Selection and Identification of Common Aptamers against Both Vibrio Harveyi and Vibrio Alginolyticus

2020 ◽  
Vol 48 (5) ◽  
pp. 623-631 ◽  
Author(s):  
Hui-Min LIU ◽  
Ju-Min HAO ◽  
Jing XU ◽  
Qing-Pi YAN ◽  
Han GAO ◽  
...  
2016 ◽  
Vol 19 (1) ◽  
pp. 11 ◽  
Author(s):  
Wilis Arii Setyati ◽  
Ahmad Saddam Habibi ◽  
Subagiyo Subagiyo ◽  
Ali Ridlo ◽  
Nirwani S ◽  
...  

Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp. Kata kunci:  Bakteri simbion spons, enzim ekstraseluler, bioremediasi, vibriosis


2021 ◽  
Vol 8 ◽  
Author(s):  
Eric Amenyogbe ◽  
Jian-sheng Huang ◽  
Gang Chen ◽  
Wei-zheng Wang

Large-scale fish farming faces many environmental stresses, which affect their immune systems, growth performance, and physiological homeostasis, resulting in increase in their susceptibility to infections. Some of the most common bacterial infections of cobia fish (Rachycentron canadum) include streptococcosis, vibriosis, furunculosis and mycobacteriosis, and pastelleurosis. Probiotics could be helpful in reducing or limiting the incidence of severe disease infections or outbreaks. Therefore, the present study aimed to isolate the indigenous bacterial species from healthy cobia fish and then selected 3 strains, including Bacillus sp. RCS1 (MW560712), Pantoea agglomerans RCS2 (MW560713), and Bacillus cereus RCS3 (MW560714) from the gut of juvenile’s cobia having advantageous assets or positive characteristics. Their analysis indicated the presence of similar biochemical profiles and all could effectively utilize carbon sources. The biosafety assessment did not show any pathological symptoms after 10 days of injecting the fish with isolated bacteria. The results showed that all the isolated bacteria in the present study had low auto-aggregation capacity within the first 3 h of incubation. The isolated bacteria showed strong tolerance when exposed to a range of pH. Although asymmetrically, a slow rise in the growth of isolated bacteria was observed within the pH range of 1–8 for RC1, 1–7 for RC2, and 1–6 for RC3. The antagonistic effects of isolated bacterial strains on the development of pathogens, including Vibrio alginolyticus, Vibrio harveyi, Streptococcus iniae, and Streptococcus agalactiae, were investigated using Luria-Bertani (LB) agar plates. All the isolated bacteria exhibited inhibitory effects against the pathogens, including V. alginolyticus, V. harveyi, S. iniae, and S. agalactiae. These isolated bacteria were characterized with a wide range of antagonistic activities, non-hemolytic activities, high survivability after heat-treatments and safety confidence, and antibiotic susceptibility. Generally, the characteristics displayed by these strains indicated that they could be used as potential probiotics in the aquaculture industry.


2015 ◽  
Vol 35 (03) ◽  
pp. 266
Author(s):  
Budianto Budianto ◽  
Arief Prajitno ◽  
Ating Yuniarti

Evaluation of natural products as a safe and effective antimicrobial agent is a scientific strategy to treat the drugresistant pathogens.Fennel(FoeniculumvulgareMill) is an herbal plant that has an active in gredient which is one of its benefit sasan antibacterial material.In thisstudy,water extract of fennel fruit determined the antibacterial activity against Vibrio harveyi and Vibrio alginolyticus using the minimumInhibitory  Concentration Test (MIC) and paper disk diffusion method. The results obtained on the MIC, the smallest concentration inhibited the growth was 0,060 g/ml, for both bacteria. The variations on Paper Disk Diffusion Method were the concentrations of A (0.065 g/ml), B (0.070g/ml), C (0.075 g/mL), D (0.080 g/ml), E (0.085 g/ml), F (0.090 g/ml) and Control (0.000 g/ml). The concentration of 0.090 g/ml had the highest inhibition zone diameter in 11.17 ± 0.5 mm (V. harveyi) and 12.53 ± 1.14 mm (V. alginolyticus). It can be concluded that the fruits of fennel (F. vulgare Mill) has a very important ecological role as an alternative treatment in controlling the spread of vibriosis caused by V. harveyi and V. alginolyticus.Keywords: Foeniculum vulgare Mill, Vibrio harveyi, Vibrio alginolyticus, MIC test and paper disk diffusionABSTRAKEvaluasi produk alami sebagai agen antimikrobia yang aman dan efektif adalah salah satu strategi ilmiah untuk memerangi ancaman patogen resistan terhadap obat. Fennel, (Foeniculum vulgare Mill), umumnya dikenal sebagai adas, merupakan tanaman herbal yang memiliki bahan aktif yang salah satu manfaatnya adalah sebagai bahan antibakteri.Pada penelitian ini menggunakan ekstrak air dari buah adas untuk mengetahui aktivitas antibakteri terhadap Vibrio harveyi dan Vibrio alginolyticus dengan menggunakan metode uji Minimum Inhibitory Concentration (MIC) dan difusi cakram kertas. Hasil yang diperoleh pada uji MIC, konsentrasi terkecil untuk menghambat pertumbuhan adalah 0,060 g/ml, untuk kedua spesies bakteri. Variasi perlakuan pada uji cakram kertas yaitu konsentrasi A (0,065 g/ml), B (0,070 g/ml), C (0,075 g/ml), D (0,080 g/ml), E (0,085 g/ml), F (0,090 g/ml) dan kontrol (0,000 g/ml), hasil yang diperoleh adalah konsentrasi 0,090 g/ml memiliki diameter zona hambat tertinggi sebesar 11,17 ± 0,5 mm (V. harveyi) dan 12,53 ± 1,14 mm (V. alginolyticus), sehingga dapat disimpulkan bahwa buah adas (F. vulgare Mill) memiliki peranan ekologi yang sangat penting sebagai bahan pengobatan alternatif dalam pengendalian penyebaran penyakit Vibriosis yang disebabkan oleh V. harveyi dan V. alginolyticus.Kata kunci: Foeniculum vulgare Mill, Vibrio harveyi, Vibrio alginolyticus, uji MIC dan difusi cakram kertas 


