Involvement of Ly6C, 4-1BB, and KLRG1 in the activation of lamina propria lymphocytes in the small intestine of sanroque mice

During early neonatal life, important changes occur in the gut. The intestine is challenged by both milk and a microbial flora. Later on, at weaning, the diet of mice changes from milk to pelleted food leading to changes in microbial contents. This period seems essential for a complete development of the mucosal immune system. We investigated the development of both intraepithelial (IEL) and lamina propria lymphocytes (LPL), from day 5, and every 5 days, up to day 30 after birth. IEL and LPL were isolated from the small intestine and the phenotype was assessed by FACS analyses, using antibodies for detection of T-cell markers CD3, TCRαβ, TCRγδ, CD4, CD8α, CD8β, CD5, CD18, CD54, and CD49d. Our data show a clear increase in the number of LPL just before weaning, while the number of IEL increased after day 15. A more mature pattern of membrane antigen expression of both IEL and LPL was observed at weaning. The adhesion molecules CD18, CD54, and CD49d, essential for cellular communication of lymphocytes, showed an expression peak at weaning. In conclusion, the mouse mucosal immune system develops during the first 3 weeks of neonatal life leading to the formation of a more mature immune system at weaning.

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The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140464 ◽

1995 ◽

Vol 53 ◽

pp. 818-819

Author(s):

D.S. Friend ◽

N. Ghildyal ◽

M.F. Gurish ◽

K.F. Austen ◽

R.L. Stevens

Keyword(s):

Small Intestine ◽

Mast Cells ◽

Epithelial Cells ◽

Lamina Propria ◽

Trichinella Spiralis ◽

Intestinal Epithelial ◽

Carboxypeptidase A ◽

C3h Mice ◽

Granule Morphology ◽

Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

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Faculty Opinions recommendation of Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1092481.546926 ◽

2007 ◽

Author(s):

Arthur Hurwitz

Keyword(s):

Dendritic Cells ◽

Small Intestine ◽

Retinoic Acid ◽

Lamina Propria ◽

De Novo

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Mucosal immunosuppression by induction of apoptosis in lamina propria lymphocytes

Gastroenterology ◽

10.1016/0016-5085(95)27861-3 ◽

1995 ◽

Vol 108 (4) ◽

pp. A889

Keyword(s):

Lamina Propria ◽

Induction Of Apoptosis ◽

Lamina Propria Lymphocytes

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Pathology of Neonatal Calf Diarrhea Induced by a Reo-Like Virus

Veterinary Pathology ◽

10.1177/0300985871008005-00612 ◽

1971 ◽

Vol 8 (5-6) ◽

pp. 490-505 ◽

Cited By ~ 83

Author(s):

C. A. Mebus ◽

E. L. Stair ◽

N. R. Underdahl ◽

M. J. Twiehaus

Keyword(s):

Cell Culture ◽

Small Intestine ◽

Epithelial Cells ◽

Lamina Propria ◽

Diarrhea Virus ◽

Neonatal Calf ◽

Calf Diarrhea ◽

Neonatal Calf Diarrhea ◽

Microscopic Findings

Gross, immunofluorescent, and light microscopic findings in seven gnotobiotic calves inoculated orally with a Reo-like neonatal calf diarrhea virus were compared to findings in three control gnotobiotic calves. Neonatal calf diarrhea virus infected primarily the villous epithelium of the small intestine. Calves examined within 1.5 h after onset of diarrhea had tall columnar immunofluorescent villous epithelial cells in the middle and lower small intestine. Calves examined 2–4.5 h after onset of diarrhea had cuboidal to squamous villous epithelial cells and an increase in reticulum-like cells in the villous lamina propria of the middle and lower small intestine. Viral tilers were 106 and 108 in colonic contents from two calves inoculated with cell-culture-adapted virus and necropsied, respectively, 2 and 6 h after onset of diarrhea.

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