Does the time between collecting and processing umbilical cord blood samples affect the quality of the sample?

Objective: To assess the association between the time from umbilical cord blood collection until processing and the quality of the sample. Methods: Umbilical cord blood samples collected during the third stage of labor were placed in temperature-controlled boxes for the transport of biological material and sent to an umbilical cord blood bank, where the number of nucleated cells, viable cells and CD34+ cells were counted, and samples were additionally tested for contamination at the following time intervals: up to 24 hours, up to 48 hours and up to 72 hours following sampling. Data were analyzed using the multivariate analysis of variance (MANOVA) and compared using McNemar's χ2 test. Significance was defined at p < 0.05. Results: Means and medians of the number of nucleated cells, viable cells and CD34+ cells decreased significantly (p < 0.0001) as a function of the increased time between sampling and analysis, the difference between 24 and 48 hours being less than the difference between 24 and 72 hours. A linear correlation was found between the mean number of viable cells and CD34+ cells at the three moments of analysis. Contamination testing was negative in all samples. Conclusion: The increase in time interval from sampling until analysis negatively affected the number of nucleated cells, viable cells and CD34+ cells but was not associated with specimen contamination. A linear correlation was found between decrease in the number of viable cells and CD34+ cells.

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High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation

Journal of Clinical Medicine ◽

10.3390/jcm10020293 ◽

2021 ◽

Vol 10 (2) ◽

pp. 293

Author(s):

Gee-Hye Kim ◽

Jihye Kwak ◽

Sung Hee Kim ◽

Hee Jung Kim ◽

Hye Kyung Hong ◽

...

Keyword(s):

Stem Cell ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Clinical Applications ◽

Hematopoietic Stem ◽

Cd34 Cells ◽

Hsc Transplantation

Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units: total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage.

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Quality of Umbilical Cord Blood CD34 + Cells in a Double-Compartment Freezing Bag Cryopreserved without a Rate-Controlled Programmed Freezer

International Journal of Hematology ◽

10.1532/ijh97.06147 ◽

2007 ◽

Vol 85 (1) ◽

pp. 78-84 ◽

Cited By ~ 8

Author(s):

Masayoshi Minegishi ◽

Tsuneo Itoh ◽

Narumi Fukawa ◽

Tamie Kitaura ◽

Junko Miura ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Cd34 Cells ◽

Cord Blood Cd34 ◽

Rate Controlled ◽

Double Compartment

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Isolation of nucleated cells fraction from umbilical cord blood – choice of method

Cell and Organ Transplantology ◽

10.22494/cot.v2i1.34 ◽

2014 ◽

Vol 2 (1) ◽

pp. 30-33 ◽

Cited By ~ 1

Author(s):

D. Ivolgin ◽

A. Smolyaninov

Keyword(s):

Cost Effectiveness ◽

Cell Viability ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Automatic Method ◽

Cost Effectiveness Ratio ◽

Blood Samples ◽

Cd34 Cells ◽

The Cost

The comparison of different techniques for cord blood processing to ensure the highest cell yield based on the “cost-effectiveness” ratio is important.A study of the effectiveness of nucleated cells fraction isolation from umbilical cord blood samples (n = 2898) using double centrifugation, automatic (Sepax S100, Biosafe) or semi-automatic (MacoPress Smart, MacoPharma) system was performed. These include the determining of nucleated cells number (per ml and total), the number of CD34+ cells, and cell viability before samples treatment, after treatment and before freezing.It was shown that semi-automatic and automatic processing methods were more efficient then the double centrifugation by key quality indicators of cell concentrates: the total number of nucleated cells and nucleated cells output. The semi-automatic method of nucleated cells fraction isolation requires the least time for processing a single sample.Considering these data such a system has been proposed as optimal for daily receipt and processing of cord blood samples in a cord blood bank.

