Combinatorial effect of lower extremity blood flow restriction and low intensity endurance exercise on aorta of old male rats: Histomorphological and molecular approach

Objectives: Blood flow restriction (BFR) therapy consists of low-intensity exercise performed under reduced venous return due to an inflatable tourniquet. This produces similar physiologic and clinical effects to high-intensity routines with less joint and tissue stress. Postoperative patients may benefit from more efficient rehabilitation. Proximal and distal effects of BFR have been evaluated, however, minimal literature exists on its use in orthopaedic conditions. The purpose of this study was to determine the effects of low-intensity BFR therapy both proximal and distal, in the upper and lower extremities. Methods: This was a prospective, randomized controlled trial of healthy subjects completing a standardized 6-week course of BFR therapy. Subjects were randomized to BFR therapy on one extremity or to a control group. Subjects were excluded for cardiac, pulmonary, or hematologic disease, pregnancy, or previous surgery in the extremity. Data collected at baseline and completion included: limb circumferences, isokinetic, and manual strength testing. Results: Forty subjects completed the protocol. Average age was 27.7 years; 54% were female. For both upper and lower extremity groups, a statistically significant increase was observed in manual and isokinetic strength both proximal and distal to the BFR tourniquet when compared to both the non-tourniquet extremity and the control group (p<0.05). Limb circumference significantly increased in the upper (p<0.01) and lower extremities (p=0.02). A significant increase in manual strength was noted in shoulder abduction and scaption, and hip extension and abduction even in the non-tourniquet BFR extremity compared to the control group (p<0.05). Conclusion: Low-intensity BFR therapy led to greater increases in muscle strength and hypertrophy. Similar strengthening effects were seen in proximal and distal muscle groups. Strength increases in the contralateral BFR extremity may corroborate a systemic effect. This study provides data to further evaluate the efficacy and safety of BFR therapy in operative and non-operative orthopaedic conditions. [Table: see text]

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HIGH-INTENSITY ENDURANCE EXERCISE INCREASES MORE MUSCLE PGC1-α mRNA EXPRESSION THAN LOW-INTENSITY ENDURANCE EXERCISE WITH BLOOD FLOW RESTRICTION

10.19146/pibic-2015-37326 ◽

2015 ◽

Author(s):

Mara Patricia Traina Chacon Mikahil ◽

Guilherme Defante Telles

Keyword(s):

Blood Flow ◽

Mrna Expression ◽

Endurance Exercise ◽

High Intensity ◽

Blood Flow Restriction ◽

Flow Restriction ◽

Low Intensity

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Muscle oxygenation, endocrine and metabolic regulation during low-intensity endurance exercise with blood flow restriction

The Journal of Exercise Nutrition and Biochemistry ◽

10.20463/pan.2020.0012 ◽

2020 ◽

Vol 24 (2) ◽

pp. 30-37 ◽

Cited By ~ 1

Author(s):

Hyejung Hwang ◽

Sahiro Mizuno ◽

Nobukazu Kasai ◽

Chihiro Kojima ◽

Daichi Sumi ◽

...

Keyword(s):

Blood Flow ◽

Endurance Exercise ◽

Metabolic Regulation ◽

Blood Flow Restriction ◽

Muscle Oxygenation ◽

Flow Restriction ◽

Low Intensity

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Effects of single bouts of different endurance exercises with different intensities on microRNA biomarkers with and without blood flow restriction: a three-arm, randomized crossover trial

European Journal of Applied Physiology ◽

10.1007/s00421-021-04786-2 ◽

2021 ◽

Author(s):

Johanna Sieland ◽

Daniel Niederer ◽

Tobias Engeroff ◽

Lutz Vogt ◽

Christian Troidl ◽

...

Keyword(s):

Heart Rate ◽

Blood Flow ◽

Endurance Exercise ◽

Mirna Expression ◽

Expression Patterns ◽

Lactate Concentration ◽

Significant Negative Correlation ◽

Blood Flow Restriction ◽

Flow Restriction ◽

Low Intensity

Abstract Purpose Physical activity is associated with altered levels of circulating microRNAs (ci-miRNAs). Changes in miRNA expression have great potential to modulate biological pathways of skeletal muscle hypertrophy and metabolism. This study was designed to determine whether the profile of ci-miRNAs is altered after different approaches of endurance exercise. Methods Eighteen healthy volunteers (aged 24 ± 3 years) participated this three-arm, randomized-balanced crossover study. Each arm was a single bout of treadmill-based acute endurance exercise at (1) 100% of the individual anaerobic threshold (IANS), (2) at 80% of the IANS and (3) at 80% of the IANS with blood flow restriction (BFR). Load-associated outcomes (fatigue, feeling, heart rate, and exhaustion) as well as acute effects (circulating miRNA patterns and lactate) were determined. Results All training interventions increased the lactate concentration (LC) and heart rate (HR) (p < 0.001). The high-intensity intervention (HI) resulted in a higher LC than both lower intensity protocols (p < 0.001). The low-intensity blood flow restriction (LI-BFR) protocol led to a higher HR and higher LC than the low-intensity (LI) protocol without BFR (p = 0.037 and p = 0.003). The level of miR-142-5p and miR-197-3p were up-regulated in both interventions without BFR (p < 0.05). After LI exercise, the expression of miR-342-3p was up-regulated (p = 0.038). In LI-BFR, the level of miR-342-3p and miR-424-5p was confirmed to be up-regulated (p < 0.05). Three miRNAs and LC show a significant negative correlation (miR-99a-5p, p = 0.011, r = − 0.343/miR-199a-3p, p = 0.045, r = − 0.274/miR-125b-5p, p = 0.026, r = − 0.302). Two partial correlations (intervention partialized) showed a systematic impact of the type of exercise (LI-BFR vs. HI) (miR-99a-59: r = − 0.280/miR-199a-3p: r = − 0.293). Conclusion MiRNA expression patterns differ according to type of activity. We concluded that not only the intensity of the exercise (LC) is decisive for the release of circulating miRNAs—as essential is the type of training and the oxygen supply.

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