scholarly journals Stimulation of insulin secretion by acetylenic fatty acids in insulinoma MIN6 cells through FFAR1

2020 ◽  
Vol 522 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Katsutoshi Nishino ◽  
Haruka Uesugi ◽  
Akira Hirasawa ◽  
Anna Ohtera ◽  
Yusaku Miyamae ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Min6 Cells ◽  
Acetylenic Fatty Acids ◽  
Stimulation Of

scholarly journals Palmitic acid acutely inhibits acetylcholine- but not GLP-1-stimulated insulin secretion in mouse pancreatic islets

2010 ◽  
Vol 299 (3) ◽  
pp. E475-E485 ◽  
Author(s):  
Nicolai M. Doliba ◽  
Wei Qin ◽  
Sergei A. Vinogradov ◽  
David F. Wilson ◽  
Franz M. Matschinsky
Keyword(s):  
Fatty Acids ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Insulin Release ◽  
Cell Function ◽  
Hormone Secretion ◽  
Albumin Concentration ◽  
Dependent Manner ◽  
Fixed Concentration ◽  
Stimulation Of

Fatty acids, acetylcholine, and GLP-1 enhance insulin secretion in a glucose-dependent manner. However, the interplay between glucose, fatty acids, and the neuroendocrine regulators of insulin secretion is not well understood. Therefore, we studied the acute effects of PA (alone or in combination with glucose, acetylcholine, or GLP-1) on isolated cultured mouse islets. Two different sets of experiments were designed. In one, a fixed concentration of 0.5 mM of PA bound to 0.15 mM BSA was used; in the other, a PA ramp from 0 to 0.5 mM was applied at a fixed albumin concentration of 0.15 mM so that the molar PA/BSA ratio changed within the physiological range. At a fixed concentration of 0.5 mM, PA markedly inhibited acetylcholine-stimulated insulin release, the rise of intracellular Ca2+, and enhancement of cAMP production but did not influence the effects of GLP-1 on these parameters of islet cell function. 2-ADB, an IP3 receptor inhibitor, reduced the effect of acetylcholine on insulin secretion and reversed the effect of PA on acetylcholine-stimulated insulin release. Islet perfusion for 35–40 min with 0.5 mM PA significantly reduced the calcium storage capacity of ER measured by the thapsigargin-induced Ca2+ release. Oxygen consumption due to low but not high glucose was reduced by PA. When a PA ramp from 0 to 0.5 mM was applied in the presence of 8 mM glucose, PA at concentrations as low as 50 μM significantly augmented glucose-stimulated insulin release and markedly reduced acetylcholine's effects on hormone secretion. We thus demonstrate that PA acutely reduces the total oxygen consumption response to glucose, glucose-dependent acetylcholine stimulation of insulin release, Ca2+, and cAMP metabolism, whereas GLP-1's actions on these parameters remain unaffected or potentiated. We speculate that acute emptying of the ER calcium by PA results in decreased glucose stimulation of respiration and acetylcholine potentiation of insulin secretion.

scholarly journals Cyclosporin A stimulation of glucose-induced insulin secretion in MIN6 cells.

Endocrinology ◽  
1996 ◽  
Vol 137 (12) ◽  
pp. 5255-5263 ◽  
Author(s):  
K Ebihara ◽  
K Fukunaga ◽  
K Matsumoto ◽  
M Shichiri ◽  
E Miyamoto
Keyword(s):  
Insulin Secretion ◽  
Cyclosporin A ◽  
Min6 Cells ◽  
Stimulation Of

scholarly journals Stimulation of Insulin Secretion by Long-Chain Free Fatty Acids. A DIRECT PANCREATIC EFFECT

1973 ◽  
Vol 52 (8) ◽  
pp. 1979-1984 ◽  
Author(s):  
Stephen R. Crespin ◽  
William B. Greenough ◽  
Daniel Steinberg
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Free Fatty Acids ◽  
Long Chain ◽  
Stimulation Of

On the role of glucose-dependent insulintropic polypeptide in postprandial metabolism in humans

2010 ◽  
Vol 298 (3) ◽  
pp. E614-E621 ◽  
Author(s):  
Meena Asmar ◽  
Winnie Tangaa ◽  
Sten Madsbad ◽  
Kristine Hare ◽  
Arne Astrup ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Energy Intake ◽  
Subjective Feeling ◽  
Postprandial Metabolism ◽  
Visual Analog Scales ◽  
Ad Libitum ◽  
Healthy Males ◽  
Stimulation Of

