scholarly journals A rapid method for isolation of genomic DNA from food-borne fungal pathogens

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
S. Umesha ◽  
H. M. Manukumar ◽  
Sri Raghava
Keyword(s):  
Rapid Method ◽  
Genomic Dna ◽  
Fungal Pathogens ◽  
Food Borne

A Rapid Method for the Isolation of Genomic DNA From Aspergillus Fumigatus

1995 ◽  
Vol 25 (4) ◽  
pp. 171-181 ◽  
Author(s):  
Nivedita Bir ◽  
A. Paliwal ◽  
K. Muralidhar ◽  
Prasad Reddy ◽  
P. Usha Sarma
Keyword(s):  
Aspergillus Fumigatus ◽  
Rapid Method ◽  
Genomic Dna

scholarly journals A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue

BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 240-244
Author(s):  
Shan Wei ◽  
Brynn Levy ◽  
Nataly Hoffman ◽  
Claudia Cujar ◽  
Reunet Rodney-Sandy ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Rapid Method ◽  
Human Tissue ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Clinical Applications ◽  
Mammalian Tissue ◽  
Fume Hood ◽  
Molecular Assays ◽  
Tissue Specimens

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.

Development of a novel approach for the production of dried genomic DNA for use as standards for qualitative PCR testing of food-borne pathogens

2004 ◽  
Vol 9 (11-12) ◽  
pp. 695-699 ◽  
Author(s):  
Stefanie Trapmann ◽  
Paolo Catalani ◽  
Jeffrey Hoorfar ◽  
Jozsef Prokisch ◽  
Pierre van Iwaarden ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Food Borne Pathogens ◽  
Novel Approach ◽  
Food Borne ◽  
Qualitative Pcr

scholarly journals Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Salmonella spp. in Terms of Sensitivity and Applicability

2020 ◽  
pp. 1-5
Author(s):  
Yanhong Liu ◽  
Deguo Wang ◽  
Meng Zhang ◽  
Yanhong Liu ◽  
Yongzhen Wang
Keyword(s):  
Genomic Dna ◽  
Pathogenic Bacteria ◽  
Detection Limits ◽  
Isothermal Amplification ◽  
Detection Methods ◽  
23S Rrna ◽  
Lamp Method ◽  
Salmonella Spp ◽  
Loop Mediated Isothermal Amplification ◽  
Food Borne

Salmonella spp. are important food-borne pathogens that can cause diseases in humans. Many detection methods have been established in Salmonella spp. using loop-mediated isothermal amplification (LAMP) or reverse transcription loop-mediated isothermal amplification (RT-LAMP). The detection limits of these assays varied from 1 CFU/reaction to 104 CFU/reaction, from 100 fg genomic DNA/reaction to 10 pg genomic DNA/reaction, or from 2.0×101 CFU/mL to 107 CFU/mL for food samples. In this study, LAMP assays were developed using genomic DNA for the detection of Salmonella spp. Two sets of LAMP primers were designed using the invA gene and the 16S-23S rRNA intergenic spacer region (ITS) of S. enterica as the target sequences for two LAMP assays. The detection limits of the two methods were respectively 20 pg S. enterica DNA/reaction and 10 pg S. enterica DNA/reaction at the optimized temperature, and the LAMP methods were of high repeatability and specificity for S. enterica detection. This study provides a baseline for the application of LAMP for the detection of food-borne pathogenic bacteria.

scholarly journals A simple and rapid method for the preparation of gram-negative bacterial genomic DNA

1993 ◽  
Vol 21 (9) ◽  
pp. 2260-2260 ◽  
Author(s):  
Wen-ping Chen ◽  
Tsong-teh Kuo
Keyword(s):  
Rapid Method ◽  
Genomic Dna ◽  
Gram Negative

Monochrome LightCycler PCR assay for detection and quantification of five common species of Candida and Aspergillus

2005 ◽  
Vol 54 (3) ◽  
pp. 243-248 ◽  
Author(s):  
Rong Bu ◽  
Rajeev K Sathiapalan ◽  
Muna M Ibrahim ◽  
Ibrahim Al-Mohsen ◽  
Edna Almodavar ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Fungal Pathogens ◽  
Low Cost ◽  
False Negative ◽  
Common Species ◽  
Fungal Species ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Negative Results ◽  
Identification And Quantification

Invasive fungal pathogens, especially in immunocompromised hosts, can result in life-threatening infections. Current laboratory/radiological methods for fungal identification are time-consuming and lack sensitivity and specificity. A monochrome, multiplex, real-time PCR assay for the identification and quantification of Candida albicans, Candida krusei, Candida tropicalis, Aspergillus flavus and Aspergillus fumigatus is described here. Detection of each of these fungi was specific and demonstrated 100 % concordance with biochemical/culture identification in all 60 isolates tested. Samples from 16 febrile neutropenic patients with haematological malignancies were also analysed and the utility of the assay in clinical samples was reconfirmed without false-negative results. The sensitivity of this assay was 0.1 pg fungal genomic DNA, corresponding to three cells, for C. albicans, C. krusei, C. tropicalis and A. flavus, and 0.01 pg fungal genomic DNA, i.e. less than one cell, for A. fumigatus. The analysis allows a low-cost, simple, rapid and sensitive alternative for clinical identification and quantification of these five common fungal species.

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