scholarly journals HIV protease inhibitors and onset of cardiovascular diseases: A central role for oxidative stress and dysregulation of the ubiquitin–proteasome system

Author(s):  
Kathleen M.S.E. Reyskens ◽  
M. Faadiel Essop
2016 ◽  
Vol 39 (4) ◽  
pp. 1463-1470 ◽  
Author(s):  
Paola Maura Tricarico ◽  
Rafael Freitas de Oliveira Franca ◽  
Sabrina Pacor ◽  
Valentina Ceglia ◽  
Sergio Crovella ◽  
...  

Background: Prophylactic treatment regimens to prevent mother-to-child HIV transmission include protease inhibitors Lopinavir and Ritonavir. Lopinavir and Ritonavir have been reported to be able to induce intracellular oxidative stress in diverse cellular models, however scarce informations are available about protease inhibitor effects of in the central nervous system (CNS). In our study we evaluated the impact of protease inhibitors on a cell neuronal model. Methods: We treated a neuroblastoma cell line (SH-SY5Y) with increasing doses of Lopinavir and Ritonavir (0.1-1-10-25-50 µM), used alone or in combination, evaluating the impact of these drugs in terms of mitochondrial activity, with MTT cell proliferation assay; mRNA expression of heme oxygenase (HemeOH) and reactive oxygen species (ROS) levels with 2',7'-dichlorofluorescin diacetate (H2DCFDA) in order to assess oxidative stress; apoptotic cell death with flow cytometry. Results: We observed that Lopinavir and Ritonavir treatment, at 25 and/or 50 µM concentrations, induced mitochondrial damage, increase of heme oxygenase RNA expression levels and ROS generation, followed by apoptosis in SH-SY5Y. Conclusions: Our in vitro model demonstrates a damaging effect of HIV protease inhibitors on the neuroblastoma cell line, thus partially mimicking the impact of these drugs on the CNS of children born to HIV positive mothers undergone to antiretroviral treatment.


2004 ◽  
Vol 4 (2) ◽  
pp. 137-152 ◽  
Author(s):  
Jana Prejdova ◽  
Milan Soucek ◽  
Jan Konvalinka

2010 ◽  
Vol 6 (4) ◽  
pp. 269-282 ◽  
Author(s):  
Subhash C. Basak ◽  
Denise Mills ◽  
Rajni Garg ◽  
Barun Bhhatarai

1995 ◽  
Vol 5 (5) ◽  
pp. 459-464 ◽  
Author(s):  
G.S. Bisacchi ◽  
S. Ahmad ◽  
M. Alam ◽  
A. Ashfaq ◽  
J. Barrish ◽  
...  

1996 ◽  
Vol 6 (23) ◽  
pp. 2847-2852 ◽  
Author(s):  
Xiaoqi Chen ◽  
Lin Li ◽  
Dale J. Kempf ◽  
Hing Sham ◽  
Norman E. Wideburg ◽  
...  

2009 ◽  
Vol 52 (3) ◽  
pp. 737-754 ◽  
Author(s):  
Robert N. Jorissen ◽  
G. S. Kiran Kumar Reddy ◽  
Akbar Ali ◽  
Michael D. Altman ◽  
Sripriya Chellappan ◽  
...  

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