Crystal structure of the Ube2K/E2-25K and K48-linked di-ubiquitin complex provides structural insight into the mechanism of K48-specific ubiquitin chain synthesis

2018 ◽  
Vol 506 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Jung-Gyu Lee ◽  
Hyung-Seop Youn ◽  
Jung Youn Kang ◽  
Sam-Yong Park ◽  
Akinori Kidera ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Ubiquitin Chain ◽  
Structural Insight ◽  
Chain Synthesis ◽  
Insight Into

Structural Insight Into Protein Binding of Boron Tracedrug UTX-97 Revealed by the Co-Crystal Structure With Lysozyme at 1.26 Å Resolution

2016 ◽  
Vol 105 (8) ◽  
pp. 2298-2301
Author(s):  
Yukio Morimoto ◽  
Hideko Nagasawa ◽  
Yoshihiro Uto ◽  
Toshiyuki Chatake ◽  
Hitoshi Hori
Keyword(s):  
Crystal Structure ◽  
Protein Binding ◽  
Structural Insight ◽  
Insight Into

Structural Insight into the Dioxygenation of Nitroarene Compounds: the Crystal Structure of Nitrobenzene Dioxygenase

2005 ◽  
Vol 348 (5) ◽  
pp. 1139-1151 ◽  
Author(s):  
Rosmarie Friemann ◽  
Maja M. Ivkovic-Jensen ◽  
Daniel J. Lessner ◽  
Chi-Li Yu ◽  
David T. Gibson ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Structural Insight ◽  
Insight Into

scholarly journals Bag6 complex contains a minimal tail-anchor–targeting module and a mock BAG domain

2014 ◽  
Vol 112 (1) ◽  
pp. 106-111 ◽  
Author(s):  
Jee-Young Mock ◽  
Justin William Chartron ◽  
Ma’ayan Zaslaver ◽  
Yue Xu ◽  
Yihong Ye ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Endoplasmic Reticulum ◽  
Complex Network ◽  
Binding Sites ◽  
Transmembrane Domain ◽  
Degradation Pathway ◽  
Tetratricopeptide Repeat ◽  
Endoplasmic Reticulum Associated Degradation ◽  
Structural Insight ◽  
Insight Into

BCL2-associated athanogene cochaperone 6 (Bag6) plays a central role in cellular homeostasis in a diverse array of processes and is part of the heterotrimeric Bag6 complex, which also includes ubiquitin-like 4A (Ubl4A) and transmembrane domain recognition complex 35 (TRC35). This complex recently has been shown to be important in the TRC pathway, the mislocalized protein degradation pathway, and the endoplasmic reticulum-associated degradation pathway. Here we define the architecture of the Bag6 complex, demonstrating that both TRC35 and Ubl4A have distinct C-terminal binding sites on Bag6 defining a minimal Bag6 complex. A crystal structure of the Bag6–Ubl4A dimer demonstrates that Bag6–BAG is not a canonical BAG domain, and this finding is substantiated biochemically. Remarkably, the minimal Bag6 complex defined here facilitates tail-anchored substrate transfer from small glutamine-rich tetratricopeptide repeat-containing protein α to TRC40. These findings provide structural insight into the complex network of proteins coordinated by Bag6.

Crystal structure of Gig2 protein from Candida albicans provides a structural insight into DUF1479 family oxygenases

2020 ◽  
Vol 150 ◽  
pp. 1272-1280
Author(s):  
Priya Rani ◽  
Gunjan Gautam ◽  
Tamanna Anwar ◽  
Samudrala Gourinath ◽  
Asis Datta
Keyword(s):  
Crystal Structure ◽  
Candida Albicans ◽  
Structural Insight ◽  
Insight Into

scholarly journals Structural Insight into the Antibiotic Action of Telithromycin against Resistant Mutants

2003 ◽  
Vol 185 (14) ◽  
pp. 4276-4279 ◽  
Author(s):  
Rita Berisio ◽  
Joerg Harms ◽  
Frank Schluenzen ◽  
Raz Zarivach ◽  
Harly A. S. Hansen ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Deinococcus Radiodurans ◽  
Ribosomal Subunit ◽  
Large Ribosomal Subunit ◽  
Resistant Strains ◽  
Structural Insight ◽  
Resistant Mutants ◽  
Exit Tunnel ◽  
Structural Insights ◽  
Insight Into

ABSTRACT The crystal structure of the ketolide telithromycin bound to the Deinococcus radiodurans large ribosomal subunit shows that telithromycin blocks the ribosomal exit tunnel and interacts with domains II and V of the 23S RNA. Comparisons to other clinically relevant macrolides provided structural insights into its enhanced activity against macrolide-resistant strains.

Crystal Structure of the Catalytic Domain of Human MAP Kinase Phosphatase 5: Structural Insight into Constitutively Active Phosphatase

2006 ◽  
Vol 360 (5) ◽  
pp. 946-955 ◽  
Author(s):  
Dae Gwin Jeong ◽  
Tae-Sung Yoon ◽  
Jae Hoon Kim ◽  
Mi Young Shim ◽  
Suk-Kyeong Jung ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Map Kinase ◽  
Catalytic Domain ◽  
Map Kinase Phosphatase ◽  
Structural Insight ◽  
Insight Into ◽  
Constitutively Active

scholarly journals Crystal Structure of the Epo1-Bem3 Complex for Bud Growth

2021 ◽  
Vol 22 (8) ◽  
pp. 3812
Author(s):  
Jin Wang ◽  
Lei Li ◽  
Zhenhua Ming ◽  
Lijie Wu ◽  
Liming Yan
Keyword(s):  
Crystal Structure ◽  
Endoplasmic Reticulum ◽  
Membrane Protein ◽  
Protein Complex ◽  
Molecular Architecture ◽  
Hydrogen Deuterium ◽  
Bud Growth ◽  
Structural Insight ◽  
Guanosine Triphosphatase ◽  
Insight Into

Tubules of the endoplasmic reticulum (ER) spread into the buds of yeast by an actin-based mechanism and, upon entry, become attached to the polarisome, a proteinaceous micro-compartment below the tip of the bud. The minimal tether between polarisome and cortical ER is formed by a protein complex consisting of Epo1, a member of the polarisome, Scs2, a membrane protein of the ER and Cdc42 guanosine triphosphatase-activating protein Bem3. Here, we report the crystal structure of a complex between Epo1 and Bem3. In addition, we characterize through the hydrogen/deuterium (H/D) exchange assay the interface between Scs2 and Epo1. Our findings provide a first structural insight into the molecular architecture of the link between cortical ER and the polarisome.

scholarly journals Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme

2014 ◽  
Vol 42 (6) ◽  
pp. 4113-4122 ◽  
Author(s):  
Dmitrij Golovenko ◽  
Elena Manakova ◽  
Linas Zakrys ◽  
Mindaugas Zaremba ◽  
Giedrius Sasnauskas ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Restriction Enzyme ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Terminal Fragment ◽  
Structural Insight ◽  
Insight Into
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