scholarly journals Effects of single bouts of different endurance exercises with different intensities on microRNA biomarkers with and without blood flow restriction: a three-arm, randomized crossover trial

2021 ◽  
Author(s):  
Johanna Sieland ◽  
Daniel Niederer ◽  
Tobias Engeroff ◽  
Lutz Vogt ◽  
Christian Troidl ◽  
...  
Keyword(s):  
Heart Rate ◽  
Blood Flow ◽  
Endurance Exercise ◽  
Mirna Expression ◽  
Expression Patterns ◽  
Lactate Concentration ◽  
Significant Negative Correlation ◽  
Blood Flow Restriction ◽  
Flow Restriction ◽  
Low Intensity

Abstract Purpose Physical activity is associated with altered levels of circulating microRNAs (ci-miRNAs). Changes in miRNA expression have great potential to modulate biological pathways of skeletal muscle hypertrophy and metabolism. This study was designed to determine whether the profile of ci-miRNAs is altered after different approaches of endurance exercise. Methods Eighteen healthy volunteers (aged 24 ± 3 years) participated this three-arm, randomized-balanced crossover study. Each arm was a single bout of treadmill-based acute endurance exercise at (1) 100% of the individual anaerobic threshold (IANS), (2) at 80% of the IANS and (3) at 80% of the IANS with blood flow restriction (BFR). Load-associated outcomes (fatigue, feeling, heart rate, and exhaustion) as well as acute effects (circulating miRNA patterns and lactate) were determined. Results All training interventions increased the lactate concentration (LC) and heart rate (HR) (p < 0.001). The high-intensity intervention (HI) resulted in a higher LC than both lower intensity protocols (p < 0.001). The low-intensity blood flow restriction (LI-BFR) protocol led to a higher HR and higher LC than the low-intensity (LI) protocol without BFR (p = 0.037 and p = 0.003). The level of miR-142-5p and miR-197-3p were up-regulated in both interventions without BFR (p < 0.05). After LI exercise, the expression of miR-342-3p was up-regulated (p = 0.038). In LI-BFR, the level of miR-342-3p and miR-424-5p was confirmed to be up-regulated (p < 0.05). Three miRNAs and LC show a significant negative correlation (miR-99a-5p, p = 0.011, r = − 0.343/miR-199a-3p, p = 0.045, r = − 0.274/miR-125b-5p, p = 0.026, r = − 0.302). Two partial correlations (intervention partialized) showed a systematic impact of the type of exercise (LI-BFR vs. HI) (miR-99a-59: r = − 0.280/miR-199a-3p: r = − 0.293). Conclusion MiRNA expression patterns differ according to type of activity. We concluded that not only the intensity of the exercise (LC) is decisive for the release of circulating miRNAs—as essential is the type of training and the oxygen supply.

scholarly journals Effects on the Profile of Circulating miRNAs after Single Bouts of Resistance Training with and without Blood Flow Restriction—A Three-Arm, Randomized Crossover Trial

2019 ◽  
Vol 20 (13) ◽  
pp. 3249 ◽  
Author(s):  
Johanna Vogel ◽  
Daniel Niederer ◽  
Tobias Engeroff ◽  
Lutz Vogt ◽  
Christian Troidl ◽  
...  
Keyword(s):  
Heart Rate ◽  
Blood Flow ◽  
Resistance Training ◽  
Lactate Concentration ◽  
Blood Flow Restriction ◽  
Collateral Flow ◽  
Circulating Mirnas ◽  
Flow Restriction ◽  
Flexion Extension ◽  
The Individual

