scholarly journals Smart Drain for Post Cardiac Surgery Left Ventricular Volumes Evaluated in Large Animal Models

2021 ◽  
Author(s):  
Aleksandra B. Gruslova ◽  
Andrew G. Cabe ◽  
Anil Kottam ◽  
John Walmsley ◽  
John E. Porterfield ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Animal Models ◽  
Left Ventricular ◽  
Left Ventricular Volumes ◽  
Large Animal ◽  
Ventricular Volumes ◽  
Large Animal Models ◽  
Post Cardiac Surgery

scholarly journals Comparative Analysis of Methods to Induce Myocardial Infarction in a Closed-Chest Rabbit Model

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Marc-Antoine Isorni ◽  
Amaury Casanova ◽  
Julie Piquet ◽  
Valérie Bellamy ◽  
Charly Pignon ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Models ◽  
Rabbit Model ◽  
Left Ventricular ◽  
Balloon Occlusion ◽  
Gelatin Sponge ◽  
Large Animal ◽  
Alcohol Injection ◽  
Closed Chest ◽  
Large Animal Models

Objective.To develop a rabbit model of closed-chest catheter-induced myocardial infarction.Background.Limitations of rodent and large animal models justify the search for clinically relevant alternatives.Methods.Microcatheterization of the heart was performed in 47 anesthetized 3-4 kg New Zealand rabbits to test five techniques of myocardial ischemia: free coils (n=4), interlocking coils (n=4), thrombogenic gelatin sponge (n=4), balloon occlusion (n=4), and alcohol injection (n=8). In order to limit ventricular fibrillation, an antiarrhythmic protocol was implemented, with beta-blockers/amiodarone before and xylocaine infusion during the procedure. Clinical, angiographic, and echographic data were gathered. End points included demonstration of vessel occlusion (TIMI flow grades 0 and 1 on the angiogram), impairment of left ventricular function at 2 weeks after procedure (by echocardiography), and pathologically confirmed myocardial infarction.Results.The best arterial access was determined to be through the right carotid artery. The internal mammary guiding catheter 4-Fr was selected as the optimal device for selective intracoronary injection. Free coils deployed prematurely and tended to prolapse into the aorta. Interlocking coils did not deploy completely and failed to provide reliable results. Gelatin sponge was difficult to handle, adhered to the catheter, and could not be clearly visualized by fluoroscopy. Balloon occlusion yielded inconsistent results. Alcohol injection was the most efficient and reproducible method for inducing myocardial infarction (4 out of 6 animals), the extent of which could be fine-tuned by using a coaxial balloon catheter as a microcatheter (0.52 mm) to achieve a superselective injection of 0.2 mL of alcohol. This approach resulted in a 20% decrease in LVEF and infarcted myocardium was confirmed histologically.Conclusions.By following a stepwise approach, a minimally invasive, effective, and reproducible rabbit model of catheter-induced myocardial infarction has been developed which addresses the limitations of rodent experiments while avoiding the logistical and cost issues associated with large animal models.

Large Animal Models for Left Ventricular Assist Device Research and Development

ASAIO Journal ◽  
2014 ◽  
Vol 60 (1) ◽  
pp. 2-8 ◽  
Author(s):  
Gretel Monreal ◽  
Leslie C. Sherwood ◽  
Michael A. Sobieski ◽  
Guruprasad A. Giridharan ◽  
Mark S. Slaughter ◽  
...  
Keyword(s):  
Animal Models ◽  
Left Ventricular Assist Device ◽  
Ventricular Assist Device ◽  
Left Ventricular ◽  
Large Animal ◽  
Assist Device ◽  
Ventricular Assist ◽  
Large Animal Models ◽  
Left Ventricular Assist ◽  
Device Research

scholarly journals A New Form of Decellularized Extracellular Matrix Hydrogel for Treating Ischemic Tissue via Intravascular Infusion

