Characterization of Ca2+ signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development

DNA methylation reprogramming, the erasure of DNA methylation patterns shortly after fertilization and their reestablishment during subsequent early development, is essential for proper mammalian embryogenesis. In contrast, the importance of this process in the development of non-mammalian vertebrates such as fish is less clear. Indeed, whether or not any widespread changes in DNA methylation occur at all during cleavage and blastula stages of fish in a fashion similar to that shown in mammals has remained controversial. Here we have addressed this issue by applying the techniques of Southwestern immunoblotting and immunohistochemistry with an anti–5-methylcytosine antibody to the examination of DNA methylation in early zebrafish embryos. These techniques have recently been utilized to demonstrate that development-specific changes in genomic DNA methylation also occur in Drosophila melanogaster and Dictyostelium discoideum , both organisms for which DNA methylation was previously not thought to occur. Our data demonstrate that genome-wide changes in DNA methylation occur during early zebrafish development. Although zebrafish sperm DNA is strongly methylated, the zebrafish genome is not detectably methylated through cleavage and early blastula stages but is heavily remethylated in blastula and early gastrula stages.

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Characterization of the carbonic anhydrases 15b expressed in PGCs during early zebrafish development

Theriogenology ◽

10.1016/j.theriogenology.2012.10.016 ◽

2013 ◽

Vol 79 (3) ◽

pp. 443-452 ◽

Cited By ~ 9

Author(s):

H. Wang ◽

Y. Teng ◽

Y. Xie ◽

B. Wang ◽

Y. Leng ◽

...

Keyword(s):

Carbonic Anhydrases ◽

Zebrafish Development

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Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development

Gene Expression Patterns ◽

10.1016/j.modgep.2005.10.007 ◽

2006 ◽

Vol 6 (5) ◽

pp. 546-555 ◽

Cited By ~ 38

Author(s):

Laura A. Hale ◽

Alexandra Tallafuss ◽

Yi-Lin Yan ◽

Leana Dudley ◽

Judith S. Eisen ◽

...

Keyword(s):

Retinoic Acid ◽

Retinoic Acid Receptor ◽

Acid Receptor ◽

Zebrafish Development

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Characterization of vascular mural cells during zebrafish development

Mechanisms of Development ◽

10.1016/j.mod.2009.06.1080 ◽

2009 ◽

Vol 126 (8-9) ◽

pp. 638-649 ◽

Cited By ~ 81

Author(s):

Massimo M. Santoro ◽

Gabriella Pesce ◽

Didier Y. Stainier

Keyword(s):

Zebrafish Development ◽

Mural Cells

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Characterization of the metabolic conditions of different tissues during Zebrafish development by non-linear microscopy

18th Italian National Conference on Photonic Technologies (Fotonica 2016) ◽

10.1049/cp.2016.0951 ◽

2016 ◽

Author(s):

C. Giubani ◽

R. Mercatelli ◽

F. Vanzi ◽

R. Cicchi ◽

F.S. Pavone

Keyword(s):

Zebrafish Development ◽

Non Linear ◽

Metabolic Conditions ◽

Different Tissues

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Characterization of oestrogen receptors in zebrafish (Danio rerio)

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0280153 ◽

2002 ◽

Vol 28 (3) ◽

pp. 153-163 ◽

Cited By ~ 98

Author(s):

PL Bardet ◽

B Horard ◽

M Robinson-Rechavi ◽

V Laudet ◽

JM Vanacker

Keyword(s):

Danio Rerio ◽

Time Windows ◽

Oestrogen Receptors ◽

Rnase Protection ◽

Zebrafish Development ◽

Phylogenetic Studies ◽

Time Periods ◽

Beta 2 ◽

Er Alpha

We cloned the cDNAs corresponding to three oestrogen receptors (ERs) in zebrafish (Danio rerio). Sequence analysis and phylogenetic studies demonstrated that two of these genes, ER beta.1 and ER beta.2, arose from duplication of the original ER beta in many species of the fish phylum, whereas ER alpha is unique. Zebrafish ERs behaved as oestrogen-dependent transcription factors in transactivation assays. However, their reactivity to various oestrogen modulators was different compared with that of mouse ERs. ER mRNA expression during zebrafish development is restricted to distinct time periods, as observed by RNase protection assays. ER beta.2 is initially expressed as maternally transmitted RNA, until 6 h after fertilization, when expression disappears. Between 6 and 48 h after fertilization, no ER expression could be observed. After 48 h after fertilization, all ERs, but predominantly ER alpha, began to be expressed. We conclude that oestrogen signal transduction can operate during zebrafish development only within discrete time windows.

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