Author(s):  
Celso Carlos Buglione ◽  
Fabíola Pedrotti ◽  
Felipe Do Nascimento Vieira ◽  
Walter Quadros Seifert ◽  
José Luiz Mouriño ◽  
...  

Objetivou-se averiguar o efeito da bactéria Lactobacillus plantarum e células inativas das bactérias Vibrio alginolyticus, Aeromonas salmonicida e Pasteurella multocida na sobrevivência larval de Litoenaeus vannamei, no teste de estresse e infecção experimental com Vibrio harveyi. Foram utilizados tanques cônicos de 30 L, povoados com 400 larvas cada, no estádio de pós-larva cinco. Tratamentos em triplicatas foram consistidos de: 1: ração comercial (controle), 2: ração comercial + bacterina via oral na artemia, 3: ração comercial + bacterina via imersão e 4: ração comercial com inóculo de Lactobacillus plantarum. A aplicação da bacterina ocorreu seis horas antes da infecção e do teste de estresse; enquanto o Lactobacillus plantarum foi administrado por 15 dias antes dos desafios. As pós-larvas do tratamento 4 (ração suplementada com L. plantarum) obtiveram maior índice de sobrevivência no teste de estresse (87,86 ± 2,35%), seguido dos tratamentos 2 e 3 (bacterina via imersão e oral) com 81,54±1,50% e 80,16 ± 2,15% respectivamente, superiores ao índice do controle (72,63 ± 3,34%). Já no desafio com V. harveyi, os animais do grupo tratado com a adição de bacterina via imersão apresentaram maior sobrevivência (79,60 ± 7,12%). As pós-larvas dos tratamentos com bacterina via oral na artêmia e alimentadas com o probiótico L. plantarum, apresentaram sobrevivências de 65,60 ± 5,18% e 69,60 ± 10,43 %, respectivamente, sendo superiores ao controle (56,4 ± 5,58%), quando desafiados com V. harveyi. Os resultados demonstram que o uso de ração com L. plantarum e bacterina aumentam a sobrevivência das pós-larvas de L. vannamei frente aos testes de estresse e infecções experimentais com V. harveyi.


2008 ◽  
Vol 7 (2) ◽  
pp. 179
Author(s):  
. Widanarni ◽  
E. Ayuzar ◽  
. Sukenda

<p>Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of <em>Vibrio harveyi</em>. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of <em>V. harveyi</em> was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of <em>V. harveyi</em>, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic). Number of <em>V. harveyi </em>in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture.  It demonstrated possible inhibition of probiotic bacteria on <em>V. harveyi</em> through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to <em>Pseudoalteromonas piscicida</em>, whereas SKT-b and Ua were similar to <em>Vibrio alginolyticus</em>.</p> <p>Keywords: probiotic bacteria, inhibitory mechanism, <em>V. harveyi</em>, tiger shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan<em> V. harveyi </em>secara <em>in vitro</em>. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (Rf<sup>R</sup>) melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu.  Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan <em>V. harveyi</em> diamati melalui pemberian secara bersamaan antara bakteri probiotik dan <em>V. harveyi</em> tersebut dalam air pemeliharaan larva udang.  Jumlah sel bakteri probiotik,<em> V. harveyi</em> dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung.  Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik). Jumlah sel <em>V. harveyi</em> pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap <em>V. harveyi</em> yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies <em>Pseudoalteromonas piscicida, </em>sedangkan SKT-b dan Ua termasuk spesies <em>Vibrio alginolyticus</em>. </p> <p>Kata kunci: bakteri probiotik, mekanisme penghambatan, <em>V. harveyi</em>, udang windu</p>


Aquaculture ◽  
2020 ◽  
Vol 523 ◽  
pp. 735168 ◽  
Author(s):  
Jiasong Xie ◽  
Lingfei Bu ◽  
Shan Jin ◽  
Xinyi Wang ◽  
Qingsong Zhao ◽  
...  

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