Administration of plasmacytoid dendritic cell-stimulative lactic acid bacteria enhances antigen-specific immune responses

Abstract Type 1 interferon–producing cells (IPCs), also known as plasmacytoid dendritic cell (DC) precursors, represent the key effectors in antiviral innate immunity and triggers for adaptive immune responses. IPCs play important roles in the pathogenesis of systemic lupus erythematosus (SLE) and in modulating immune responses after hematopoietic stem cell transplantation. Understanding IPC development from hematopoietic progenitor cells (HPCs) may provide critical information in controlling viral infection, autoimmune SLE, and graft-versus-host disease. FLT3-ligand (FLT3-L) represents a key IPC differentiation factor from HPCs. Although hematopoietic cytokines such as interleukin-3 (IL-3), IL-7, stem cell factor (SCF), macrophage–colony-stimulating factor (M-CSF), and granulocyte M-CSF (GM-CSF) promote the expansion of CD34+ HPCs in FLT3-L culture, they strongly inhibit HPC differentiation into IPCs. Here we show that thrombopoietin (TPO) cooperates with FLT3-L, inducing CD34+ HPCs to undergo a 400-fold expansion in cell numbers and to generate more than 6 × 106 IPCs per 106 CD34+ HPCs within 30 days in culture. IPCs derived from HPCs in FLT3-L/TPO cultures display blood IPC phenotype and have the capacity to produce large amounts of interferon-α (IFN-α) and to differentiate into mature DCs. This culture system, combined with the use of adult peripheral blood CD34+ HPCs purified from G-CSF–mobilized donors, permits the generation of more than 109 IPCs from a single blood donor.

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Avian reovirus sigma C enhances the mucosal and systemic immune responses elicited by antigen-conjugated lactic acid bacteria

Vaccine ◽

10.1016/j.vaccine.2012.04.043 ◽

2012 ◽

Vol 30 (33) ◽

pp. 5019-5029 ◽

Cited By ~ 11

Author(s):

Kuan-Hsun Lin ◽

Ai-Ping Hsu ◽

Jui-Hung Shien ◽

Tien-Jye Chang ◽

Jiunn-Wang Liao ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Immune Responses ◽

Avian Reovirus

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Effect of IFN-A on immune responses and plasmacytoid dendritic cell functions of patients with hepatitis B

Biomedical Research ◽

10.4066/biomedicalresearch.29-17-3861 ◽

2018 ◽

Vol 29 (6) ◽

Author(s):

Yue Chen ◽

Jiaen Yang ◽

Jingmo Tang ◽

Qianguo Mao ◽

Qizhong Zheng ◽

...

Keyword(s):

Dendritic Cell ◽

Hepatitis B ◽

Immune Responses ◽

Plasmacytoid Dendritic Cell ◽

Cell Functions

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Differential effects of lactobacilli on activation and maturation of mouse dendritic cells

Beneficial Microbes ◽

10.3920/bm2013.0066 ◽

2014 ◽

Vol 5 (3) ◽

pp. 323-334 ◽

Cited By ~ 10

Author(s):

I. Elawadli ◽

J.T. Brisbin ◽

B.A. Mallard ◽

M.W. Griffiths ◽

M. Corredig ◽

...

Keyword(s):

Dendritic Cells ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Immune Responses ◽

Inflammatory Cytokine ◽

Cytokine Production ◽

Lactobacillus Helveticus ◽

Inflammatory Cytokine Production

Lactic acid bacteria (LAB) are of interest because of their potential to modulate immune responses. The effects of LAB range from regulation to stimulation of the immune system. A series of studies were performed in vitro to study the effects of six lactic acid bacteria (LAB), Lactobacillus helveticus LH-2, Lactobacillus acidophilus La-5, La-115, La-116 and La-14, and Lactobacillus salivarius, on maturation and activation of mouse dendritic cells. Production of tumour necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 by dendritic cells (DCs) was determined after treating cells with live LAB. The expression of DC maturation markers, CD80 and CD40, was also measured using flow cytometry after stimulation with LAB. In addition, the expression of Toll-like receptors (TLRs) 2, 4 and 9 by DCs stimulated with LAB was measured. Our results revealed that LAB act differentially on pro-inflammatory and anti-inflammatory cytokine production and induction of co-stimulatory molecules by DCs. Specifically, L. salivarius was found to be the most effective LAB to induce pro-inflammatory cytokine production and expression of co-stimulatory molecules. Moreover, La-14, La-116 and La-5 induced moderate maturation and activation of DCs. On the other hand, LH-2 and La-115 were the least effective lactobacilli to induce DC responses. The present study also revealed that L. salivarius was able to induce the expression of TLR2, 4 and 9 by DCs. In conclusion, various strains and species of LAB can differentially regulate DC activation and maturation, providing further evidence that these bacteria may have the ability to influence and steer immune responses in vivo.

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