We investigated the role of glucose-dependent insulintropic polypeptide (GIP) in the regulation of gastric emptying (GE), appetite, energy intake (EI), energy expenditure (EE), plasma levels of triglycerides (TAG), and free fatty acids (FFA) in humans. First, 20 healthy males received intravenous infusion of GIP (0.8 pmol·kg−1·min−1) or saline for 300 min during and after a fixed meal ( protocol 1). GE was measured using paracetamol, appetite sensations using visual analog scales, EE using indirect calorimetry, and EI during a subsequent ad libitum meal (at 300 min). Next, 10 healthy males received intravenous infusions of Intralipid, glucose, or Intralipid plus glucose, with and without GIP (1.5 pmol·kg−1·min−1) for 300 min ( protocol 2). In protocol 1, GIP did not have any effect on GE, EI, EE, removal of TAG, or FFA and did not influence the subjective feeling of hunger, satiety, fullness or prospective food consumption compared with saline. In protocol 2, no difference was seen in the plasma TAG on Intralipid + GIP/saline and Intralipid + glucose + GIP/saline days. FFA concentrations were lower on Intralipid + glucose + GIP/saline days ( P < 0.05) compared with Intralipid + GIP/saline days and on Intralipid + GIP day ( P < 0.004) compared with Intralipid + saline day. Insulin increased on all GIP days compared with saline days ( P < 0.05). In conclusion, while confirming its insulinotropic effects, these data suggest that GIP does not affect GE, appetite, energy intake, EE, or the clearance rate of the applied TAG formulation in humans. However, both insulin and GIP lower post-Intralipid FFA concentration, GIP probably via stimulation of insulin secretion, increasing FFA reesterification.

scholarly journals Stimulation of insulin secretion by infusion of free fatty acids

1969 ◽  
Vol 48 (10) ◽  
pp. 1934-1943 ◽  
Author(s):  
Stephen R. Crespin ◽  
William B. Greenough ◽  
Daniel Steinberg
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Free Fatty Acids ◽  
Stimulation Of

Stimulation of residual insulin secretion by glibenclamide in insulin dependent diabetics

1980 ◽  
Vol 95 (3) ◽  
pp. 372-375 ◽  
Author(s):  
B. J. Burke ◽  
R. J. Sherriff
Keyword(s):  
Insulin Secretion ◽  
Insulin Therapy ◽  
Metabolic Control ◽  
C Peptide ◽  
Beneficial Effects ◽  
Insulin Dependent ◽  
Serum And Urine ◽  
Stimulation Of

Abstract. Residual insulin secretion, reflected by the presence of C-peptide in serum and urine, has been demonstrated in 5 of 10 insulin-requiring diabetics of less than 10 years' duration tested. The C-peptide response, in the C-peptide secretors, showed a significant increase in both serum and urine after 4 weeks' treatment with 15 mg glibenclamide daily in addition to their usual insulin regime although no beneficial effects in metabolic control were detected. It is suggested that glibenclamide might be a useful adjunct to insulin therapy in insulinrequiring diabetics who still secrete C-peptide.

Stimulation of insulin secretion from isolated rat islets by SaRI 59-801. Relation to cAMP concentration and Ca2+ uptake

Diabetes ◽  
1985 ◽  
Vol 34 (7) ◽  
pp. 691-695 ◽  
Author(s):  
R. L. Hanson ◽  
C. M. Isaacson
Keyword(s):  
Insulin Secretion ◽  
Camp Concentration ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Isolated Rat ◽  
Stimulation Of

Stimulation of insulin secretion from isolated rat islets by SaRI 59-801

Diabetes ◽  
1985 ◽  
Vol 34 (6) ◽  
pp. 548-552 ◽  
Author(s):  
R. L. Hanson ◽  
C. M. Isaacson ◽  
L. D. Boyajy
Keyword(s):  
Insulin Secretion ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Isolated Rat ◽  
Stimulation Of

scholarly journals GPR40 Is Necessary but Not Sufficient for Fatty Acid Stimulation of Insulin Secretion In Vivo

Diabetes ◽  
2007 ◽  
Vol 56 (4) ◽  
pp. 1087-1094 ◽  
Author(s):  
M. G. Latour ◽  
T. Alquier ◽  
E. Oseid ◽  
C. Tremblay ◽  
T. L. Jetton ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Fatty Acid ◽  
Insulin Secretion In Vivo ◽  
Stimulation Of
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