Quality Of Umbilical Cord Blood Units Stored For Autologous Use And Infused Intravenously In Children With Acquired Neurological Conditions

Purpose: To assess the association of specific newborn and maternal factors with indicators of increased blood-forming capacity in umbilical cord blood to inform strategic collection strategies that could augment the quality of units in public cord blood banks. Methods: Data regarding 268 consecutive cord blood units (CBUs) banked by Canadian Blood Services were analyzed. Multivariate analysis was performed to identify factors associated with markers of hematopoietic potency and likelihood of utilization. Results: Delayed clamping of the cord beyond 60 s was associated with reduced volume collected. Any delay in clamping of the cord was associated with reduced total nucleated cell counts. Newborn weight >4,000 g was also associated with greater blood volume in the collection but not with other measures of hematopoietic potency. Cord blood acidosis at birth (pH

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High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation

Journal of Clinical Medicine ◽

10.3390/jcm10020293 ◽

2021 ◽

Vol 10 (2) ◽

pp. 293

Author(s):

Gee-Hye Kim ◽

Jihye Kwak ◽

Sung Hee Kim ◽

Hee Jung Kim ◽

Hye Kyung Hong ◽

...

Keyword(s):

Stem Cell ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Clinical Applications ◽

Hematopoietic Stem ◽

Cd34 Cells ◽

Hsc Transplantation

Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units: total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage.

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Quality of Umbilical Cord Blood CD34 + Cells in a Double-Compartment Freezing Bag Cryopreserved without a Rate-Controlled Programmed Freezer

International Journal of Hematology ◽

10.1532/ijh97.06147 ◽

2007 ◽

Vol 85 (1) ◽

pp. 78-84 ◽

Cited By ~ 8

Author(s):

Masayoshi Minegishi ◽

Tsuneo Itoh ◽

Narumi Fukawa ◽

Tamie Kitaura ◽

Junko Miura ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Cd34 Cells ◽

Cord Blood Cd34 ◽

Rate Controlled ◽

Double Compartment

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Abstract 489: Buoyant targeted microbubble technology provides efficient and superior quality of hematopoietic stem cells isolated from umbilical cord blood

10.1158/1538-7445.am2020-489 ◽

2020 ◽

Author(s):

Guixin Shi ◽

Christina C.N. Wu ◽

Sean Darmadi ◽

Zhiyong Wang ◽

Dennis Carson ◽

...

Keyword(s):

Stem Cells ◽

Hematopoietic Stem Cells ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Hematopoietic Stem

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Closing the loop on the quality of umbilical cord blood gas sampling at time of birth

Journal of Obstetrics and Gynaecology ◽

10.1080/718591766 ◽

2003 ◽

Vol 23 (sup1) ◽

pp. S56-S56

Author(s):

M. Harris ◽

K. R. Greene

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Gas ◽

Gas Sampling ◽

Closing The Loop

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Influence of volume reduction and cryopreservation methodologies on quality of thawed umbilical cord blood units for transplantation

Cryobiology ◽

10.1016/j.cryobiol.2008.02.002 ◽

2008 ◽

Vol 56 (2) ◽

pp. 152-158 ◽

Cited By ~ 18

Author(s):

Pilar Solves ◽

Vicente Mirabet ◽

Dolores Planelles ◽

Francisco Carbonell-Uberos ◽

Roberto Roig

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Volume Reduction

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ETHICAL ASPECTS ABOUT INFORMED CONSENT IN CORD BLOOD COLLECTION FOR DIAGNOSTIC PURPOSES

Romanian Journal of Pediatrics ◽

10.37897/rjp.2015.3.4 ◽

2015 ◽

Vol 64 (3) ◽

pp. 259-262

Author(s):

Elena Tataranu ◽

◽

Maria Stamatin ◽

Smaranda Diaconescu ◽

Angelica Cristina Marin ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Care Program ◽

Blood Collection ◽

The Subject ◽

Cord Blood Collection ◽

Allergic March ◽

Specific Care

Deepening the knowledge about genetic and epigenetic mechanisms studies (environmental caused) regarding atopy, brought revelations about intrauterine onset of allergies, proven by the presence of IgE in umbilical cord blood. Detection of these newborns and their inclusion in a specific care program further reduces the morbidity of allergic march with the undeniable effects on quality of life and healthcare costs. The dramatic increase in the prevalence of atopy in recent decades suggests the importance of IgE in umbilical cord determinations. The use of umbilical cord blood for diagnostic purposes requires clear laws and rules and this is the subject we suggest to debate in this paper.

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Does the time between collecting and processing umbilical cord blood samples affect the quality of the sample?

Einstein (São Paulo) ◽

10.1590/s1679-45082011gs1809 ◽

2011 ◽

Vol 9 (2) ◽

pp. 207-211 ◽

Cited By ~ 4

Author(s):

Ricardo Barini ◽

Ubirajara Costa Ferraz ◽

Gregório Lorenzo Acácio ◽

Isabela Nelly Machado

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Linear Correlation ◽

Time Interval ◽

Blood Samples ◽

Viable Cells ◽

Cd34 Cells ◽

The Difference

Objective: To assess the association between the time from umbilical cord blood collection until processing and the quality of the sample. Methods: Umbilical cord blood samples collected during the third stage of labor were placed in temperature-controlled boxes for the transport of biological material and sent to an umbilical cord blood bank, where the number of nucleated cells, viable cells and CD34+ cells were counted, and samples were additionally tested for contamination at the following time intervals: up to 24 hours, up to 48 hours and up to 72 hours following sampling. Data were analyzed using the multivariate analysis of variance (MANOVA) and compared using McNemar's χ2 test. Significance was defined at p < 0.05. Results: Means and medians of the number of nucleated cells, viable cells and CD34+ cells decreased significantly (p < 0.0001) as a function of the increased time between sampling and analysis, the difference between 24 and 48 hours being less than the difference between 24 and 72 hours. A linear correlation was found between the mean number of viable cells and CD34+ cells at the three moments of analysis. Contamination testing was negative in all samples. Conclusion: The increase in time interval from sampling until analysis negatively affected the number of nucleated cells, viable cells and CD34+ cells but was not associated with specimen contamination. A linear correlation was found between decrease in the number of viable cells and CD34+ cells.

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