scholarly journals Transcriptomic analysis of the immune response against A. hydrophila infection in striped catfish Pangasianodon hypophthalmus

Aquaculture ◽  
2021 ◽  
pp. 737446
Author(s):  
Zhuo-hao Ruan ◽  
Liang-sen Jiang ◽  
Yi-fu Li ◽  
Zhi-qiang Lu ◽  
Xian-can Chen ◽  
...  
2021 ◽  
Vol 20 (2) ◽  
pp. 163-173
Author(s):  
Erni Susanti ◽  
Dinamella Wahjuningrum ◽  
Sri Nuryati ◽  
Mia Setiawati

Striped catfish Pangasianodon hypophthalamus is one of the intensive cultured commodities. Disease outbreak becomes inevitable to prevent in a fish culture. One of the most frequent disease occurred in striped catfish is the motile aeromonas septicemia (MAS) disease caused by Aeromonas hydrophila. This study aimed to evaluate the effectiveness of 1% dietary cinnamon powder and 0.5% dietary cinnamon leaf extract on the immune response of striped catfish challenged with A. hydrophila. Striped catfish used in this study sizing of 5.80 ± 0.21 g. This study contained two phases, namely in vitro and in vivo tests. In vitro test contained inhibition zone and antibacterial tests, which demonstrates that 1% cinnamon powder and 0.5% cinnamon leaf extract are effective to inhibit A. hydrophila activity. In vivo test contained four treatments, i.e fish fed with 1% cinnamon leaf powder supplemented diet; 0.5% cinnamon leaf extract supplemented diet, positive control diet, and negative control diet. Each treatment was performed in three replications. The result showed that 1% cinnamon leaf powder supplemented diet obtained the best results to enhance the immune response of striped catfish higher survival rate value at 83.33% than the positive control diet (P<0.05). Keywords: Aeromonas hydrophila, Cinnamomum burmannii, extract, Pangasianodon hypophthalmus, powder. ABSTRAK Ikan patin Pangasianodon hypophthalamus termasuk komoditas yang banyak dibudidayakan secara intensif. Kendala budidaya seperti penyakit pun sulit untuk dihindari. Salah satu jenis penyakit yang kerap menyerang ikan patin yaitu penyakit MAS (motile aermomonad septicaemia) yang disebabkan oleh Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji efektivitas 1% (w/w) tepung dan 0,5% (w/w) ekstrak daun kayu manis dalam pakan sebagai upaya pencegahan infeksi bakteri A. hydrophila pada ikan patin. Ikan patin yang digunakan berukuran 5,80 ± 0,21 g. Penelitian ini terdiri dua tahap yaitu uji in vitro dan uji in vivo. Hasil uji in vitro terhadap aktivitas antibakteri menunjukkan bahwa dosis 1% (w/w) tepung daun kayu manis dan 0.5% (w/w) ekstrak daun kayu manis efektif dalam menghambat pertumbuhan A. hydrophila. Uji in vivo terdiri atas empat perlakuan yaitu pemberian pakan dengan penambahan tepung daun kayu manis 1% (w/w), pemberian pakan dengan penambahan ekstrak daun kayu manis 0,5% (w/w), kontrol positif, dan kontrol negatif  dengan masing-masing perlakuan terdiri atas tiga ulangan. Hasil penelitian menunjukkan bahwa penambahan 1% (w/w) tepung daun kayu manis dalam pakan memberikan hasil terbaik dalam meningkatkan respons imun ikan patin dengan tingkat kelangsungan hidup sebesar 83,33% lebih tinggi dibandingkan kontrol positif (P<0,05). Kata kunci: Aeromonas hydrophila, Cinnamomum burmannii, ekstrak, Pangasianodon hypophthalmus, tepung


2017 ◽  
Vol 24 (1) ◽  
pp. 616-624 ◽  
Author(s):  
N. Daniel ◽  
A.P. Muralidhar ◽  
P.P. Srivastava ◽  
K.K. Jain ◽  
K. Pani Prasad ◽  
...  

Aquaculture ◽  
2010 ◽  
Vol 306 (1-4) ◽  
pp. 92-100 ◽  
Author(s):  
Tam M. Bui ◽  
Lam T. Phan ◽  
Brett A. Ingram ◽  
Thuy T.T. Nguyen ◽  
Geoff J. Gooley ◽  
...  

2021 ◽  
Vol 20 (1) ◽  
pp. 61-72
Author(s):  
Thawanrat Puttipong ◽  
◽  
Sinlapachai Senarat ◽  
Jes Kettratad ◽  
Chitchai Chantangsi ◽  
...  

Khlong Saen Saep is one of the most important and large canal systems in Bangkok, Thailand. However, the emergence of heavily polluted water has occasionally been reported due to the domestic sewage and industrial effluents discharged into the system. This situation may affect the reproductive status of aquatic lives residing in this canal. To evaluate the reproductive status of the canal inhabitant, the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878)—a common dweller of the Saen Saep Canal—was collected from two selected stations, the Bumpen Nua Temple and the Kamalun Islam Mosque. The fish gonads were then anatomically and histologically examined. The results indicated that neither parasites nor ovary with external abnormality were found. Nevertheless, several histological alterations were detected, including vacuolar degeneration of previtellogenic oocytes, accumulation of melanomacrophage centers (MMCs), and atretic oocytes. Moreover, severe histopathological changes, such as the eosinophilic cytoplasm of spermatogonia, MMCs, blood congestion, and syncytium of spermatozoa, were observed in the fish testis. Our study suggested that the environmental stress and pollutions previously reported from the Saen Saep Canal possibly underpin the gonadal impairments of P. hypophthalmus examined and may have adverse impact on the reproductive health of others living in this canal system


Sign in / Sign up

Export Citation Format

Share Document