scholarly journals Functional characterization of a dodecaheme c-type cytochrome involved in microbial electron transfer to energy production in MFCs

2014 ◽  
Vol 1837 ◽  
pp. e81
Author(s):  
Mónica N. Alves ◽  
Ricardo O. Louro ◽  
Carlos A. Salgueiro ◽  
Catarina M. Paquete
Keyword(s):  
Electron Transfer ◽  
Energy Production ◽  
Functional Characterization

On the road to improve the bioremediation and electricity-harvesting skills of Geobacter sulfurreducens: functional and structural characterization of multihaem cytochromes

2012 ◽  
Vol 40 (6) ◽  
pp. 1295-1301 ◽  
Author(s):  
Leonor Morgado ◽  
Ana P. Fernandes ◽  
Joana M. Dantas ◽  
Marta A. Silva ◽  
Carlos A. Salgueiro
Keyword(s):  
Electron Transfer ◽  
Functional Characterization ◽  
Cost Effective ◽  
Genetically Engineered ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer ◽  
The Road ◽  
On The Road ◽  
Haem Proteins

Extracellular electron transfer is one of the physiological hallmarks of Geobacter sulfurreducens, allowing these bacteria to reduce toxic and/or radioactive metals and grow on electrode surfaces. Aiming to functionally optimize the respiratory electron-transfer chains, such properties can be explored through genetically engineered strains. Geobacter species comprise a large number of different multihaem c-type cytochromes involved in the extracellular electron-transfer pathways. The functional characterization of multihaem proteins is particularly complex because of the coexistence of several microstates in solution, connecting the fully reduced and oxidized states. NMR spectroscopy has been used to monitor the stepwise oxidation of each individual haem and thus to obtain information on each microstate. For the structural study of these proteins, a cost-effective isotopic labelling of the protein polypeptide chains was combined with the comparative analysis of 1H-13C HSQC (heteronuclear single-quantum correlation) NMR spectra obtained for labelled and unlabelled samples. These new methodological approaches allowed us to study G. sulfurreducens haem proteins functionally and structurally, revealing functional mechanisms and key residues involved in their electron-transfer capabilities. Such advances can now be applied to the design of engineered haem proteins to improve the bioremediation and electricity-harvesting skills of G. sulfurreducens.

scholarly journals Functional characterization of mutants deficient in N-terminal phosphorylation of the CF1 subunit beta

2021 ◽  
Author(s):  
Ralph Bock ◽  
Deserah D Strand ◽  
Daniel Karcher ◽  
Stephanie Ruf ◽  
Anne Schadach ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electron Transport ◽  
Atp Synthase ◽  
Electron Transport Chain ◽  
Atp Hydrolysis ◽  
Molecular Motor ◽  
Functional Characterization ◽  
Phosphorylation Sites ◽  
Transport Chain

Understanding the regulation of photosynthetic light harvesting and electron transfer is of great importance to efforts to improve the ability of the electron transport chain to supply downstream metabolism. The central regulator of the electron transport chain is the ATP synthase, the molecular motor that harnesses the chemiosmotic potential generated from proton coupled electron transport to synthesize ATP. The ATP synthase is regulated both thermodynamically and post-translationally, with proposed phosphorylation sites on multiple subunits. In this study we focused on two N-terminal serines on the catalytic subunit beta, previously proposed to be important for dark inactivation of the complex to avoid ATP hydrolysis at night. Here we show that there is no clear role for phosphorylation in the dark inactivation of ATP synthase. Instead, mutation of one of the two phosphorylated serine residues to aspartate strongly decreased ATP synthase abundance. We propose that the loss of N-terminal phosphorylation of ATP beta may be involved in proper ATP synthase accumulation during complex assembly.

scholarly journals Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacteriumGeobacter sulfurreducens

FEBS Letters ◽  
2013 ◽  
Vol 587 (16) ◽  
pp. 2662-2668 ◽  
Author(s):  
Joana M. Dantas ◽  
Diogo M. Tomaz ◽  
Leonor Morgado ◽  
Carlos A. Salgueiro
Keyword(s):  
Electron Transfer ◽  
Functional Characterization

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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