Weak-acid transport in the small intestine: Discrimination in the lamina propria

1981 ◽  
Vol 59 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Chung-Yui Tai ◽  
Michael J. Jackson
Keyword(s):  
Small Intestine ◽  
Lamina Propria ◽  
Weak Acid ◽  
Acid Transport

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

1995 ◽  
Vol 53 ◽  
pp. 818-819
Author(s):  
D.S. Friend ◽  
N. Ghildyal ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Small Intestine ◽  
Mast Cells ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Carboxypeptidase A ◽  
C3h Mice ◽  
Granule Morphology ◽  
Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

Pathology of Neonatal Calf Diarrhea Induced by a Reo-Like Virus

1971 ◽  
Vol 8 (5-6) ◽  
pp. 490-505 ◽  
Author(s):  
C. A. Mebus ◽  
E. L. Stair ◽  
N. R. Underdahl ◽  
M. J. Twiehaus
Keyword(s):  
Cell Culture ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Diarrhea Virus ◽  
Neonatal Calf ◽  
Calf Diarrhea ◽  
Neonatal Calf Diarrhea ◽  
Microscopic Findings

Gross, immunofluorescent, and light microscopic findings in seven gnotobiotic calves inoculated orally with a Reo-like neonatal calf diarrhea virus were compared to findings in three control gnotobiotic calves. Neonatal calf diarrhea virus infected primarily the villous epithelium of the small intestine. Calves examined within 1.5 h after onset of diarrhea had tall columnar immunofluorescent villous epithelial cells in the middle and lower small intestine. Calves examined 2–4.5 h after onset of diarrhea had cuboidal to squamous villous epithelial cells and an increase in reticulum-like cells in the villous lamina propria of the middle and lower small intestine. Viral tilers were 106 and 108 in colonic contents from two calves inoculated with cell-culture-adapted virus and necropsied, respectively, 2 and 6 h after onset of diarrhea.

Immune disorders of the gastrointestinal tract

2010 ◽  
pp. 2244-2256
Author(s):  
D.J. Unsworth
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
B Lymphocytes ◽  
Lamina Propria ◽  
Thoracic Duct ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Immunoglobulin A ◽  
Systemic Circulation ◽  
Lymphoid Follicles

The gastrointestinal tract is protected by gut-associated lymphoid tissue that provides an environment where interaction occurs between luminal antigen and specially adapted immune tissue in Peyer’s patches (small intestine only) or lymphoid follicles. T and B lymphocytes primed in the gut migrate into the systemic circulation via the thoracic duct but home preferentially to the lamina propria of the intestine. Plasma cells of the lamina propria secrete immunoglobulin A as a dimer linked by a joining peptide....

Transport of butyric acid in vascularly perfused anuran small intestine: importance of pH and anion transport

1986 ◽  
Vol 250 (4) ◽  
pp. G469-G474
Author(s):  
D. Hollander ◽  
E. M. Gerard ◽  
C. A. Boyd
Keyword(s):  
Small Intestine ◽  
Butyric Acid ◽  
Nonlinear Function ◽  
Anion Transport ◽  
Transport Protein ◽  
Disulfonic Acid ◽  
Flux Analysis ◽  
Acid Transport ◽  
Distal Small Intestine ◽  
Proximal Small Intestine

Butyric acid transport was studied in the isolated, vascularly perfused frog small intestine. At luminal butyric acid concentrations of 5-50 mM, absorption was a nonlinear function of the luminal concentration, whereas the relationship of absorption to concentration remained linear at 0-1,000 microM. The most important factor regulating the rate and direction of butyric acid transport was the pH. We used unidirectional flux analysis to determine net transport across the epithelium while the pH of the luminal or vascular compartments was changed. We found a four- to fivefold decrease in butyric acid transport into the portal circulation as the lumen pH was increased from 6.0 to 8.0. The pH of the vascular perfusate influenced the vascular-to-lumen transport of butyric acid in the same proportions. The second important regulatory factor of butyric acid transport was the 4,4'-diisothiocyananostilbene-2,2'-disulfonic acid (DIDS)-sensitive anion transport protein. DIDS added to the lumen at 10(-6) M decreased butyric acid transport by approximately 40% at pH 7.4. DIDS also inhibited butyric acid transport when added to the vascular perfusate or when transport was measured in a vascular-to-lumen direction. We suggest that, at the relatively low pH of the proximal small intestine, butyric acid becomes protonated and lipophilic and is mainly transported directly through the cell membrane. At the more alkaline pH of the distal small intestine butyric acid is in the ionized form and transport by the DIDS-sensitive anion transport protein may predominate.

