Inhibition of ribosome biogenesis by actinomycin D affects Arabidopsis root development

2021 ◽  
Author(s):  
Yanxue Zhao ◽  
Lei Wang ◽  
Xuwu Sun ◽  
Yiqun Bao ◽  
Hao Liu ◽  
...  
Keyword(s):  
Root Development ◽  
Ribosome Biogenesis ◽  
Actinomycin D ◽  
Arabidopsis Root

SBDS Protein Plays a Role in Ribosome Biogenesis.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 185-185
Author(s):  
Karthik A. Ganapathi ◽  
Karyn M. Austin ◽  
Maggie Malsch ◽  
Akiko Shimamura
Keyword(s):  
Ribosome Biogenesis ◽  
Actinomycin D ◽  
Bone Marrow Failure ◽  
Pancreatic Insufficiency ◽  
Protein Translation ◽  
Exocrine Pancreatic Insufficiency ◽  
Nucleolar Localization ◽  
28S Rrna ◽  
Wild Type ◽  
Shwachman Diamond Syndrome

Abstract Shwachman-Diamond syndrome is an autosomal recessive disorder characterized by exocrine pancreatic insufficiency, bone marrow failure, and leukemia predisposition. The majority of patients with Shwachman-Diamond syndrome harbor mutations in the SBDS gene. SBDS is a novel gene of unknown function and is highly conserved throughout evolution. Studies of the yeast orthologue, YLR022c/SDO1, suggest that SBDS may play a role in ribosome biogenesis. In support of this hypothesis, we have found that the SBDS protein shuttles in and out of the nucleolus. Previously we have shown that SBDS nucleolar localization is regulated in a cell cycle-dependant manner. We now find that SBDS nucleolar localization is also lost following exposure to actinomycin D, suggesting that SBDS nucleolar localization is dependent on active ribosomal RNA (rRNA) transcription. In cell survival assays, SBDS−/− patient-derived cells are sensitive to actinomycin D treatment relative to normal control cells. Introduction of the wild-type SBDS cDNA into SBDS−/− cells corrects their actinomycin D sensitivity, confirming that the observed sensitivity is SBDS-dependent. In contrast, SBDS−/− cells do not exhibit increased sensitivity to cyclohexamide, a protein translation inhibitor. Consistent with this result, SBDS protein co-localizes with ribosomal precursor subunits but not with mature polysomes upon sucrose gradient sedimentation. No differences in polysome profiles are observed between SBDS−/− cells and wild type control cells. Gel filtration studies suggest that SBDS associates into a complex with other proteins. SBDS co-immunoprecipitates with other nucleolar proteins involved in rRNA biogenesis. RNA immunoprecipitation studies reveal that SBDS also associates with the 28S rRNA but not the 18S rRNA. These findings support the hypothesis that SBDS plays a role in ribosome biogenesis

scholarly journals Regulation of Arabidopsis root development by small signaling peptides

2013 ◽  
Vol 4 ◽  
Author(s):  
Christina Delay ◽  
Nijat Imin ◽  
Michael A. Djordjevic
Keyword(s):  
Root Development ◽  
Arabidopsis Root ◽  
Signaling Peptides

scholarly journals The AAA-ATPase MIDASIN 1 Functions in Ribosome Biogenesis and Is Essential for Embryo and Root Development

2019 ◽  
Vol 180 (1) ◽  
pp. 289-304 ◽  
Author(s):  
Peng-Cheng Li ◽  
Ke Li ◽  
Juan Wang ◽  
Chuan-Zhi Zhao ◽  
Shu-Zhen Zhao ◽  
...  
Keyword(s):  
Root Development ◽  
Ribosome Biogenesis ◽  
Aaa Atpase

scholarly journals The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

2013 ◽  
Vol 25 (9) ◽  
pp. 3377-3388 ◽  
Author(s):  
Norma Fàbregas ◽  
Na Li ◽  
Sjef Boeren ◽  
Tara E. Nash ◽  
Michael B. Goshe ◽  
...  
Keyword(s):  
Root Development ◽  
Arabidopsis Root

scholarly journals Roles for Class III HD-Zip and KANADI Genes in Arabidopsis Root Development

2004 ◽  
Vol 135 (4) ◽  
pp. 2261-2270 ◽  
Author(s):  
Nathaniel P. Hawker ◽  
John L. Bowman
Keyword(s):  
Root Development ◽  
Class Iii ◽  
Arabidopsis Root

scholarly journals The volatile cedrene from plant beneficial Trichoderma guizhouense modulates Arabidopsis root development through auxin transport and signaling

2021 ◽  
Author(s):  
Yucong Li ◽  
Jiahui Shao ◽  
Yansong Fu ◽  
Yu Chen ◽  
Hongzhe Wang ◽  
...  
Keyword(s):  
Root Development ◽  
Root Formation ◽  
Auxin Transport ◽  
Plant Root ◽  
Lateral Roots ◽  
Confocal Imaging ◽  
Auxin Receptor ◽  
Arabidopsis Root ◽  
Rhizosphere Microorganisms ◽  
Stimulate Plant Growth

Rhizosphere microorganisms interact with plant roots by producing chemical signals to regulate root development. However, the involved distinct bioactive compounds and the signal transduction pathways are remaining to be identified. Here, we show that sesquiterpenes (SQTs) are the main volatile compounds produced by plant beneficial Trichoderma guizhouense NJAU 4742, inhibition of SQTs synthesis in this strain indicated their involvement in plant-fungus cross-kingdom signaling. SQTs component analysis further identified the cedrene, a high abundant SQT in strain NJAU 4742, could stimulate plant growth and root development. Genetic analysis and auxin transport inhibition showed that auxin receptor TIR1, AFB2, auxin-responsive protein IAA14, and transcription factor ARF7, ARF19 affect the response of lateral roots to cedrene. Moreover, auxin influx carrier AUX1, efflux carrier PIN2 were also indispensable for cedrene-induced lateral root formation. Confocal imaging showed that cedrene affected the expression of pPIN2:PIN2:GFP and pPIN3:PIN3:GFP, which may be related to the effect of cedrene on root morphology. These results suggest that a novel SQT molecule from plant beneficial T. guizhouense can regulate plant root development through auxin transport and signaling.

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