2020 ◽  
Author(s):  
Martin T. Spang ◽  
Tori S. Lazerson ◽  
Saumya Bhatia ◽  
James Corbitt ◽  
Gerardo Sandoval ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Left Ventricular ◽  
Large Animal Model ◽  
Left Ventricular Volumes ◽  
Large Animal ◽  
Proof Of Concept ◽  
Large Animal Models ◽  
Ischemic Tissue ◽  
New Form

AbstractExtracellular matrix (ECM) hydrogels have been widely used in preclinical studies as injectable materials for tissue engineering therapies. We have developed a new ECM therapy, the soluble fraction derived from decellularized, digested ECM, for intravascular infusion. This new form of ECM is capable of gelation in vivo and can be delivered acutely after an injury to promote cell survival and improve vascularization. In this study, we show proof-of-concept for the feasibility, safety, and efficacy of ECM infusions using small and large animal models of acute myocardial infarction (MI) and intracoronary infusion. Following infusion, the ECM material was retained in the heart, specifically in regions of ischemia, and colocalized with endothelial cells, coating the leaky microvasculature. Functional improvements, specifically reduced left ventricular volumes, were observed after ECM infusion post-MI. Genes associated with angiogenesis were upregulated, and genes associated with cell apoptosis/necrosis and fibrosis were downregulated. The ECM was also delivered using a clinically-relevant catheter in a large animal model of acute MI. This study shows proof-of-concept for a new intravascular delivery strategy for ECM biomaterial therapies with potential implications for a variety of pathologies with ischemic tissue or injured vasculature.

scholarly journals Large Animal Models for Investigating Cell Therapies of Stress Urinary Incontinence

2021 ◽  
Vol 22 (11) ◽  
pp. 6092
Author(s):  
Bastian Amend ◽  
Niklas Harland ◽  
Jasmin Knoll ◽  
Arnulf Stenzl ◽  
Wilhelm K. Aicher
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Animal Models ◽  
Clinical Situation ◽  
Surgical Instruments ◽  
Health Concern ◽  
Large Animal ◽  
Knock Out ◽  
Cell Therapies ◽  
Large Animal Models

Stress urinary incontinence (SUI) is a significant health concern for patients affected, impacting their quality of life severely. To investigate mechanisms contributing to SUI different animal models were developed. Incontinence was induced under defined conditions to explore the pathomechanisms involved, spontaneous recovery, or efficacy of therapies over time. The animal models were coined to mimic known SUI risk factors such as childbirth or surgical injury. However, animal models neither reflect the human situation completely nor the multiple mechanisms that ultimately contribute to the pathogenesis of SUI. In the past, most SUI animal studies took advantage of rodents or rabbits. Recent models present for instance transgenic rats developing severe obesity, to investigate metabolic interrelations between the disorder and incontinence. Using recombinant gene technologies, such as transgenic, gene knock-out or CRISPR-Cas animals may narrow the gap between the model and the clinical situation of patients. However, to investigate surgical regimens or cell therapies to improve or even cure SUI, large animal models such as pig, goat, dog and others provide several advantages. Among them, standard surgical instruments can be employed for minimally invasive transurethral diagnoses and therapies. We, therefore, focus in this review on large animal models of SUI.

A new method for determination of postmortem left ventricular volumes: clinico-pathologic correlations

1975 ◽  
Vol 89 (5) ◽  
pp. 625-628 ◽  
Author(s):  
R.W. Wissler ◽  
C. Lichtig ◽  
R. Hughes ◽  
J. Al-Sadir ◽  
S. Glagov
Keyword(s):  
Left Ventricular ◽  
New Method ◽  
Left Ventricular Volumes ◽  
Ventricular Volumes

Estimation of left ventricular volumes using a simplified method

1976 ◽  
Vol 91 (3) ◽  
pp. 399-400 ◽  
Author(s):  
Eduardo Meaney ◽  
Jacques St-Pierre ◽  
Ralph Shabetai ◽  
André Davignon
Keyword(s):  
Left Ventricular ◽  
Left Ventricular Volumes ◽  
Ventricular Volumes ◽  
Simplified Method
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