ml), B (0,070 g/ml), C (0,075 g/ml), D (0,080 g/ml), E (0,085 g/ml), F (0,090 g/ml) dan kontrol (0,000 g/ml), hasil yang diperoleh adalah konsentrasi 0,090 g/ml memiliki diameter zona hambat tertinggi sebesar 11,17 ± 0,5 mm (V. harveyi) dan 12,53 ± 1,14 mm (V. alginolyticus), sehingga dapat disimpulkan bahwa buah adas (F. vulgare Mill) memiliki peranan ekologi yang sangat penting sebagai bahan pengobatan alternatif dalam pengendalian penyebaran penyakit Vibriosis yang disebabkan oleh V. harveyi dan V. alginolyticus.Kata kunci: Foeniculum vulgare Mill, Vibrio harveyi, Vibrio alginolyticus, uji MIC dan difusi cakram kertas 

scholarly journals Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

2008 ◽  
Vol 45 (supl.) ◽  
pp. 40 ◽  
Author(s):  
Celso Carlos Buglione ◽  
Fabíola Pedrotti ◽  
Felipe Do Nascimento Vieira ◽  
Walter Quadros Seifert ◽  
José Luiz Mouriño ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Litopenaeus Vannamei ◽  
Pasteurella Multocida ◽  
Vibrio Harveyi ◽  
Aeromonas Salmonicida ◽  
Vibrio Alginolyticus

Objetivou-se averiguar o efeito da bactéria Lactobacillus plantarum e células inativas das bactérias Vibrio alginolyticus, Aeromonas salmonicida e Pasteurella multocida na sobrevivência larval de Litoenaeus vannamei, no teste de estresse e infecção experimental com Vibrio harveyi. Foram utilizados tanques cônicos de 30 L, povoados com 400 larvas cada, no estádio de pós-larva cinco. Tratamentos em triplicatas foram consistidos de: 1: ração comercial (controle), 2: ração comercial + bacterina via oral na artemia, 3: ração comercial + bacterina via imersão e 4: ração comercial com inóculo de Lactobacillus plantarum. A aplicação da bacterina ocorreu seis horas antes da infecção e do teste de estresse; enquanto o Lactobacillus plantarum foi administrado por 15 dias antes dos desafios. As pós-larvas do tratamento 4 (ração suplementada com L. plantarum) obtiveram maior índice de sobrevivência no teste de estresse (87,86 ± 2,35%), seguido dos tratamentos 2 e 3 (bacterina via imersão e oral) com 81,54±1,50% e 80,16 ± 2,15% respectivamente, superiores ao índice do controle (72,63 ± 3,34%). Já no desafio com V. harveyi, os animais do grupo tratado com a adição de bacterina via imersão apresentaram maior sobrevivência (79,60 ± 7,12%). As pós-larvas dos tratamentos com bacterina via oral na artêmia e alimentadas com o probiótico L. plantarum, apresentaram sobrevivências de 65,60 ± 5,18% e 69,60 ± 10,43 %, respectivamente, sendo superiores ao controle (56,4 ± 5,58%), quando desafiados com V. harveyi. Os resultados demonstram que o uso de ração com L. plantarum e bacterina aumentam a sobrevivência das pós-larvas de L. vannamei frente aos testes de estresse e infecções experimentais com V. harveyi.

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