The impact of intracellular aminopeptidase on C2C12 myoblast proliferation and differentiation

Abstract BackgroundAs a well-known cancer-related miRNA, miR-193b-3p is enriched in skeletal muscle but dysregulated in muscle disease. However, mechanism underpinning has not been addressed so far. MethodsHere, we probed the impact of miR-193b-3p on myogenesis by mainly using goat tissues and skeletal muscle satellite cells (MuSCs), with combined methods including RNA-seq to profile the transcriptome affected by miR-193b-3p, cell-counting kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) for cell proliferation assay, and RNA-RNA dual-labeled fluorescence in situ hybridization (FISH) for RNA colocalization. ResultsmiR-193b-3p is highly enriched in goat skeletal muscles, and ectopic miR-193b-3p promotes MuSCs proliferation and differentiation. Moreover, insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) is the most activated insulin signaling genes when overexpression miR-193b-3p and the miRNA recognition element (MRE) within IGF1BP1 3ʹ untranslated region (UTR) is indispensable for its activation caused by miR-193b-3p. Consistently, expression patterns and function of IGF2BP1 were similar to those of miR-193b-3p in tissues and MuSCs. While the overexpression of miR-193b-3p failed to induce pax7 expression and myoblast proliferation when IGF2BP1 knockdown. Furthermore, miR-193b-3p destabilized IGF2BP1 mRNA but unexpectedly promoted levels of IGF2BP1 heteronuclear RNA (hnRNA) dramatically. Moreover, miR-193b-3p could enhance fly luciferase activity when inserted upstream of its promoter, and induce neighboring genes of itself. However, miR-193b-3p inversely regulated IGF2BP1 and myoblast proliferation in mouse C2C12 myoblast. These data unveil that goat miR-193b-3p promotes myoblast proliferation via activating IGF2BP1 by binding on its 3ʹ UTR.ConclusionsOur novel findings highlight the positive regulation between miRNA and its target genes in muscle development, which further extends the repertoire of miRNA functions.

Download Full-text

Lactoferrin promotes murine C2C12 myoblast proliferation and differentiation and myotube hypertrophy

Molecular Medicine Reports ◽

10.3892/mmr.2018.8603 ◽

2018 ◽

Author(s):

Tomoya Kitakaze ◽

Meiku Oshimo ◽

Yasuyuki Kobayashi ◽

Mizuyuki Ryu ◽

Yasushi Suzuki ◽

...

Keyword(s):

C2c12 Myoblast ◽

Proliferation And Differentiation ◽

Myoblast Proliferation ◽

Myotube Hypertrophy

Download Full-text

The Effects of Marine Algal Polyphenols, Phlorotannins, on Skeletal Muscle Growth in C2C12 Muscle Cells via Smad and IGF-1 Signaling Pathways

Marine Drugs ◽

10.3390/md19050266 ◽

2021 ◽

Vol 19 (5) ◽

pp. 266

Author(s):

Seo-Young Kim ◽

Ji-Hyeok Lee ◽

Nalae Kang ◽

Kil-Nam Kim ◽

You-Jin Jeon

Keyword(s):

Skeletal Muscle ◽

Muscle Growth ◽

Negative Regulator ◽

Growth Effect ◽

C2c12 Myoblast ◽

Synthetic Drugs ◽

Proliferation And Differentiation ◽

Myoblast Proliferation ◽

C2c12 Muscle Cells ◽

Marine Algal

Skeletal muscle is an important tissue in energy metabolism and athletic performance. The use of effective synthetic supplements and drugs to promote muscle growth is limited by various side effects. Moreover, their use is prohibited by anti-doping agencies; hence, natural alternatives are needed. Therefore, we evaluated the muscle growth effect of substances that can act like synthetic supplements from edible marine algae. First, we isolated six marine algal polyphenols belonging to the phlorotannin class, namely dieckol (DK), 2,7′′-phloroglucinol-6,6′-bieckol (PHB), phlorofucofuroeckol A (PFFA), 6,6′-bieckol (6,6-BK), pyrogallol-phloroglucinol-6,6′-bieckol (PPB), and phloroglucinol (PG) from an edible brown alga, Ecklonia cava and evaluated their effects on C2C12 myoblasts proliferation and differentiation. Of the six phlorotannin isolates evaluated, DK and PHB induced the highest degree of C2C12 myoblast proliferation. In addition, DK and PHB regulates myogenesis by down-regulating the Smad signaling, a negative regulator, and up-regulating the insulin-like growth factor-1 (IGF-1) signaling, a positive regulator. Interestingly, DK and PHB bind strongly to myostatin, which is an inhibitor of myoblast proliferation, while also binding to IGF-1 receptors. Moreover, they bind to IGF-1 receptor. These results suggest that DK and PHB are potential natural muscle building supplements and could be a safer alternative to synthetic drugs.

Download Full-text

Enhancement of C2C12 myoblast proliferation and differentiation by GASP-2, a myostatin inhibitor

Biochemistry and Biophysics Reports ◽

10.1016/j.bbrep.2016.03.001 ◽

2016 ◽

Vol 6 ◽

pp. 39-46 ◽

Cited By ~ 4

Author(s):

Luce Pèrié ◽

Alexis Parenté ◽

Caroline Brun ◽

Laetitia Magnol ◽

Patrick Pélissier ◽

...

Keyword(s):

C2c12 Myoblast ◽

Proliferation And Differentiation ◽

Myoblast Proliferation

Download Full-text

Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

Journal of Toxicology ◽

10.1155/2020/1807126 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Piyaporn Surinlert ◽

Nitchamon Kongthong ◽

Mariam Watthanard ◽

Thannicha Sae-lao ◽

Piyawat Sookbangnop ◽

...

Keyword(s):

Muscle Development ◽

Myogenic Differentiation ◽

Styrene Oxide ◽

C2c12 Myoblast ◽

Nuclear Condensation ◽

Cell Cycles ◽

Cycle Arrest ◽

Proliferation And Differentiation ◽

Myoblast Proliferation ◽

Gene Expressing

Contaminations of chemicals in foods and drinks are raising public concerns. Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems. The present study was designed to investigate the effects of styrene monomer (STR) and its metabolite styrene oxide (STO) on C2C12 myoblast proliferation and differentiation. Based on an MTT assay, both STR and STO showed no cytotoxic effect at 10–100 μM. However, at 50–100 μM STO, but not STR, significantly inhibited cell proliferation. The STO-treated cells were accumulated in S-phase of cell cycles as revealed by flow cytometry. The antioxidant enzyme (catalase and superoxide dismutase) activities and the gene expressing these enzymes of the arrested cells were decreased and ultimately led to nuclear condensation and expression of apoptotic markers such as cleaved caspase-3 and-9, but not cleaved caspase-8. In addition, STO significantly suppressed myogenic differentiation by decreasing both the number and size of differentiated myotubes. Biochemical analysis showed attenuations of total protein synthesis and myosin heavy chain (MHC) protein expression. In conclusion, a metabolite of styrene, STO, leached from plastic packaging of foods and beverages suppressed both myoblast proliferation and differentiation, which would affect skeletal muscle development and regeneration.

Download Full-text