Pathogenicity of entomopathogenic fungus, Metarhizium anisopliae MET-GRA4 isolate on dengue vectors, Aedes albopictus and Aedes aegypti mosquito larvae (Diptera: Culicidae)

2021 ◽  
Author(s):  
Wan Fatma Zuharah ◽  
Md Rodzay Rohaiyu ◽  
Wahizatul Afzan Azmi ◽  
Hideyuki Nagao
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Mosquito Larvae ◽  
Dengue Vectors

A Simple PCR Assay for Discrimination of Dengue Vectors from Nsukka LGA, Nigeria

2020 ◽  
Vol 10 (1) ◽  
pp. 67-77
Author(s):  
Amos Watentena ◽  
Ikem Chris Okoye ◽  
Ikechukwu Eugene Onah ◽  
Onwude Cosmas Ogbonnaya ◽  
Emmanuel Ogudu
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Mosquito Species ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Mosquito Vector ◽  
Aedes Mosquitoes ◽  
Allele Specific ◽  
Arboviral Diseases ◽  
Dengue Vectors

Mosquitoes of Aedes species are vectors of several arboviral diseases which continue to be a major public health problem in Nigeria. This study among other things, morphologically identified Aedes mosquitoes collected from Nsukka LGA and used an allele specific PCR amplification for discrimination of dengue vectors. Larval sampling, BG-sentinel traps and modified human landing catches were used for mosquito sampling in two selected autonomous communities of Nsukka LGA (Nsukka and Obimo). A total of 124 Aedes mosquitoes consisting of five (5) different species were collected from April to June, 2019 in a cross-sectional study that covered 126 households, under 76 distinct geographical coordinates. Larvae was mainly collected from plastic containers 73% (n=224), metallic containers 14% (n=43), earthen pots 9% (n=29) and used car tyres 3% (n=9), reared to adult stage 69.35% (n=86), and all mosquitoes were identified using standard morphological keys. Five (5) Aedes mosquito species were captured; Aedes aegypti 83(66.94%), Aedes albopictus 33(26.61%), Aedes simpsoni (4.48%), Aedes luteocephalus (≤1%) and Aedes vittatus (≤1%). Nsukka autonomous community had higher species diversity than Obimo. Allele specific amplification confirmed dengue vectors, Aedes aegypti and Aedes albopictus species on a 2% agarose gel. Since the most recent re-emergence of arboviral diseases is closely associated with Aedes species, findings of this study, therefore, give further evidence about the presence of potential arboviral vectors in Nigeria and describe the role of a simple PCR in discriminating some. Further entomological studies should integrate PCR assays in mosquito vector surveillance.

scholarly journals Oviposition Habitat Selection of Dengue Vectors, Aedes aegypti and Aedes albopictus in Response to Fish Predator

2016 ◽  
Vol 27 (3) ◽  
pp. 117-122 ◽  
Author(s):  
Wan Fatma Zuharah ◽  
◽  
Nik Fadzly ◽  
Wilson Kang Wei Ong ◽  
Zarul Hazrin Hashim ◽  
...  
Keyword(s):  
Habitat Selection ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Fish Predator ◽  
Dengue Vectors ◽  
Selection Of

Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti

2012 ◽  
Vol 104 (2) ◽  
pp. 126-131 ◽  
Author(s):  
J. Vontas ◽  
E. Kioulos ◽  
N. Pavlidi ◽  
E. Morou ◽  
A. della Torre ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Aedes Albopictus ◽  
Dengue Vectors

scholarly journals Insecticide resistance of Dengue vectors in South East Asia: a systematic review

2021 ◽  
Vol 21 (3) ◽  
pp. 1124-1140
Author(s):  
Mohd Rohaizat Hassan ◽  
Noor Atika Azit ◽  
Suhaiza Mohd Fadzil ◽  
Siti Rasidah Abd Ghani ◽  
Norfazilah Ahmad ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
East Asia ◽  
Aedes Albopictus ◽  
Pyrethroid Resistance ◽  
Resistance Management ◽  
Genetic Mutation ◽  
Peninsular Malaysia ◽  
South East Asia ◽  
Dengue Vectors

Background: The insecticides used widely has led to resistance in the vector and impose a challenge to vector control op- eration. Objectives: This review aims to analyse the distribution of insecticide resistance of dengue vectors in South East Asia and to describe the mechanism of insecticide resistance. Methods: Literature search for articles published on 2015 to 2019 from PubMed, Scopus and ProQuest was performed. Total of 37 studies included in the final review from the initial 420 studies. Results: Pyrethroid resistance was concentrated on the west coast of Peninsular Malaysia and Northern Thailand and scat- tered at Java Island, Indonesia while organophosphate resistance was seen across the Java Island (Indonesia), West Sumatera and North Peninsular Malaysia. Organochlorine resistance was seen in Sabah, Malaysia and scattered distribution in Nusa Tenggara, Indonesia. V1016G, S989P, F1269C gene mutation in Aedes Aegypti were associated with Pyrethroid resistance in Singapore and Indonesia. In Malaysia, over-expressed with monooxygenase P450 genes (CYP9J27, CYP6CB1, CYP9J26 and CYP9M4) Glutathione S-transferases, carboxylesterases commonly associated with pyrethroids resistance in Aedes Aegypti and CYP612 overexpressed in Aedes Albopictus. The genetic mutation in A302S in Aedes Albopictus was associated with organochlorine resistance in Malaysia. Conclusions: Rotation of insecticide, integration with synergist and routine assessment of resistance profile are recom- mended strategies in insecticide resistance management. Keywords: Insecticide resistance; vector management; Aedes; pyrethroid; mortality.

scholarly journals Neem oil increases the persistence of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Adriano R. Paula ◽  
Anderson Ribeiro ◽  
Francisco José Alves Lemos ◽  
Carlos P. Silva ◽  
Richard I. Samuels
Keyword(s):  
Aedes Aegypti ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Neem Oil

Infection of adult Aedes aegypti and Ae. albopictus mosquitoes with the entomopathogenic fungus Metarhizium anisopliae

Acta Tropica ◽  
2007 ◽  
Vol 102 (3) ◽  
pp. 151-158 ◽  
Author(s):  
Ernst-Jan Scholte ◽  
Willem Takken ◽  
Bart G.J. Knols
Keyword(s):  
Aedes Aegypti ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus
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