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Early apoptosis in CD34+cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood

British Journal of Haematology ◽

10.1111/j.1365-2141.2006.06270.x ◽

2006 ◽

Vol 135 (2) ◽

pp. 210-213 ◽

Cited By ~ 23

Author(s):

Jae-Seung Shim ◽

Bin Cho ◽

Myungshin Kim ◽

Gyeong-Sin Park ◽

Jong-Chul Shin ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Cd34 Cells ◽

Early Apoptosis

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Obstetrical and neonatal factors associated with optimal public banking of umbilical cord blood in the context of delayed cord clamping

Clinical & Investigative Medicine ◽

10.25011/cim.v42i3.33093 ◽

2019 ◽

Vol 42 (3) ◽

pp. E56-E63 ◽

Cited By ~ 1

Author(s):

Anna Munro ◽

Daniel J. Corsi ◽

Lisa Martin ◽

Michael Halpenny ◽

Nicholas Dibdin ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Delayed Cord Clamping ◽

Factors Associated ◽

Cell Counts ◽

Cord Clamping ◽

Newborn Weight ◽

Cord Blood Banks

Purpose: To assess the association of specific newborn and maternal factors with indicators of increased blood-forming capacity in umbilical cord blood to inform strategic collection strategies that could augment the quality of units in public cord blood banks. Methods: Data regarding 268 consecutive cord blood units (CBUs) banked by Canadian Blood Services were analyzed. Multivariate analysis was performed to identify factors associated with markers of hematopoietic potency and likelihood of utilization. Results: Delayed clamping of the cord beyond 60 s was associated with reduced volume collected. Any delay in clamping of the cord was associated with reduced total nucleated cell counts. Newborn weight >4,000 g was also associated with greater blood volume in the collection but not with other measures of hematopoietic potency. Cord blood acidosis at birth (pH

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Hoechst dye efflux reveals a novel CD7+CD34− lymphoid progenitor in human umbilical cord blood

Blood ◽

10.1182/blood.v96.6.2125 ◽

2000 ◽

Vol 96 (6) ◽

pp. 2125-2133 ◽

Cited By ~ 101

Author(s):

Robert W. Storms ◽

Margaret A. Goodell ◽

Alan Fisher ◽

Richard C. Mulligan ◽

Clay Smith

Keyword(s):

Stem Cells ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Large Population ◽

Lymphoid Cells ◽

Human Umbilical Cord Blood ◽

Human Umbilical Cord ◽

Hematopoietic Stem ◽

Cd34 Cells

Abstract A novel Hoechst 33342 dye efflux assay was recently developed that identifies a population of hematopoietic cells termed side population (SP) cells. In the bone marrow of multiple species, including mice and primates, the SP is composed primarily of CD34−cells, yet has many of the functional properties of hematopoietic stem cells (HSCs). This report characterizes SP cells from human umbilical cord blood (UCB). The SP in unfractionated UCB was enriched for CD34+ cells but also contained a large population of CD34− cells, many of which were mature lymphocytes. SP cells isolated from UCB that had been depleted of lineage-committed cells (Lin− UCB) contained CD34+ and CD34− cells in approximately equivalent proportions. Similar to previous descriptions of human HSCs, the CD34+Lin− SP cells were CD38dimHLA-DRdimThy-1dimCD45RA−CD71−and were enriched for myelo-erythroid precursors. In contrast, the CD34−Lin− SP cells were CD38−HLA-DR−Thy-1−CD71−and failed to generate myelo-erythroid progeny in vitro. The majority of these cells were CD7+CD11b+CD45RA+, as might be expected of early lymphoid cells, but did not express other lymphoid markers. The CD7+CD34−Lin− UCB SP cells did not proliferate in simple suspension cultures but did differentiate into natural killer cells when cultured on stroma with various cytokines. In conclusion, the human Lin− UCB SP contains both CD34+ multipotential stem cells and a novel CD7+CD34−Lin− lymphoid progenitor. This observation adds to the growing body of evidence that CD34− progenitors exist in humans.

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