Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

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Streptococcus Mutans Strains in Mother-Child Pairs of Children with Early Childhood Caries

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-43.4.5 ◽

2019 ◽

Vol 43 (4) ◽

pp. 252-256 ◽

Cited By ~ 2

Author(s):

Priya Subramaniam ◽

Revathy Suresh

Keyword(s):

Early Childhood ◽

Dental Caries ◽

Streptococcus Mutans ◽

Early Childhood Caries ◽

Mutans Streptococci ◽

Similar Distribution ◽

Healthy Children ◽

Only Children ◽

Polymerase Chain ◽

Childhood Caries

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

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Effects of antibacterial photodynamic therapy on salivary mutans streptococci in 5- to 6-year-olds with severe early childhood caries

Lasers in Medical Science ◽

10.1007/s10103-018-2650-2 ◽

2018 ◽

Vol 34 (3) ◽

pp. 433-440 ◽

Cited By ~ 4

Author(s):

Majid Bargrizan ◽

Reza Fekrazad ◽

Niloofar Goudarzi ◽

Nastaran Goudarzi

Keyword(s):

Early Childhood ◽

Photodynamic Therapy ◽

Early Childhood Caries ◽

Mutans Streptococci ◽

Severe Early Childhood Caries ◽

Childhood Caries

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Comparison between Clinical Aspects and Salivary Microbial Profile of Children with and without Early Childhood Caries: A Preliminary Study

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-39.3.209 ◽

2015 ◽

Vol 39 (3) ◽

pp. 209-214 ◽

Cited By ~ 10

Author(s):

AB Neves ◽

LA Lobo ◽

KC Pinto ◽

ES Pires ◽

MEP Requejo ◽

...

Keyword(s):

Early Childhood ◽

Microbial Diversity ◽

Early Childhood Caries ◽

Clinical Aspects ◽

Candida Spp ◽

Culture Methods ◽

Microbial Profile ◽

Saliva Collection ◽

Lactobacillus Spp ◽

Childhood Caries

Purpose: To compare the clinical aspects and microbial profile of children with and without early childhood caries (ECC). Study design: 14 patients (7 without caries and 7 with ECC) were submitted to anamnesis, clinical exam and saliva collection for microbiological analyses. Counts of Streptococcus mutans, Lactobacillus spp. Candida spp., and total microorganisms were performed by culture methods. Microbial diversity was characterized by PCR-DGGE. Demographic/clinical data and salivary microbial counts were compared between groups. Results: Habits of hygiene and breastfeeding presented no association with ECC. Use of pacifiers was associated with absence of caries (p=0.035). Counts of total microorganisms and Candida spp. did not differ between the groups. The ECC group presented larger quantity of S. mutans (p=0.026) and Lactobacillus spp. (p=0.002). There was no correlation between microorganisms and breastfeeding and pacifier use. The dmf-t of ECC Group was 10.5±1.9 and the modified dmf-t was 11.3±3.6. The DGGE demonstrated difference in the pattern of bands between the groups. Conclusion: Pacifiers usage was associated with the absence of ECC and microorganism number was higher in the caries group. The PCR-DGGE revealed a characteristic microbial diversity in the ECC Group, being an excellent tool for observing the dynamics of the salivary microbial community in these patients.

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Can insoluble polysaccharide concentration in dental plaque, sugar exposure and cariogenic microorganisms predict early childhood caries? A follow-up study

Archives of Oral Biology ◽

10.1016/j.archoralbio.2015.04.003 ◽

2015 ◽

Vol 60 (8) ◽

pp. 1091-1097 ◽

Cited By ~ 11

Author(s):

T.M. Parisotto ◽

R. Stipp ◽

L.K.A. Rodrigues ◽

R.O. Mattos-Graner ◽

L.S. Costa ◽

...

Keyword(s):

Early Childhood ◽

Dental Plaque ◽

Early Childhood Caries ◽

Follow Up Study ◽

Childhood Caries

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Early Childhood Caries: Epidemiology, Aetiology, and Prevention

International Journal of Dentistry ◽

10.1155/2018/1415873 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 23

Author(s):

F. Meyer ◽

J. Enax

Keyword(s):

Early Childhood ◽

Dental Plaque ◽

Early Childhood Caries ◽

Oral Care ◽

Rapid Progression ◽

Poor Oral Hygiene ◽

Caries Epidemiology ◽

Oral Microorganisms ◽

Childhood Caries

Early childhood caries (ECC) is one of the most prevalent diseases in children worldwide. ECC is driven by a dysbiotic state of oral microorganisms mainly caused by a sugar-rich diet. Additionally, poor oral hygiene or insufficient dental plaque removal leads to the rapid progression of ECC. ECC leads not only to dental destruction and pain with children, but also affects the quality of life of the caregivers. Children with extensive ECC are at high risk to develop caries with the permanent dentition or will have other problems with speaking and/or eating. To prevent ECC, several strategies should be taken into account. Children should brush their teeth with toothpastes containing gentle ingredients, such as mild surfactants and agents showing antiadherent properties regarding oral microorganisms. Parents/caregivers have to help their children with brushing the teeth. Furthermore, remineralizing and nontoxic agents should be included into the toothpaste formulation. Two promising biomimetic agents for children’s oral care are amorphous calcium phosphate [Cax(PO4)ynH2O] and hydroxyapatite [Ca5(PO4)3(OH)].

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Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽

10.12688/f1000research.16275.2 ◽

2018 ◽

Vol 7 ◽

pp. 1645 ◽

Cited By ~ 5

Author(s):

Endang W. Bachtiar ◽

Boy M. Bachtiar

Keyword(s):

Early Childhood ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Dental Plaque ◽

Early Childhood Caries ◽

Positive Correlation ◽

Unstimulated Saliva ◽

Student’S T ◽

Total Bacteria ◽

Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

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