Comparison between Clinical Aspects and Salivary Microbial Profile of Children with and without Early Childhood Caries: A Preliminary Study

Purpose: To compare the clinical aspects and microbial profile of children with and without early childhood caries (ECC). Study design: 14 patients (7 without caries and 7 with ECC) were submitted to anamnesis, clinical exam and saliva collection for microbiological analyses. Counts of Streptococcus mutans, Lactobacillus spp. Candida spp., and total microorganisms were performed by culture methods. Microbial diversity was characterized by PCR-DGGE. Demographic/clinical data and salivary microbial counts were compared between groups. Results: Habits of hygiene and breastfeeding presented no association with ECC. Use of pacifiers was associated with absence of caries (p=0.035). Counts of total microorganisms and Candida spp. did not differ between the groups. The ECC group presented larger quantity of S. mutans (p=0.026) and Lactobacillus spp. (p=0.002). There was no correlation between microorganisms and breastfeeding and pacifier use. The dmf-t of ECC Group was 10.5±1.9 and the modified dmf-t was 11.3±3.6. The DGGE demonstrated difference in the pattern of bands between the groups. Conclusion: Pacifiers usage was associated with the absence of ECC and microorganism number was higher in the caries group. The PCR-DGGE revealed a characteristic microbial diversity in the ECC Group, being an excellent tool for observing the dynamics of the salivary microbial community in these patients.

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Longitudinal Evaluation of Salivary Iga-S in Children with Early Childhood Caries Before and After Restorative Treatment

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-43.4.3 ◽

2019 ◽

Vol 43 (4) ◽

pp. 239-243 ◽

Cited By ~ 2

Author(s):

Aline dos Santos Letieri ◽

Liana Bastos Freitas-Fernandes ◽

Ana Paula Canedo Valente ◽

Tatiana Kelly da Silva Fidalgo ◽

Ivete Pomarico Ribeiro de Souza

Keyword(s):

Early Childhood ◽

Streptococcus Mutans ◽

Flow Rate ◽

Total Protein ◽

Early Childhood Caries ◽

Dental Treatment ◽

Restorative Treatment ◽

Lactobacillus Spp ◽

Childhood Caries

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s > 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p<0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p>0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p<0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p>0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.

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Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries

Archives of Oral Biology ◽

10.1016/j.archoralbio.2006.06.001 ◽

2006 ◽

Vol 51 (11) ◽

pp. 1024-1028 ◽

Cited By ~ 110

Author(s):

Fabíola Galbiatti de Carvalho ◽

Danilo Souza Silva ◽

Josimeri Hebling ◽

Luis Carlos Spolidorio ◽

Denise Madalena Palomari Spolidorio

Keyword(s):

Early Childhood ◽

Dental Plaque ◽

Early Childhood Caries ◽

Mutans Streptococci ◽

Candida Spp ◽

Carious Teeth ◽

Childhood Caries

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Lactobacillus spp. associated with early childhood caries

Folia Microbiologica ◽

10.1007/s12223-009-0008-7 ◽

2009 ◽

Vol 54 (1) ◽

pp. 53-58 ◽

Cited By ~ 16

Author(s):

P. Švec ◽

I. Sedláček ◽

L. Žáčková ◽

D. Nováková ◽

M. Kukletová

Keyword(s):

Early Childhood ◽

Early Childhood Caries ◽

Lactobacillus Spp ◽

Childhood Caries

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Prevalence of Early Childhood Caries among Children at Two Community Health Centers in Hawaii

Journal of Dentistry & Oral Disorders ◽

10.26420/jdentoraldisord.2017.1072 ◽

2017 ◽

Vol 3 (5) ◽

Author(s):

Jennifer L. Logigian

Keyword(s):

Early Childhood ◽

Community Health ◽

Community Health Centers ◽

Early Childhood Caries ◽

Health Centers ◽

Childhood Caries

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Prevention and Treatment of Early Childhood Caries (ECC)

Journal Of Medicine & Health ◽

10.28932/jmh.v1i3.525 ◽

2016 ◽

Vol 1 (3) ◽

Author(s):

Jeffrey .

Keyword(s):

Early Childhood ◽

Health Problem ◽

Early Childhood Caries ◽

Health Providers ◽

Missing Teeth ◽

Optimal Basis ◽

Major Health Problem ◽

Major Health ◽

Childhood Caries

Early Childhood Caries (ECC) is a chronic disease that can be prevented. It commonlyaffects children involving in one or more decayed (with lesions or not) teeth, missing teeth (dueto caries), or teeth with fillings in children aged under 71 months. The disease is sometimesoverlooked, but this condition usually affects the general health of children. Early detection ofEarly Childhood Caries (ECC) can prevent problems which are harmful to children. Therefore,the ECC must be prevented and for teeth that have had dental caries they should be givenproper treatment so as not to worsen and affect the quality of life in children. Prevention of thisdisease is a significant component in any health program to prepare for the optimal basis forthe oral health of children. This condition will become a serious health problem if not handledproperly, and it is a major health problem for health providers throughout the world.Primarypreventive must be initiated since a woman getting pregnant.Keywords: Early Childhood Caries (ECC), prevention, treatment

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