Structure and function analysis of a CC-NBS-LRR protein AT1G12290

Background: Heart failure is a growing cause of human morbidity and mortality. Supplementations of free radical scavenging antioxidants have largely failed to protect the myocardium from oxidative stress diseases. While endogenous transcriptional activation of antioxidants appears to be promising, their chronic effects are unknown. Here, we tested a hypothesis that chronic activation of antioxidant system will result in reductive stress (RS) and lead to pathological cardiac hypertrophy. Methods: Novel transgenic (TG) mice expressing constitutively active Nrf2 in the heart (α-MHC-caNrf2-TG) and their littermates were used to study the effects on structure and function of the myocardium. Myocardial glutathione redox state (GSH/GSSG), transcript levels (qPCR), and protein (immunoblotting) for Nrf2-related antioxidants and structure and function analysis (echocardiography - Vevo2100 Imager) in Non-transgenic (NTg), TG-low and TG-high mice (n=6-12/gp.) were performed at 6-8 months of age. Further, changes in cardiomyocytes and rate of survival in TG mice were analyzed. Results: Kaplan-Meier survival plots demonstrated 10 and 40% mortality in TG-low and TG-high, respectively, compared to NTG by 60 weeks of age. The myocardial glutathione and its redox ratio (GSH/GSSG) were significantly increased (p<0.05) in the TG-low and TG-high compared with NTg mice indicates development of RS. A significant increase in Nrf2-ARE (promoter) binding with increased expression of antioxidant genes and proteins (p<0.05) were noted in TG vs. NTg mice. Increased heart-to-body weight and heart weight to tibia length ratios were prominent in TG-high relative to NTg or TG-low mice. Histological analyses (WGA, H&E staining) showed increased cardiomyocyte size, ventricular wall thickening and decreased chamber volume in TG mice. Echocardiography analyses revealed significant hypertrophic cardiomyopathy with abnormally increased ejection fraction (HCM i EF) due to chronic reductive stress. Conclusion: Thus, basal attenuation of the obligatory oxidative signaling with chronic activation of Nrf2-antioxidants could shift the redox equilibrium to “reductive” side and thereby causing pathological cardiac remodeling.

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467 In-silico structure and function analysis of the hepatitis C virus non-structural protein 4B (NS4B)

Journal of Hepatology ◽

10.1016/s0168-8278(05)81879-3 ◽

2005 ◽

Vol 42 ◽

pp. 169

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

In Silico ◽

Function Analysis ◽

Structure And Function ◽

Structural Protein ◽

And Function

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Structure and function analysis of protein–nucleic acid complexes

Russian Chemical Reviews ◽

10.1070/rcr4581 ◽

2016 ◽

Vol 85 (5) ◽

pp. 445-463 ◽

Cited By ~ 2

Author(s):

S A Kuznetsova ◽

T S Oretskaya

Keyword(s):

Nucleic Acid ◽

Function Analysis ◽

Structure And Function ◽

Protein Nucleic Acid ◽

And Function

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Structure and function analysis of the human myeloid cell nuclear differentiation antigen promoter: Evidence for the role of Sp1 and not of c-Myb or PU.1 in myelomonocytic lineage-specific expression

Journal of Cellular Biochemistry ◽

10.1002/(sici)1097-4644(199705)65:2<231::aid-jcb8>3.0.co;2-v ◽

1997 ◽

Vol 65 (2) ◽

pp. 231-244 ◽

Cited By ~ 11

Author(s):

Woei-Yau Kao ◽

Judith A. Briggs ◽

Marsha C. Kinney ◽

Roy A. Jensen ◽

Robert C. Briggs

Keyword(s):

Function Analysis ◽

Myeloid Cell ◽

Structure And Function ◽

Differentiation Antigen ◽

Specific Expression ◽

Nuclear Differentiation ◽

And Function

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Structure-function analysis of Arabidopsis TOPLESS reveals fundamental conservation of repression mechanisms across eukaryotes

10.1101/2020.03.04.976134 ◽

2020 ◽

Author(s):

Alexander R. Leydon ◽

Wei Wang ◽

Hardik P. Gala ◽

Sabrina Gilmour ◽

Samuel Juarez-Solis ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Function Analysis ◽

Structure And Function ◽

Environmental Cues ◽

Auxin Response ◽

Middle Domain ◽

N Terminus ◽

And Function ◽

The Relationship ◽

Repression Domain

SummaryThe plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli is poorly understood. Previously, we had used the N-terminus of Arabidopsis thaliana TPL to enable repression of a synthetic auxin response circuit in Saccharomyces cerevisiae (yeast). Here, we leveraged the yeast system to interrogate the relationship between TPL structure and function, specifically scanning for repression domains. We identified a potent repression domain in Helix 8 located within the CRA domain, which directly interacted with the Mediator middle domain subunits Med21 and Med10. Interactions between TPL and Mediator were required to fully repress transcription in both yeast and plants. In contrast, we found that multimer formation, a conserved feature of many corepressors, had minimal influence on the repression strength of TPL.

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Purana Pura Suniantara Analisis Struktur dan Fungsi

HUMANIS ◽

10.24843/jh.2019.v23.i01.p22 ◽

2019 ◽

pp. 140

Author(s):

Ni Luh Gede Yunita Kazi Wiguna ◽

I Gde Nala Antara ◽

I Ketut Ngurah Sulibra

Keyword(s):

Function Analysis ◽

Structure And Function ◽

Text Structure ◽

Narrative Text ◽

Inductive Method ◽

Moral Value ◽

Language Variety ◽

Faithful Servant ◽

Observation Method ◽

And Function

This research analyzes traditional Balinese literature works in the form of narrative text. The analyzed text is Purana Pura Suniantara Analysis of Structure and Function. It tells the story about Dalem Suniantara Temple during the reign of I Gusti Agung Jambe Tangkeban along with a faithful servant, I Gede Batan Nyuh. This research used structural theory by Teeuw and Nurgiyantoro. Method and technique used is falls into three stages, which are data obtained by using observation method, supported by reading and note taking technique. Data analyzed stage uses qualitative method and analytic descriptive technique, while result provision of data analysis used informal method and supported by deductive as well as inductive method. The result obtained from this research is structure and function of test, including to structure format which are consist of Purana Pura Suniantara teks, as well as its text’s form and language variety. Based on its language variety, Purana Pura Suniantara teks used Kawi – Bali language which is a mixture of Sanskrit, Old Javanese, Middle Javanesse, and Balinese. Narrative text structure consists of plot, background, characterization, theme, and moral value. Further, function analysis consists of historical, social, and religious function.

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