An efficient and rapid method for gene cloning from eukaryotic genomic DNA using overlap-PCR: With an example of cattle Ghrelin gene

2010 ◽  
Vol 391 (3) ◽  
pp. 1490-1493 ◽  
Author(s):  
Ai-ling Zhang ◽  
Li Zhang ◽  
Liang-zhi Zhang ◽  
Hong Chen ◽  
Xian-yong Lan ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Rapid Method ◽  
Genomic Dna ◽  
Ghrelin Gene ◽  
Overlap Pcr

A Rapid Method for the Isolation of Genomic DNA From Aspergillus Fumigatus

1995 ◽  
Vol 25 (4) ◽  
pp. 171-181 ◽  
Author(s):  
Nivedita Bir ◽  
A. Paliwal ◽  
K. Muralidhar ◽  
Prasad Reddy ◽  
P. Usha Sarma
Keyword(s):  
Aspergillus Fumigatus ◽  
Rapid Method ◽  
Genomic Dna

scholarly journals A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue

BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 240-244
Author(s):  
Shan Wei ◽  
Brynn Levy ◽  
Nataly Hoffman ◽  
Claudia Cujar ◽  
Reunet Rodney-Sandy ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rapid Method ◽  
Human Tissue ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Clinical Applications ◽  
Mammalian Tissue ◽  
Fume Hood ◽  
Molecular Assays ◽  
Tissue Specimens

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.

The mouse Lect2 gene: cloning of cDNA and genomic DNA, structural characterization and chromosomal localization

Gene ◽  
1998 ◽  
Vol 216 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Satoshi Yamagoe ◽  
Toshio Watanabe ◽  
Satoshi Mizuno ◽  
Kazuo Suzuki
Keyword(s):  
Gene Cloning ◽  
Structural Characterization ◽  
Genomic Dna ◽  
Chromosomal Localization

scholarly journals A rapid method for isolation of genomic DNA from food-borne fungal pathogens

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
S. Umesha ◽  
H. M. Manukumar ◽  
Sri Raghava
Keyword(s):  
Rapid Method ◽  
Genomic Dna ◽  
Fungal Pathogens ◽  
Food Borne

scholarly journals A simple and rapid method for the preparation of gram-negative bacterial genomic DNA

1993 ◽  
Vol 21 (9) ◽  
pp. 2260-2260 ◽  
Author(s):  
Wen-ping Chen ◽  
Tsong-teh Kuo
Keyword(s):  
Rapid Method ◽  
Genomic Dna ◽  
Gram Negative
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