Background: The effects of blood flow restriction (training) may serve as a model of peripheral artery disease. In both conditions, circulating micro RNAs (miRNAs) are suggested to play a crucial role during exercise-induced arteriogenesis. We aimed to determine whether the profile of circulating miRNAs is altered after acute resistance training during blood flow restriction (BFR) as compared with unrestricted low- and high-volume training, and we hypothesized that miRNA that are relevant for arteriogenesis are affected after resistance training. Methods: Eighteen healthy volunteers (aged 25 ± 2 years) were enrolled in this three-arm, randomized-balanced crossover study. The arms were single bouts of leg flexion/extension resistance training at (1) 70% of the individual single-repetition maximum (1RM), (2) at 30% of the 1RM, and (3) at 30% of the 1RM with BFR (artificially applied by a cuff at 300 mm Hg). Before the first exercise intervention, the individual 1RM (N) and the blood flow velocity (m/s) used to validate the BFR application were determined. During each training intervention, load-associated outcomes (fatigue, heart rate, and exhaustion) were monitored. Acute effects (circulating miRNAs, lactate) were determined using pre-and post-intervention measurements. Results: All training interventions increased lactate concentration and heart rate (p < 0.001). The high-intensity intervention (HI) resulted in a higher lactate concentration than both lower-intensity training protocols with BFR (LI-BFR) and without (LI) (LI, p = 0.003; 30% LI-BFR, p = 0.008). The level of miR-143-3p was down-regulated by LI-BFR, and miR-139-5p, miR-143-3p, miR-195-5p, miR-197-3p, miR-30a-5p, and miR-10b-5p were up-regulated after HI. The lactate concentration and miR-143-3p expression showed a significant positive linear correlation (p = 0.009, r = 0.52). A partial correlation (intervention partialized) showed a systematic impact of the type of training (LI-BFR vs. HI) on the association (r = 0.35 remaining after partialization of training type). Conclusions: The strong effects of LI-BFR and HI on lactate- and arteriogenesis-associated miRNA-143-3p in young and healthy athletes are consistent with an important role of this particular miRNA in metabolic processes during (here) artificial blood flow restriction. BFR may be able to mimic the occlusion of a larger artery which leads to increased collateral flow, and it may therefore serve as an external stimulus of arteriogenesis.

Combinatorial effect of lower extremity blood flow restriction and low intensity endurance exercise on aorta of old male rats: Histomorphological and molecular approach

2018 ◽  
Vol 24 (C) ◽  
pp. 22 ◽  
Author(s):  
Mohammad-Abbas Bejeshk ◽  
Siyavash Joukar ◽  
Beydolah Shahouzehi ◽  
Majid Asadi-shekari ◽  
Mohammadamin Rajizadeh ◽  
...  
Keyword(s):  
Blood Flow ◽  
Lower Extremity ◽  
Endurance Exercise ◽  
Blood Flow Restriction ◽  
Male Rats ◽  
Molecular Approach ◽  
Flow Restriction ◽  
Low Intensity

Pressor Responses To High And Low-intensity Continuous Or Interval Cycling With/without Blood Flow Restriction.

2017 ◽  
Vol 49 (5S) ◽  
pp. 254
Author(s):  
Bianca Galletti ◽  
Francisco Batista ◽  
Jarbas Domingos-Gomes ◽  
Eduardo Freitas ◽  
Piettra Pereira ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Flow Restriction ◽  
Flow Restriction ◽  
Low Intensity ◽  
Pressor Responses ◽  
Interval Cycling

Effects of Active Preconditioning With Local and Systemic Hypoxia on Submaximal Cycling

2021 ◽  
pp. 1-6
Author(s):  
Olivier Girard ◽  
Romain Leuenberger ◽  
Sarah J. Willis ◽  
Fabio Borrani ◽  
Grégoire P. Millet
Keyword(s):  
Blood Flow ◽  
Arterial Oxygen Saturation ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Blood Flow Restriction ◽  
Arterial Oxygen ◽  
Total Occlusion ◽  
Flow Restriction ◽  
Cycling Efficiency ◽  
Systemic Hypoxia

Purpose: The authors compared the effects of active preconditioning with local and systemic hypoxia during submaximal cycling. Methods: On separate visits, 14 active participants completed 4 trials. Each visit was composed of 1 preconditioning phase followed, after 40 minutes of rest, by 3 × 6-minute cycling bouts (intensity = 85% of critical power; rest = 6 min). The preconditioning phase consisted of 4 × 5-minute cycling bouts at 1.5 W·kg−1 (rest = 5 min) in 4 conditions: control (no occlusion and normoxia), blood flow restriction (60% of total occlusion), HYP (systemic hypoxia; inspired fraction of oxygen = 13.6%), and blood flow restriction + HYP (local and systemic hypoxia combined). Results: During the preconditioning phase, there were main effects of both systemic (all P < .014) and local hypoxia (all P ≤ .001) on heart rate, arterial oxygen saturation, leg discomfort, difficulty of breathing, and blood lactate concentration. Cardiorespiratory variables, gross efficiency, energy cost, and energy expenditure during the last minute of 6-minute cycling bouts did not differ between conditions (all P > .105). Conclusion: Local and systemic hypoxic stimuli, or a combination of both, during active preconditioning did not improve physiological responses such as cycling efficiency during subsequent submaximal cycling.

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