scholarly journals Subepithelial fibroblast cell lines from different levels of gut axis display regional characteristics

1998 ◽  
Vol 274 (5) ◽  
pp. G945-G954 ◽  
Author(s):  
Michelina Plateroti ◽  
Deborah C. Rubin ◽  
Isabelle Duluc ◽  
Renu Singh ◽  
Charlotte Foltzer-Jourdainne ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Lamina Propria ◽  
Cell Lines ◽  
Transforming Growth Factor ◽  
Mesenchymal Cell ◽  
Proximal Colon ◽  
Proximal Jejunum ◽  
Distal Small Intestine

The intestine is characterized by morphofunctional differences along the proximodistal axis. The aim of this study was to derive mesenchymal cell lines representative of the gut axis. We isolated and cloned rat intestinal subepithelial myofibroblasts raised from 8-day proximal jejunum, distal ileum, and proximal colon lamina propria. Two clonal cell lines from each level of the gut were characterized. They 1) express the specific markers vimentin, smooth muscle α-actin, and smooth muscle myosin heavy chain, revealed by immunofluorescence microscopy and 2) distinctly support endodermal cell growth in a coculture model, depending on their regional origin, and 3) the clones raised from the various proximodistal regions maintain the same pattern of morphogenetic and growth and/or differentiation factor gene expression as in vivo: hepatocyte growth and/or scatter factor and transforming growth factor-β1 mRNAs analyzed by RT-PCR were more abundant, in the colon and ileal clones and mucosal connective tissue, respectively. In addition, epimorphin mRNA studied by Northern blot was also the highest in one ileal clone, in which it was selectively upregulated by all-trans retinoic acid (RA) treatment. Epimorphin expression in isolated 8-day intestinal lamina propria was higher in the distal small intestine and proximal colon than in the proximal small intestine. In conclusion, we isolated and characterized homogeneous cell subtypes that can now be used to approach the molecular regulation of the epithelium-mesenchyme-dependent regional specificity along the gut.

Effect of insulin on sugar and amino acid transport cross isolated small intestine

1969 ◽  
Vol 217 (1) ◽  
pp. 53-57 ◽  
Author(s):  
D Fromm ◽  
M Field ◽  
W Silen
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Acid Transport

A student laboratory exercise illustrating active transport across the small intestine

1965 ◽  
Vol 20 (5) ◽  
pp. 1102-1105 ◽  
Author(s):  
T. Hastings Wilson ◽  
Angela DeCarlo
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Intrinsic Factor ◽  
Intestinal Transport ◽  
Student Interest ◽  
Acid Transport ◽  
Single Experiment ◽  
Amino Acid Transport System ◽  
Student Laboratory

A 7-year experience with student laboratory exercises dealing with intestinal transport in vitro is described. A single experiment which has evolved from trial and error in the student laboratory is given in detail. Everted sacs of small intestine from the golden hamster are incubated in flasks containing bicarbonate-saline with 10 mm glucose and 2 mm d- or l-tyrosine. Glucose and l-tyrosine are transported across the intestinal wall against concentration gradients; d-tyrosine is not, illustrating the stereospecificity of the amino acid transport system. A second experiment which illustrates the stimulation of vitamin B12 absorption by gastric intrinsic factor is briefly recorded. The student interest aroused and the high degree of satisfactory results make such experiments useful additions to the repertoire of student laboratory exercises in physiology. intestinal transport; amino acid transport; B12 transport Submitted on November 12, 1964

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